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Influence of genetically modified rice containing codA gene on physiological metabolism and genetic horizontal transformation in fed rats

  • Zhao Zhi-Hui (a1) (a2), Yang Li-Tao (a3) (a4), Ai Xiao-Jie (a5), Zhang Da-Bing (a4) and Zou Si-Xiang (a1)...

Abstract

To analyse the influence of genetically modified (GM) rice (Oryza sativa) containing the exogenous choline oxidase (codA) gene on physiological metabolism and genetic horizontal transformation in the fed rats, the animals were divided randomly into two groups. One group was fed with the feedstuff containing 30% of transgenic rice. The remaining (control) group was fed with the feedstuff containing 30% of non-transgenic rice. After 30 days, growth, physiological metabolism parameters such as growth hormone (GH), insulin, glucagon, 3,3′,5-triiodothyronine (T3), thyroxine (T4), thyroid-stimulating hormone (TSH), and the genetic horizontal transformation were analysed. Results indicated that the weight of all rats from the two groups was similar (P>0.05). Also, there was no significant difference in the level of GH, insulin, glucagon, T4, T3 and TSH between the treated and untreated rats. The serum urea nitrogen (SUN), glucose, the glutamate–pyruvate transaminase (GTP), as well as the total cholesterol, of treated rats were almost identical to those of the control (P>0.05). Moreover, no target DNA sequence of rice endogenous sucrose phosphate synthase (SPS) gene or exogenous choline oxidase (codA) gene was detected in organs (liver, muscle, pancreas, etc.) except the stomach, gastrointestinal and rectum contents, and no genetic horizontal transformation was observed in rats fed with transgenic rice.

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Corresponding author

*The first two authors did equivalent work in this study.
**Corresponding author: E-mail: sixiangzou@yahoo.com.cn

References

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Ai, XJ (2005) Effects of crude enzyme preparation supplemented by barley diet on the performance, hormone levels and biochemical parameters in goslings. Journal of the Shanghai Jiao Tong University (Science) E-10 (S1): 115119.
Ai, XJ and Han, ZK (1998) Effect of cysteamine on the plasma content of β-END, IGF I and same hormones related to growth in goslings. Acta Veterinaria et Zootechnica Sinica 29(3): 283288 (in Chinese with English abstract).
Barrière, Y, Verite, R, Brunschwig, P, Surault, F and Emile, JC (2001) Feeding value of corn silage estimated with sheep and dairy cows is not altered by genetic incorporation of Bt176 resistance to Ostrinia nubilalis. Journal of Dairy Science 84: 18631871.
Brake, J and Vlachos, D (1998) Evaluation of transgenic event 176 ‘Bt’ corn in broiler chickens. Poultry Science 77: 648653.
Chowdhury, EH, Kuribara, H, Hino, A et al. , (2003a) Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11. Journal of Dairy Science 81: 25462551.
Chowdhury, EH, Mikami, O, Nakajima, Y et al. , (2003b) Detection of genetically modified maize DNA fragments in the intestinal contents of pigs fed StarLink CBH351. Veterinary and Human Toxicology 45: 9596.
Ding, J, Jia, J, Yang, L, Weng, H, Zhang, C, Liu, W and Zhang, D (2004) Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. Journal of Agricultural and Food Chemistry 52: 33723377.
Einspanier, R, Klotz, A, Kraft, J et al. , (2001) The fate of forage plant DNA in farm animals: A collaborative case-study investigating cattle and chicken fed recombinant plant material. European Food Research and Technology 212: 129134.
He, PM, Zhang, DB, Liang, WQ, Yao, QH and Zhang, RX (2001) Expression of choline oxidase gene (codA) enhances salt tolerance of the tomato. Acta Biochemica et Biophysica Sinica 33: 519524.
Jennings, JC, Albee, LD, Kolwyck, DC et al. , (2003) Attempts to detect transgenic and endogenous plant DNA and transgenic protein in muscle from broilers fed YieldGard Corn Borer Corn. Poultry Science 82: 371380.
Kuiper, HA, Kleter, GA, Noteborn, HP and Esther, JK (2001) Assessment of the food safety issues related to genetically modified foods. Plant Journal 27: 503528.
Miraglia, M, Berdal, KG, Brera, C et al. , (2004) Detection and traceability of genetically modified organisms in the food production chain. Food and Chemical Toxicology 42: 11571180.
Phipps, RH, Deaville, ER and Maddison, BC (2003) Detection of transgenic and endogenous plant DNA in rumen fluid, duodenal digesta, milk, blood, and feces of lactating dairy cows. Journal of Dairy Science 86: 40704078.
Shirai, N, Momma, K, Ozawa, S et al. , (1998) Safety assessment of genetically engineered food: detection and monitoring of glyphosate-tolerant soybeans. Bioscience Biotechnology and Biochemistry 62: 14611464.

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