Skip to main content Accessibility help

Spatio-temporal expression analysis of two kinds of chemical communication-related proteins in the worker bee Apis cerana cerana Fabricius (Hymenoptera: Apidae)

  • Li Hong-Liang (a1) (a2), Zhang Ya-Li (a3), Wang Hai-Yan (a2), Gao Qi-Kang (a2) and Cheng Jia-An (a2)...


The spatio-temporal expressed profiles of two kinds of chemical communication-related protein genes, the odorant-binding protein of Ac-ASP2 and chemosensory protein of Ac-ASP3, were identified by real-time polymerase chain reaction (PCR). Results obtained using the 2−ΔΔCt method showed that Ac-ASP2 was a gene coding antenna-specific protein that did not express in larvae and pupae, but had discontinuous high abundance periods at 1, 9, 15, 27 and 30 days. The expressing abundance at such periods was at least ten times higher than that at other periods. From the distribution of Ac-ASP3 mRNA observed in different tissues, the transcript levels seemed to be higher in the wings, abdomen and thorax (of order ~106), and lower in the legs, antennae and head (of order ~105). From highest to lowest, the original copy number was found in the various body parts in the following order: wings, abdomen, thorax, legs, antenna, and head. The results suggest that Ac-ASP3 has an intimate relation with the chemosensory behaviour of wings and abdomen in Apis cerana cerana.


Corresponding author

*Corresponding author. E-mail:


Hide All
Chantawannakul, P, Ward, L, Boonham, N and Brown, M (2006) A scientific note on the detection of honeybee viruses using real-time PCR (TaqMan) in Varroa mites collected from a Thai honeybee (Apis mellifera) apiary. Journal of Invertebrate Pathology 91: 6973.
Chen, SL (2001) Apiculture Science in China. Beijing, China: Agricultural Publishing House of China, pp.123131 (in Chinese).
Forêt, S and Maleszka, R (2006) Function and evolution of a gene family encoding odorant binding-like proteins in a social insect, the honey bee (Apis mellifera). Genome Research 16: 14041413.
Forêt, S, Wanner, KW and Maleszka, R (2007) Chemosensory proteins in the honey bee: Insights from the annotated genome, comparative analyses and expressional profiling. Insect Biochemistry and Molecular Biology 37: 1928.
Kucharski, R and Maleszka, R (2005) Microarray and real-time PCR analyses of gene expression in the honeybee brain following caffeine treatment. Journal of Molecular Neuroscience 27: 269276.
Kukielka, D, Esperon, F, Higes, M and Sanchez-Vizcaino, JM (2008) A sensitive one-step real-time RT-PCR method for detection of deformed wing virus and black queen cell virus in honeybee Apis mellifera. Journal of Virological Methods 147: 275281.
Li, HL, Lou, BG, Cheng, JA and Gao, QK (2007) The chemosensory protein of the Chinese honeybee, Apis cerana cerana: molecular cloning of cDNA, immunocytochemical localization and expression. Chinese Science Bulletin 52: 13551364.
Li, HL, Zhang, YL, Gao, QK, Cheng, JA and Lou, BG (2008) Molecular Identification of cDNA, immunolocalization, and expression of a putative odorant-binding protein from an Asian honeybee, Apis cerana cerana. Journal of Chemical Ecology 34: 15931601.
Livak, KJ and Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402408.
Michelette, ERF and Soares, AEE (1993) Characterization of preimaginal developmental stages in Africanized honey bee workers (Apis mellifera L.). Apidologie 24: 431440.
Pelosi, P (1996) Perireceptor events in olfaction. Journal of Neurobiology 30: 319.
Pelosi, P, Zhou, JJ, Ban, LP and Calvello, M (2006) Soluble proteins in insect chemical communication. Cellular and Molecular Life Sciences 63: 16581676.
Rachinsky, A, Strambi, C, Strambi, A and Hartfelder, K (1990) Caste and metamorphosis: hemolymph titers of juvenile hormone and ecdysteroids in last instar honeybee larvae. General and Comparative Endocrinology 79: 3138.
Rheault, MR, Okech, BA, Keen, SB et al. (2007) Molecular cloning, phylogeny and localization of AgNHA1: the first Na+/H+ antiporter (NHA) from a metazoan, Anopheles gambiae. Journal Experimental Biology 210: 38483861.
Yang, CH (1998) The development and lives of male and worker bees of Apis cerana cerana. Journal of Honey Bee 8: 16 (in Chinese).


Spatio-temporal expression analysis of two kinds of chemical communication-related proteins in the worker bee Apis cerana cerana Fabricius (Hymenoptera: Apidae)

  • Li Hong-Liang (a1) (a2), Zhang Ya-Li (a3), Wang Hai-Yan (a2), Gao Qi-Kang (a2) and Cheng Jia-An (a2)...


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed