Skip to main content Accessibility help

Microsatellite analysis of landrace rice core collection in Yunnan, China

  • Zhang Hong-Liang (a1), Li Zi-Chao (a1), Liao Deng-Qun (a1), Liu Xia (a1) (a2), Zeng Ya-Wen (a3), Shen Shi-Quan (a3), Mu Ping (a1), Yang Zhong-Yi (a3) and Wang Xiang-Kun (a1)...


The distribution of genetic diversity between Oryza sativa L. ssp. indica and O. sativa L. ssp. japonica covering different ecological zones in Yunnan was studied, and specific markers of indica/japonica subspecies, paddy/upland rice and different ecological zones were screened, using 36 microsatellite primers and 113 accessions in the Yunnan landrace rice core collection. The genetic diversity of japonica was higher than that of indica, and the ecological zone with the highest and smallest genetic diversity lay in south-east and north-east Yunnan, respectively. This distribution was consistent at morphological and isozyme levels with studies on the entire Yunnan rice resources and core collection. In addition, the results showed that, among 416 markers, there were six indica/japonica-specific markers, 15 specific markers for paddy/upland rice and three specific markers in different ecological zones. The main conclusions are that the landrace rice core collection in Yunnan genetically represents the entire landrace rice resources in Yunnan, the centre of genetic diversity at DNA level lies in south-east Yunnan, and the DNA differentiation between indica and japonica is small. Furthermore, microsatellite markers were useful for studying the genetic diversity, classification and ecotype of germplasm resources and their core collection.


Corresponding author

*Corresponding author. E-mail:


Hide All
Doyle, JJ and Doyle, JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 1315.
Frankel, OH (1984) Genetic perspectives of germplasm conservation. In: Arber, W et al. ,(editors) Genetic Manipulation: Impact on Man and Society. Cambridge: Cambridge University Press, pp. 161170.
Frankel, OH and Brown, AHD (1984) Current plant genetic resources – a critical appraisal Chopra, VL et al. , (editors) Genetics: New Frontiers, Vol. IV: New Delhi: Oxford and IBH Publishing. 313.
Fu, C-X, Jiang, H and Tan, X-Y (1981) Analysis of rice variety and their evolution using esterase isozyme. Jiangsu Agricultural Science 4: 3135. (in Chinese with English abstract)
Jiang, Z-N and Yan, Y-X (1998) Diversity of rice germplasm resources in Yunnan. Hereditas Supplement 20: 98102. (in Chinese with English abstract)
Li, Y-H, Xiao, H and Zhang, C-C (1999) Genetic variation of main parents of hybrid rice in China was revealed with simple sequence repeat markers. Acta Botanica Sinica 41: 10611066. (in Chinese with English abstract)
Li, Z-C, Zhang, H-L, Sun, C-Q et al. (1999) Status and prospects of core collection in plant germplasm resources. Journal of China Agricultural University 4: 5162. (in Chinese with English abstract)
Li, Z-C, Zhang, H-L, Zeng, Y-W (2000) Study on sampling schemes of core collection of local varieties of rice in Yunnan, China. Scientia Agricultura Sinica 33: 18. (in Chinese with English abstract)
Li, Z-C, Zhang, HL and Zeng, YW et al. ,(2001) Studies on phenotype diversity of rice germplasm in Yunnan, China. Acta Agro Sinica 27: 872–837. (in Chinese).
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the USA 70: 33213323.
Nei, M (1977) F-statistics and analysis of gene diversity in subdivided populations. Annals of Human Genetics 41: 225233.
Panaud, O, Chen, X and McCouch, SR (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice ( Oryza sativa L.). Molecular and General Genetics 252: 597607.
Shen, J-X and Guo, Q-Y (1995) Clustered analysis of sesame germplasm resources in China. Journal of Huazhong Agricultural University 14: 532536. (in Chinese with English abstract)
Sun, X-L, Cai, H-W and Wang, X-K (1996) Diversity of rice genetic resources in Yunnan. Acta Genetica Sinica 23: 276285. (in Chinese with English abstract)
Wang, X-K, Chen, Y-W and Cheng, K-S (1984) A comprehensive study of indigenous rices in Yunnan and their utilization III: the nuda in Yunnan. Journal of Beijing Agricultural University 10: 333343. (in Chinese with English abstract)
Yun, R, Wang, H-X, Hu, Z-A et al. ,(1998) Genetic differentiation of Quercus mongolica and Q. liaotungensis based on morphological observation, isozyme and DNA analysis. Acta Botanica Sinica 40: 10401046. (in Chinese with English abstract)
Zhang, HL (2000) Studies on core collection of landrace rice resources in Yunnan, China. Master thesis, China Agricultural University (in Chinese).
Zhou, Y-H (1988) Regionalization of rice cropping in Yunnan. In: Min, S and Wu, X(editor) Regionalization of Rice Cropping in China. Hangzhou: Zhejiang Science and Technology Publisher pp. 104108.(in Chinese)



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed