Hostname: page-component-848d4c4894-xfwgj Total loading time: 0 Render date: 2024-06-24T13:01:14.369Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of nanoparticles on the bacterial community of the cucumber phyllosphere

Published online by Cambridge University Press:  30 October 2009

Chang Li-Yan ,
Wang Qi  and
Mei Ru-Hong
Chang Li-Yan
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100193, China
Wang Qi*
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100193, China
Mei Ru-Hong
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100193, China
*
*Corresponding author. E-mail: wangqi@cau.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

With the rapid development of nanotechnology, the security of nanomaterials has been an increasing cause for concern. In this study, the impact of titanium dioxide nanoparticles (nano-TiO2) on the phyllosphere bacterial community were analysed by both a culturable-dependent method and a polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) method. The quantity of culturable phyllosphere bacteria was significantly reduced with an increased concentration of nano-TiO2. With increasing concentrations from 0.002 to 20 mg/ml of nano-TiO2, the quantity of culturable phyllosphere bacteria decreased from 1.8×106 to 3.1×105 cfu/g. The phyllosphere bacteria community was analysed by PCR-DGGE, and when the concentrations of nano-TiO2 were higher than 0.02 mg/ml, the DGGE bands were significantly lower than in the control. Sequencing results of the bands from the DGGE gel showed that there were at least seven genera in the phyllosphere bacteria. Only one uncultured bacterium was unaffected by the concentration of nano-TiO2.

Keywords

nanoparticlesphyllosphere bacteriaDGGE16S rDNAsequencing
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 2 , August 2009 , pp. 141 - 145
Copyright
Copyright © China Agricultural University 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Amézaga-Madrid, P, Silveyra-Morales, R, Córdoba-Fierro, L, et al. (2003) TEM evidence of ultrastructural alteration on Pseudomonas aeruginosa by photocatalytic TiO2 thin films. Journal of Photochemistry and Photobiology B: Biology 70: 45–50.CrossRefGoogle ScholarPubMed
Brumfiel, G (2003) Nanotechnology: a little knowledge. Nature 424: 246–248.CrossRefGoogle ScholarPubMed
Cheng, XY, Wang, LG, Li, L and Xiong, YY (2005) Inhibition of TiO2 against CoUetotrichum gloeosporioides. Chinese Journal of Biological Control 21: 269–272.Google Scholar
Chen, N and Cheng, YQ (2005) Recent progress of nano-TiO2 photocatalysis in antibacterial application. Chemical Industry and Engineering 22: 445–449.Google Scholar
Cho, M, Chung, H and Choi, W (2005) Different inactivation behaviors of MS-2 phage and Escherichia coli in TiO2 photocatalytic disinfection. Applied and Environmental Microbiology 71: 270–275.CrossRefGoogle ScholarPubMed
Guo, SY and Sheng, XC (2004) Ultrastructural studies on photocatalytical sterilization using titanium dioxide nanoparticles. Journal of Chinese Electron Microscopy Society 23: 107–111.Google Scholar
Hirakawa, K, Mori, M, Yoshida, M, Oikawa, S and Kawanishi, S (2004) Photo-irradiated titanium dioxide catalyzes site specific DNA damage via generation of hydrogen peroxide. Free Radical Research 38: 439–447.CrossRefGoogle ScholarPubMed
Jia, G, Wang, HF and Zhao, YL (2005) Biological activities of water-soluble carbon nanomaterials. Environmental Science & Technology 39: 1378.CrossRefGoogle Scholar
Mills, A and Lee, SK (2002) A web-based overview of semiconductor photochemistry-based current commercial applications. Photochemistry and Photobiology A: Chemistry 152: 233–247.CrossRefGoogle Scholar
Monier, JM and Lindow, SE (2005) Aggregates of resident bacteria facilitate survival of immigrant bacteria on leaf surfaces. Microbial Ecology 49: 343–352.CrossRefGoogle ScholarPubMed
Monier, JM and Lindow, SE (2003) Pseudomonas syringae responds to the environment on leaves by cell size reduction. Phytopathology 93: 1209–1216.CrossRefGoogle Scholar
Oberdörster, E (2004) Manufactured nanomaterials (Fullerenes, C60) induce oxidative stress in the brain of juvenile largemouth bass. Environmental Health Perspectives 112: 1058–1062.CrossRefGoogle ScholarPubMed
Øvreås, L, Forney, L, Daae, FL and Torsvik, V (1997) Distribution of bacterioplankton in lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA. Applied and Environmental Microbiology 63: 3367–3373.CrossRefGoogle ScholarPubMed
Rahman, Q, Lohani, M and Dopp, E (2002) Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts. Environmental Health Perspectives 110: 797–800.CrossRefGoogle ScholarPubMed
Sambrook, J and Russell, DW (2001) Molecular Cloning: A Laboratory Manual, 3rd ed.Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.Google Scholar
Service, RF (2003) Nanomaterials show signs of toxicity. Science 300: 243.CrossRefGoogle ScholarPubMed
Service, RF (2004) Nanotechnology grows up. Science 304: 1732–1734.CrossRefGoogle ScholarPubMed
Ye, SY, Liu, SL and He, MS (2003) Preliminary study on technology of disinfection and preservation by photocatalysis using nanometer TiO2 and flashlight. Transactions of the Chinese Society of Agricultural Engineering 19: 180–183.Google Scholar
Zhang, QS, Song, ZH and Zhang, ZK (2002) Photocatalytic effect of nanometer TiO2 and its application in the environmental field. Journal of Qingdao Institute of Chemical Technology 23: 19–23.Google Scholar
Zhao, YL and Chai, ZF (2005) Status of studying bio-environmental activities of nanoscale materials. China Academic Journal Electronic Publishing House 20: 194–199.Google Scholar
Zhao, YL, Zhao, F and Ye, C (2005) Bio-environmental health sciences of nanoscale materials and nanosafety. China Basic Science 2005(2): 19–23.Google Scholar