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Construction of antagonistic, insecticidal, and endophytic strains of Bacillus spp. by protoplast fusion

  • Yan Nian-Long (a1), Qiu Si-Xin (a2), He Hong (a3), Guan Xiong (a1) and Hu Fang-Ping (a2)...


A multifunctional-engineering strain was constructed by fusing the insecticidal bacterium (Bacillus thuringiensis) with vip3A gene and the endophytic bacterium (B. subtilis) protoplasts. Results of polymerase chain reaction (PCR) showed that the insecticidal vip3A gene of B. thuringiensis was present in the fusant. The fusant's antagonism to Fusarium oxysporum f. sp. cucumerinum, the adjusted mortality to Plutella xylostella and the endophytic characters were detected. The result showed that the antagonism of the fusant was against the F. oxysporum f. sp. cucumerinum; the adjusted mortality to P. xylostella reached 59% and it had insecticidal characteristics.


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