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Genetic diversity and molecular markers of five snapper species

Published online by Cambridge University Press:  15 June 2007

Liu Li  and
Liu Chu-Wu
Liu Li
Affiliation:
Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China
Liu Chu-Wu*
Affiliation:
Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China
*
*Corresponding author. E-mail: swyjs@gdou.edu.cn
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Abstract

In order to protect and develop valuable snappers (Lutjanus spp.), genetic diversity and molecular markers of five species (Lutjanus vitta, L. fulvus, L. fulviflamma, L. sebae and L. stellatus) were detected and analysed using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) techniques. The polymorphic loci ratio (P) (86.00–92.11%), the mean intraspecies genetic distances (D) (0.1775–0.3431) and the intraspecies genetic diversity indexes (Hi) (0.1022–0.1634) were calculated using the RAPD technique. The genetic diversities of L. fulviflamma and L. vitta were richest in terms of P, and D and Hi, respectively. The results of SSR showed that low effective numbers of alleles (1.7893–3.6591), medium average heterozygosities (0.332–0.676) and medium polymorphism information contents (PIC) (0.302–0.641) occurred in five species of snappers, indicating comparatively rich genetic diversity among these fish. Nine molecular markers in the products amplified by primers OPA8 and OPP10, and six molecular markers in 11 microsatellite loci were found to be useful as specific markers to identify five species of snappers. Two neighbour-joining (NJ) dendrograms based on the results of RAPD and SSR suggested that L. stellatus and L. sebae are closely related and clustered in one branch, with L. vitta, L. fulviflamma and L. fulvus in the other.

Keywords

genetic diversityLutjanus vittaL. fulvusL. fulviflammaL. sebaeL. stellatusmolecular markerrandom amplified polymorphic DNAsimple sequence repeats
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 39 - 46
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(3): 349–355

