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Real-time quantitative PCR detection of Escherichia coli O157:H7

Published online by Cambridge University Press:  15 June 2007

Chen Si ,
Huang Kun-Lun ,
Xu Wen-Tao ,
Li Yuan  and
Luo Yun-Bo
Chen Si
Affiliation:
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Huang Kun-Lun
Affiliation:
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Xu Wen-Tao
Affiliation:
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Li Yuan
Affiliation:
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Luo Yun-Bo*
Affiliation:
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
*
*Corresponding author. E-mail: yunbol@public3.bta.net.cn
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Abstract

A rapid and accurate real-time quantitative polymerase chain reaction (real-time PCR) method with SYBR Green I was established for detecting Escherichia coli O157:H7. A pair of primers were designed to amplify the eae gene. The dissociation curves showed that the amplification product was very specific. The optimal conditions and standard curve were established. The result indicated that real-time PCR was 1000 times more sensitive than ordinary PCR.

Keywords

Escherichia coli O157:H7SYBR Green Iquantitative fluorescencereal-time PCR
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 15 - 19
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(5): 779–782

†

These authors contributed equally.

References

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