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Simultaneous detection of bovine- and sheep-derived materials in feedstuffs by PCR

  • Wen Wei-Gang (a1), Zhao Xiu-Ling (a1), He Shui-Shan (a1), Du Hua-Hong (a1) and Huang Shao-Tang (a1)...


To control the spread of bovine spongiform encephalopathy and scrapie, a method was developed for the simultaneous detection of bovine- and sheep-derived materials in feedstuffs. Chelex-100 was adopted for the fast extraction of animal genomic DNA. A pair of universal primers, designed on the basis of homology in sequences of bovine and sheep mtDNA, was used for PCR. This method is of great value to quarantine departments for its simplicity and rapid detection.


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