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In vitro selection of herbicide resistance in microspore-derived embryos of oilseed rape (Brassica napus L.)

  • Xu Ling (a1), Zhang Guo-Qing (a1) (a2), Gu Hong-Hui (a1) (a3), He Yun (a1) and Zhou Wei-Jun (a1)...

Abstract

Using four oilseed rape (Brassica napus L.) F1 hybrids (7039, 7040, 282 and 5102) as donor plants for microspore culture, the experiment was conducted to select glyphosate- and haloxyfop-resistant embryos through application of these substances to the cultural media with microspore-derived embryos in vitro. Genotypes 7039 and 7040 were used to select glyphosate-resistant regenerated plants, and genotypes 282 and 5102 to select haloxyfop-resistant plants. The embryos at cotyledonary stage were grown on glyphosate- and haloxyfop-containing MS-2 medium for 2 weeks. The non-resistant embryos collapsed after a short time, while the resistant ones turned green and survived for 2 weeks. Transferred into the normal MS-2 medium for further plant regeneration, the regenerated plants from green embryos showed tolerance to 0.25% sprayed glyphosate, indicating the effectiveness and reliability of this in vitro selection method. When the regenerated plants selected from 0.02% haloxyfop were sprayed with 0.05% haloxyfop, most of them grew well; however, the survival rate of the regenerated plants from 0.01% haloxyfop-containing medium was lower. The present experiment indicates that the use of 0.02% haloxyfop in the selection of haloxyfop-resistant plants was more promising than that of 0.01%. The chromosome doubling efficiency of regenerated plants reached 34% and 52% after being treated with 170 mg/l colchicine for 20 and 30 h, respectively.

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Corresponding author

*Corresponding author: E-mail: wjzhou@zju.edu.cn

References

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Beversdorf, WD and Hume, DJ (1984) OAC Triton spring rapeseed. Canadian Journal of Plant Science 64: 10071009.
Fletcher, R, Coventry, J and Kott, LS (1998) Doubled haploid technology for spring and winter Brassica napus, revised ed. OAC Publication. University of Guelph, Guelph, Ontario, Canada, pp. 142.
Gu, HH, Zhou, WJ and Hagberg, P (2003a) High frequency spontaneous production of doubled haploid plants from microspore culture in Brassica rapa ssp. chinensis. Euphytica 134: 239245.
Gu, HH, Zhu, JQ, Zhang, GQ and Zhou, WJ (2003b) Microspore culture and ploidy identification of regenerated plant in Chinese flowering cabbage (Brassica rapa ssp. parachinensis). Journal of Agricultural Biotechnology 11: 572576 (in Chinese with English abstract).
Gu, HH, Hagberg, P and Zhou, WJ (2004a) Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.). Plant Growth Regulation 42: 137143.
Gu, HH, Hu, LJ and Zhou, WJ (2004b) Applications of microspore culture technology in Brassica breeding programs. Journal of Agricultural Biotechnology 12: 219223 (in Chinese with English abstract).
Kott, LS (1998) Application of doubled haploid technology in breeding of oilseed Brassica napus. Agriculture and Biotechnology News Information 10: 6974.
Lichter, R (1982) Induction of haploid plants from isolated pollen of Brassica napus L. Zeitschrift für Pflanzenphysiol 105: 427434.
Liu, ZS, Guan, CY and Chen, SY (2003) Research and progress of herbicide-resistance in rapeseed. Crop Research 17: 7072 (in Chinese).
Momoh, EJJ, Zhou, WJ and Kristiansson, B (2002) Variation in the development of secondary dormancy in oilseed rape genotypes under conditions of stress. Weed Research 42: 446455.
Murashige, T and Skoog, F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum 15: 473497.
Palmer, CE, Keller, WA and Arnison, PG (1996) Utilization of Brassica haploids. In: JainSM, SM,, Sopory, SK and Veilleux, RE (editors). In Vitro Haploid Production in Higher Plants, Vol. 3. Dordrecht, Netherlands: Kluwer Academic Publishers, pp. 173192.
Pu, HM (2003) Review of genetically modified rapeseed with herbicide-resistance and ecologic safety. Chinese Journal of Oil Crop Sciences 25: 8993 (in Chinese).
Shi, SW and Zhou, YM (1998) Transformation of Brassica napus with herbicide resistance gene. Journal of Huazhong Agricultural University 17: 205210 (in Chinese with English abstract).
Zhang, FL and Takahata, Y (1999) Microspore mutagenesis and in vitro selection for resistance to soft rot disease in Chinese cabbage (Brassica campestris L. ssp. pekinensis). Breeding Science 49: 161166.
Zhang, GQ, Zhou, WJ, Gu, HH, Song, WJ and Momoh, EJJ (2003) Plant regeneration from the hybridization of Brassica juncea and B. napus through embryo culture. Journal of Agronomy and Crop Science 189: 347350.
Zhang, GQ, Tang, GX, Song, WJ and Zhou, WJ (2004) Resynthesizing Brassica napus from interspecific hybridization between Brassica rapa and B. oleracea through ovary culture. Euphytica 140: 181187.
Zhou, WJ, Hagberg, P and Tang, GX (2002a) Increasing embryogenesis and doubling efficiency by immediate colchicine treatment of isolated microspores in spring Brassica napus. Euphytica 128: 2734.
Zhou, WJ, Mao, BZ, Gu, HH and Tang, GX (2002b) Effects of colchicine with heat shock and cold induction on plant regeneration from microspore-derived embryos in Brassica napus. Acta Agronomica Sinica 28: 369373 (in Chinese with English abstract).
Zhou, WJ, Mao, BZ, Tang, GX and Hagberg, P (2002c) Studies on the determination of ploidy level of microspore-derived plants in Brassica napus. Scientia Agricultura Sinica 35: 724727 (in Chinese with English abstract).
Zhou, WJ, Tang, GX and Hagberg, P (2002d) Efficient production of doubled haploid plants by immediate colchicine treatment of isolated microspores in winter Brassica napus. Plant Growth Regulation 37: 185192.
Zhou, WJ, Yoneyama, K, Takeuchi, Y et al. , (2004) In vitro infection of host roots by differentiated calli of the parasitic plant Orobanche. Journal of Experimental Botany 55: 899907.

Keywords

In vitro selection of herbicide resistance in microspore-derived embryos of oilseed rape (Brassica napus L.)

  • Xu Ling (a1), Zhang Guo-Qing (a1) (a2), Gu Hong-Hui (a1) (a3), He Yun (a1) and Zhou Wei-Jun (a1)...

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