References

Allman, RJ and Churchill, BG (2002) Temporal and spatial dynamics of spawning, and growth of gray snapper (Lutjanus griseus) from the West Florida shelf as determined from otolith microstructures. Fishery Bulletin 100(3): 391–403.Google Scholar
Allman, RJ and Lombardi-Carlson, LA (2000) Age structure of red snapper (Lutjanus campechanus) in the Gulf of Mexico by fishing mode and region. Proceedings of the Gulf and Caribbean Fisheries Institute 53: 482–495.Google Scholar
Bardakci, F and Skibinski, DOF (1994) Application of the RAPD technique in tilapia fish: species and subspecies identification. Heredity 73: 117–123.CrossRefGoogle ScholarPubMed
Botsein, D, White, RL, Skolnick, M, et al. (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32: 314–331.Google Scholar
Cao, FJ, Li, CL and Liu, CW (2002) Studies on the karyotypes of Lutjanus erythopterus, Lutjanus argetimaculatus and Lutjanus bohar. Marine Sciences 26(11): 43–46 (in Chinese with English abstract).Google Scholar
Chen, G (1997) Studies on age, growth and life-history pattern of Lutjanus russelli Bleeker. Journal of Fisheries of China 21(1): 6–12 (in Chinese with English abstract).Google Scholar
Chen, JF, Zhuang, FY, Lu, MH, Qian, CT and Ren, G (2003) Phylogenetic relationships in cucumis (Cucurbitaceae) revealed by SSR and RAPD analyses. Acta Phytotaxonomica Sinica 41(5): 427–435 (in Chinese with English abstract).Google Scholar
Chow, S, Clarke, ME and Walsh, PJ (1993) PCR-RFLP analysis on thirteen western Atlantic snappers (subfamily Lutjaninae): A simple method for species and stock identification. Fishery Bulletin US 91: 619–627.Google Scholar
Collins, LA, Fitzhugh, GR, Mourand, L, et al. (2001) Preliminary results from a continued study of spawning and fecundity in the red snapper (Lutjanidae: Lutjanus campechanus) from the Gulf of Mexico, 1998–1999. Proceedings of the Gulf and Caribbean Fisheries Institute 52: 34–47.Google Scholar
Ding, SX, Wang, J, Quan, CG and Su, YQ (1998) Gentic diversity in population of cultured Nibea miichthioides. Bulletin of Science 43(21): 2294–2299 (in Chinese).Google Scholar
Heist, EJ (2000) DNA microsatellite loci and genetic structure of red snapper in the Gulf of Mexico. Transactions of the American Fisheries Society 129(2): 469–475.2.0.CO;2>CrossRefGoogle Scholar
Kumar, S, Tamura, K, Jacobsen, IB and Nei, M (2001) MEGAZ: molecular evolutionary genetics analysis software. Bioinformatics 17: 1244–1245.CrossRefGoogle Scholar
Li, CL, Cao, FJ, Liu, CW and Chen, G (2001) Microstructure of peripheral blood cells in Lutjanus russelli. Journal of Zhanjing Ocean University 21(3): 5–9 (in Chinese with English abstract).Google Scholar
Lin, KD and Luo, C (2003) Preliminary study on applicability of microsatellite primers developed from common carp for genomic analysis of grass carp. Acta Laser Biology Sinica 12(2): 121–127 (in Chinese with English abstract).Google Scholar
Liu, L, Liu, CW and Xu, Q (2003a) Population genetics analysis on three species of snappers by random amplified polymorphic DNA (RAPD). Journal of Zhanjing Ocean University 23(3): 16–21 (in Chinese with English abstract).Google Scholar
Liu, L, Liu, CW and Xu, Q (2003b) Population genetics analysis on two species of snappers by random amplified polymorphic DNA (RAPD). Marine Sciences 27(10): 59–62 (in Chinese with English abstract).Google Scholar
Liu, ZH, Shi, T, Liu, XZ, Chen, C and Kong, J (1999) Identification of three populations (Chinese Fugu rubripes, Japanese Fugu rubripes and Fugu pseudommus) using RAPD markers. Transactions of Oceanology and Limnology 4: 38–41 (in Chinese).Google Scholar
Liu, ZJ, Li, P, Argue, BJ and Dunham, RA (1999) Random amplified polymorphic DNA markers: usefulness for gene mapping and analysis of genetic variation of catfish. Aquaculture 174: 59–68.CrossRefGoogle Scholar
Lu, SD (1993) Current Protocols for Molecular Biology. Beijing: China Higher Education Press, pp. 95–1045 (in Chinese).Google Scholar
Lynch, M (1990) The similarity index and DNA fingerprinting. Molecular Biology and Evolution 7: 478–484 (in Chinese with English abstract).Google ScholarPubMed
Meng, QW, Su, JX and Miao, XZ (1995) Systematics of Fishes. Beijing: China Agriculture Press, pp. 686–687 (in Chinese).Google Scholar
Nei, M (1972) Genetic distance between populations. American Naturalist 106: 283–292.CrossRefGoogle Scholar
Nei, M and Li, WH (1979) Mathematical model for studying genetic variation in terms of restriction endonuclease. Proceedings of the National Academy of Sciences of the USA 76(10): 5269–5273.CrossRefGoogle Scholar
Nielsen, JL, Gan, CA, Wright, JM, Morris, DB and Thomas, WK (1994) Biogeographic distributions of mitochondrial and nuclear markers for southern steelhead. Molecular Marine Biology and Biotechnology 3: 281–293.Google Scholar
Reilly, A, Elliott, NG, Grewe, PM, Clabby, C, Powell, R and Ward, RD (1999) Genetic differentiation between Tasmanian cultured Atlantic salmon (Salmo salar L.) and their ancestral Canadian population: comparison of microsatellite DNA and allozyme and mitochondrial DNA variation. Aquaculture 173: 459–469.CrossRefGoogle Scholar
Sambrook, J, Fritsch, EF and Maniatis, T (1989) Molecular Cloning: a Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.Google Scholar
Yi, LF and Liu, CW (2005) RAPD analysis of four snapper species. Fisheries Science 24(1): 9–12 (in Chinese with English abstract).Google Scholar
Yi, LF, Liu, CW and Lu, LQ (2002) RAPD analysis on genetic diversity of Lutjanus johni. Journal of Fishery Sciences of China 9(4): 379–381 (in Chinese with English abstract).Google Scholar
Yoon, JM and Kim, GW (2001) Randomly amplified polymorphic DNA-polymerase chain reaction analysis of two different populations of cultured Korean catfish Silurus asotus. Journal of Biosciences 26(5): 641–647 (in Chinese with English abstract).CrossRefGoogle ScholarPubMed
Zhou, Fl, Jiang, SG, Su, TF and Lv, JL (2004a) Comparative study of mtDNA 16S rRNA gene fragments among six Lutjanus fishes. Journal of Fishery Sciences of China 11(2): 99–103 (in Chinese with English abstract).Google Scholar
Zhou, Fl, Jiang, SG, Su, TF and Lv, JL (2004b) Comparison of mitochondrial cytochrome b gene fragments among six species of Lutjanus. Journal of Tropical Oceanography 23(4): 87–92 (in Chinese with English abstract).Google Scholar
Zhu, LF, Zhang, XL and Nie, YC (2003) Analysis of genetic diversity in upland cotton (Gossypium hirsutum L.) cultivars from foreign countries by RAPDs and SSRs. Journal of Agricultural Biotechnology 11(5): 450–455 (in Chinese with English abstract).Google Scholar