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Effect of some factors on the fusion rate of bovine–rabbit interspecies reconstructed eggs

  • Zhang Xiao-Rong (a1) (a2), Liu Ya (a2), Zhang Yun-Hai (a3), Chen Da-Yuan (a1), Lian Li (a1), Li Jin-Song (a1), Jin Ren-Tao (a2), Zhang Zhi-Guo (a2), Zhang Mei-Ling (a2) and Wang Cun-Li (a2)...

Abstract

Tests were conducted to highlight the effects of donor cell treatment (bovine G0- and non-G0 phase somatic cells refrigerated for 1–5 or 12–14 days at 4°C), fusion solution (with or without cytochalasin B) and age of the rabbit (young, 3–5-months old; or multiparous, 10–12 months old) as oocyte donor on the fusion and mortality rates of bovine–rabbit interspecies reconstructed eggs. Results showed that there were no significant differences (P>0.05) in fusion and mortality rates of eggs reconstructed with G0 cells or non-G0 cells as nuclear donor somatic cells. The same was also true when nuclear donor somatic cells (G0 cells or non-G0 cells) were treated at low temperature (4°C) for 1–5 or 12–14 days. The fusion rate of reconstructed eggs did not change significantly (P>0.05) when adding cytochalasin B into the fusion solution, but the mortality rate increased significantly (P<0.05). No significant effect (P>0.05) was found in fusion rate of the eggs reconstructed with young rabbits' and multiparous rabbits' oocytes as recipient, but the mortality rate decreased significantly (P<0.01) for young rabbit oocytes compared with multiparous rabbit ones.

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*Corresponding author: E-mail: zhxiaorong88@163.com or zhxirong88@sina.com

References

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Cibelli, JB, Stice, SL & Golueke, PJ (1998) Cloned pigs produced by nuclear transfer from adult somatic cells. Science 280, 12561258.
Chen, D-Y, Sun, Q-Y, Liu, J-L et al. (1999) The giant panda ( Ailuropoda melanoleuca) somatic cell nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg. Science in China 42, 346353.
Chen, D-Y, Wen, D-C & Zhang, Y-P et al. (2002) Interspecies implantation and mitochondria fate of panda–rabbit cloned embryos. Biology of Reproduction 67, 637642.
Chesne, P, Adenot, PG & Viglietta, C et al. (2002) Cloned rabbits produced by nuclear transfer from adult somatic cells. Nature Biotechnology 20, 366369.
Dominko, T, Mitalipova, M & Haley, B et al. (1999) Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species. Biology of Reproduction 60, 14961502.
Gauthier, M, Pierson, J & Drolet, M et al. (2001) Sexual maturation and fertility of male Nigerian dwarf goat ( Capra hircus ) clones produced by somatic cell nuclear transfer. Cloning and Stem Cells 3, 151155.
Hosaka, K, Ohi, S & Anoo, A et al. (2000) Cloned mice derived from somatic cell nuclei. Human Cell 13, 237242.
Kuhholzer, B, Hawley, RJ & Lai, L et al. (2001) Clonal lines of transgenic fibroblast cells derived from the same fetus result in different development when used for nuclear transfer in pigs. Biology of Reproduction 64, 16951698.
Liu, J-L, Wang, M-K & Chen, D-Y et al. (2000) Effect of telophase enucleation on bovine somatic nuclear transfer. Theriogenology 54, 989998.
Loi, P, Ptak, G & Barboni, B et al. (2001) Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nature Biotechnology 19, 962964.
Shin, T, Kraemer, D & Pryor, J et al. (2002) A cat cloned by nuclear transplantation. Nature 415, 859.
Wells, DN, Misica, PM & Tervit, HR et al. (1998) Adult somatic cell nuclear transfer is used to preserve the last surviving cow of the Enderby Island cattle breed. Reproduction, Fertility, and Development 10, 369378.
Wilmut, I, Schnieke, AE & McWhir, J et al. (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810813.
Yang, X, Kubota, C & Suzuki, H et al. (1998) Control of oocyte maturation in cow?–?biological factors. Theriogenology 49, 471482

Keywords

Effect of some factors on the fusion rate of bovine–rabbit interspecies reconstructed eggs

  • Zhang Xiao-Rong (a1) (a2), Liu Ya (a2), Zhang Yun-Hai (a3), Chen Da-Yuan (a1), Lian Li (a1), Li Jin-Song (a1), Jin Ren-Tao (a2), Zhang Zhi-Guo (a2), Zhang Mei-Ling (a2) and Wang Cun-Li (a2)...

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