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Rescue of H5N1 subtype avian influenza A virus lethal to mice

Published online by Cambridge University Press:  15 June 2007

Pan Wei-Qi ,
Liu Ming ,
Zhang Yun ,
Liu Chun-Guo ,
Yang Tao  and
Li Jing-Peng
Pan Wei-Qi
Affiliation:
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Harbin 150001, China Northeast Agricultural University, Harbin 150030, China
Liu Ming*
Affiliation:
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Harbin 150001, China
Zhang Yun
Affiliation:
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Harbin 150001, China
Liu Chun-Guo
Affiliation:
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Harbin 150001, China
Yang Tao
Affiliation:
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Harbin 150001, China
Li Jing-Peng
Affiliation:
Northeast Agricultural University, Harbin 150030, China
*
*Corresponding author. E-mail: liuming04@126.com
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Abstract

Avian influenza virus is acquiring the ability to cross the species barrier between birds and mammals. However, the genetic mechanism of this ability is not well known. We successfully rescued an influenza A virus A/Chicken/Guangdong/03 (H5N1), which is highly pathogenic to both SPF chickens and BALB/c mice, by plasmid-based reverse genetics. The rescued virus, R-A/Chicken/Guangdong/03 (R-CG), and the wild-type A/Chicken/Guangdong/03 (W-CG) were found to share similar biological properties, such as in titres of 50% egg infectious dose (EID50), 50% tissue culture infectious dose (TCID50) and intravenous pathogenicity index (IPVI). R-CG, like W-CG, is highly pathogenic in mice following natural route infection. Both R-CG and W-CG viruses can be isolated from many organs of mice such as brain, lung, kidney and spleen. As a result, the constructed R-CG reverse genetic system can be used as a tool in the investigation of the molecular mechanism of infection of the mammalian host by avian influenza virus.

Keywords

rescue of virusavian influenza virusH5N1mouse
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 87 - 92
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(6): 831–835

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