Skip to main content Accessibility help
×
Home

Expressing activator protein Ap36 in Bacillus thuringiensis and the function of the recombined strain on disease resistance

  • Peng Dong-Hai (a1) (a2), Zhou Chen-Fei (a1) (a2), Qiu De-Wen (a3), Zhou Kang (a1), Ruan Li-Fang (a1), Chen Shou-Wen (a1), Yu Zi-Niu (a1) and Sun Ming (a1)...

Abstract

The gene ap36 encoding a protein elicitor from Alternaria sp. was fused downstream of the slh (S-layer homology) motif of Bacillus thuringiensis S-layer protein gene ctc. The recombinant gene was then transferred into B. thuringiensis plasmid-free derivative strain BMB171. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the SLH–Ap36 fusion protein was expressed in B. thuringiensis BMB171. After tomato (Lycopersicum esculentum) leaves were treated for 90 min with the recombinant strain cultured at 28°C for 24 h, the activity of peroxidase and the amount of proline of tomato leaves were increased to 57.14% and 131.59%, respectively, compared to the control, and after the tomato leaves were treated with the cultured recombinant strain for 4 days, the activity of phenylalanine ammonia lyase was also higher than that in the control. Furthermore, tubers of treated potato (Solanum tuberosum) plants showed higher resistance to rot disease caused by Erwinia corotovora SCG1 compared to the control treatments.

Copyright

Corresponding author

*Corresponding author. E-mail: m98sun@mail.hzau.edu.cn

References

Hide All
Chen, M, Qiu, DW, Liu, Z, Yang, XF and Cao, KQ (2006) Inhibition of plant activator protein on RNA replication and coat protein synthesis of Tobacco mosaic virus. Chinese Journal of Biological Control 22: 6366.
Hammerschmidt, R, Nuckles, EM and Kuc, J (1982) Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiology and Molecular Plant Pathology 20: 7382.
Kalman, S, Keehne, KL, Cooper, N, Reynoso, MS and Yamamoto, T (1995) Enhanced production of insecticidal protein in Bacillus thuringiensis strain carrying an additional crystal protein gene in their chromosome. Applied and Environmental Microbiology 61: 30633068.
Laemmli, UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680685.
Liu, M, Lu, LJ, Huang, JY, Zhang, SH, Bi, DR and Sun, M (2007) Display of Avian influenza virus of H5N1 haemagglutinin HA1 on Bacillus thuringiensis cell surface and its immunogenicity for mice. Journal of Agricultural Biotechnology 15: 371377.
Lowry, OH, Rosebrough, NJ, Farr, AL and Randall, RJ (1951) Protein measuement with the folin phenol reagent. Journal of Biological Chemistry 193: 256275.
Mauch, MB and Slusarenko, AJ (1996) Production of salicylic acid precursors is a major function of phenylalanine ammonia-lyase in the resistance of Arabidopsis to Peronospora parasitica. Plant Cell 8: 203212.
Mesnage, S, Weber-Levy, M, Haustant, M, Mock, M and Fouet, A (1999) Cell surface-exposed tetanus toxin fragment C produced by recombinant Bacillus anthracis protects against tetanus toxin. Infection and Immunity 67: 48474850.
Pellegrini, L, Rohfritsch, O, Fritig, B and Legrand, M (1994) Phenylalanine ammonia-lyase in tobacco. Plant Physiology 106: 877886.
Qiu, DW (2004) Microbe protein pesticide and its prospect. Chinese Journal of Biological Control 20: 9194.
Qiu, DW, Xiao, YL, Yao, Q, Li, L and Liu, Z (2005) Effect of activator protein on cucumber growth and the activities of dehydrogenase, peroxidase and phenylalanine ammonia lyase. Chinese Journal of Biological Control 21: 4144.
Sambrook, J, Fritsch, EF and Maniatis, T (2002) Molecular cloning: a laboratory manual, 3rd ed. New York: Cold Spring Harbor Laboratory Press.
Sara, M and Selytr, UB (2000) S-layer proteins. Journal of Bacteriology 182: 859868.
Schnepf, E, Crickmore, N, Van Rie, J, et al. (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology and Molecular Biology Reviews 62: 775806.
Sticher, L, Mauch-Mani, B and Metraux, JP (1997) Systemic acquired resistance. Annual Review of Phytopathology 35: 235270.
Tang, XR and Wu, M (2006) Effects of application of zinc, iron and lanthanum on contents of aroma in brown rice and proline in flag leaf of aromatic rice. Hybrid Rice 21: 6972.
Wang, L, Sun, M and Yu, ZN (2004) Capacity of Bacillus thuringiensis S-layer protein displaying polyhistidine peptides on the cell surface. Applied Biochemistry and Biotechnology 119: 133143.
Wu, HG, Liu, X, Sun, M and Yu, ZN (2004) Cell surface display of α-amylase and metallothionein by using Slayer protein of strain CTC. Acta Microbiologica Sinica 44: 658662.
Wu, L, Sun, M and Yu, ZN (2004) A new resolution vector with cry1Ac10 gene based on Bacillus thuringiensis transposon Tn4430. Acta Microbiologica Sinica 40: 264269.
Xue, YL, Ouyang, GC and Ao, SG (1993) Studies on plant phenyalanine ammonia lyase. Acta Phytophysiologica Sinica 9: 301305.
Yang, G, Guan, X, Wang, PG, Wei, H and You, MS (2007) Advances on induced plant defense genes against insect pests. Journal of Agricultural Biotechnology 15: 157166.
Zhu, CG, Sun, M and Yu, ZN (2003) Expression of gene aiiA carrying the promoter of gene cry3Aa in Bacillus thuringiensis. Chinese Journal of Biotechnology 19: 397401.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed