Skip to main content Accessibility help

Inter-family distant hybridization of Oryza sativa and Oenothera biennis

  • Chu Xiu-Cheng (a1), Zhao Ji-Hong (a1), Chai Yang (a2), Zhao Jian-Feng (a1), Zhao Yun-Yang (a2), Jiang Li-Yan (a1), Zhao Fen-Shan (a1), Yu Hui-Qin (a1), Zhao Wei-Dong (a1) and You Zhi-Qiang (a1)...


Progenies derived from rice (Oryza sativa ssp. japonica) plants pollinated by Oenothera biennis exhibited numerous morphological and developmental traits. Some variant individuals appeared in generation D2. From generations D3 to D6, a large number of variants were observed, showing distinct variable traits including giant embryos. Statistical analysis on D6 lines showed significant differences between progenies and their rice parental line in several main traits, including plant height (49.2–164.5 cm), panicle number (12.1–38.2), panicle length (20.3–30.3 cm), length of sword leaf (13.8–57.5 cm), leaf width (11.1–25.2 mm), grain number of main panicle (142.0–367.0), percentage of seed setting (0.8–99.0%), 1000-grain weight (19.7–33.8 g) and time from germination to panicle emergence (90.0–108.0 days). Most of the variable coefficients were above 20% (the highest was 40.8%). Through pedigree selection of these plants, genetically stable lines were obtained, which are useful for rice breeding. Results from amplified fragment length polymorphism (AFLP) analysis showed that several rice lines contained extensive genetic variations, which included disappearance of rice parental bands and/or appearance of novel bands.


Corresponding author

*Corresponding author. E-mail:


Hide All
Chu, XC, Zhao, JH, Jiang, LY, et al. (2003) Study on the apomixes and breeding strategies of rice. Journal of Jilin Agricultural Sciences 28(3): 58 (in Chinese).
Duan, XL and Chen, SB (1985) Alien DNA introgression into rice causes trait alterations. Scientia Agricultura Sinica 3: 69 (in Chinese with English abstract).
Liu, B, He, MY and Hao, S (1999a) Production and molecular characterization of rice lines with introgressed traits from a wild species Zizania latifolia (Griseb.). Genetics and Breeding 53: 279284.
Liu, B, Piao, HM, Zhao, FS, Zhao, JH, Liu, ZL and Huang, BQ (1999b) DNA methylation changes in rice induced by Zizania latifolia (Griseb.) DNA introgression. Hereditas 131: 7578.
Liu, B, Dong, YZ, Liu, ZL, He, MY and Wang, YD (2001) Foreign DNA introgression caused heritable cytosine demethylation in ribosomal RNA genes of rice. Acta Physiologiae Plantarum 23: 415420.
Liu, ZL, Wang, YM, Shen, Y, Guo, WL, Hao, S and Liu, B (2004) Alien DNA introgression caused heritable alterations in DNA methylation and transcription in a plant genome. Plant Molecular Biology 54: 7376.
Wang, XL (2006) Advance of researches on Zizania latifolia in rice breeding. Journal of Jilin Agricultural Sciences 31(1): 3536, 49 (in Chinese).
Wang, YM, Dong, ZY, Zhang, ZJ, et al. (2005) Extensive de novo genomic variation in rice induced by introgression from wild rice (Zizania latifolia Griseb.). Genetics 170: 19451956.
Yang, MH (1960) Preliminary observations of rice and Guizhou barnyardgrass hybrid test. Journal of Agriculture 11(1): 7781 (in Chinese with English abstract).
Zhao, JH, Jiang, LY, Chu, XC, Zhao, JF, Wu, ZH and Ren, WJ (2000) Study on variation of characters of rice introduced by exogenous DNA of wild rice. Journal of Northeast Normal University 32(2): 5559 (in Chinese with English abstract).
Zhou, GY (1978) Study on distant hybridization theory in the perspective of biological evolution. Scientia Agricultura Sinica 2: 1620 (in Chinese with English abstract).
Zhu, DM, Dai, LF and Chen, SB (1979) The diversity and specific performance of progenies from distant hybridization between rice and sorghum. Acta Genetica Sinica 6(4): 414420 (in Chinese with English abstract).



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed