Skip to main content Accessibility help
×
Home

In vitro differentiation of chicken spermatogonial stem cells into adipocytes

  • Yu Fei (a1), Ge Jian-Hui (a1), Ni Li-Gang (a1), He Xian-Hong (a1), Xu Qi (a1) and Li Bi-Chun (a1) (a2)...

Abstract

Spermatogonial stem cells (SSCs), which were isolated from chicken (Gallus gallus) embryo testes 16 days after laying, were cultured, subcultured, and induced into adipocytes in vitro. The differentiated cells were identified by oil red-O staining. Dexamethasone, insulin and 3-isobutyl-1-methylxanthine (IBMX) were tested for their induction potential. About 7–21 days after induction, SSCs differentiated into adipocytes, and the resulting adipocytes strongly expressed peroxisome proliferation activation receptor-γ (PPAR-γ). The assay outcome showed that an optimal treatment consisted of dexamethasone, insulin and IBMX application for 3 days and insulin for 1 day (3 cycles), then insulin for 21 days. The differentiation ratio was 85%, better than the combined use of dexamethasone, insulin and IBMX (P<0.01). However, the combination of the three derivatives triggered a stronger induction than any of them used alone (P<0.01). This study has demonstrated the potential of chicken embryonic SSCs to differentiate in vitro into adipocytes.

Copyright

Corresponding author

*Corresponding author. E-mail: yubcli@yzu.edu.cn

References

Hide All
Cao, J, Jin, Y and Zheng, CX (2002) Isolation and induced multi-differentiation of monkey marrow mesenchymal stem cells. Journal of The Fourth Military Medical University 23(24): 23052308 (in Chinese).
Chen, JJ and London, IM (1981) Hemin enhances the differentiation of mouse 3T3 cells to adipocytes. Cell 26(1): 117122.
El Hadri, K, Pairault, J and Feve, B (1996) Triiodothyronine regulates beta 3-adrenoceptor expression in 3T3-F442A differentiating adipocytes. European Journal of Biochemistry 239(2): 519.
Gagnon, A, Dods, P and Roustan-Delatour, N (2001) Phosphatidylinositol-3,4,5-trisphosphate is required for insulin-like growth factor 1-mediated survival of 3T3-L1 preadipocytes. Endocrinology 142: 205212.
Gregoire, FM, Smas, CM and Sul, HS (1998) Understanding adipocyte differentiation. Physiological Review 78(3): 783790.
Jessen, BA and Tevens, GJ (2002) Expression profiling during adipocyte differentiation of 3T3-L1 fibroblasts. Gene 299(1): 95102.
Klemm, DJ, Roesler, WJ, Boras, T, Colton, LA, Felder, K and Reusch, JE-B (1998) Insulin stimulates cAMP-response element binding protein activity in HepG2 and 3T3-L1 cell line. Journal of Biological Chemistry 273: 917923.
Li, SF, Huang, DQ, Lu, XF, et al. (2003) Study on rabbit mesenchymal stem cells differentiation to the adipogenic or osteogenic lineage in vitro. Journal of Biomedical Engineering 20(2): 209213 (in Chinese).
Lim, JY, Jeun, SS, Lee, KJ, et al. (2006) Multiple stem cell traits of expanded rat bone marrow stromal cells. Experimental Neurology 199(2): 416426.
Naruse, K, Urabe, K, Mukaida, T, et al. (2004) Spontaneous differentiation of mesenchymal stem cells obtained from fetal rat circulation. Bone 35(4): 850858.
Oatley, JM and Brinster, RL (2006) Spermatogonial stem cells. Methods in Enzymology 419: 259282.
Saladin, R, Fajas, L, Dana, S, Halvorsen, Y-D, Auwerx, J and Briggs, M (1999) Differential regulation of peroxisome proliferator activated receptor gamma 1 (PPAR gamma 1) and PPAR gamma 2 messenger RNA expression in the early stages of adipogenesis. Cell Growth and Differentiation 10: 4348.
Schlatt, S (2002) Spermatogonial stem cell preservation and transplantation. Molecular Cell Endocrinology 187(122): 107111.
Tang, QQ, Jiang, MS and Lane, MD (1999) Repressive effect of Sp1 on the C/EBPalpha gene promoter: Role in adipocyte differentiation. Molecular Cell Biology 19: 48554865.
Wu, H, Li, BC, Zhou, GY, Sun, SY and Qin, J (2007) Study on the separation methods and culture of chicken testicular cells. Chinese Journal of Veterinary Medicine 43(3): 1116.
Yamaguchi, T, Chattopadhyay, N and Kifor, O (1998) Extracellular calcium (Ca2+(o))-sensing receptor in a murine bone marrow-derived stromal cell line (ST2): potential mediator of the action of Ca2+(o) on the function of ST2 lines. Endocrinology 139: 35613568.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed