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Cloning and expression analysis of porcine small adipocyte factor 1 (SMAF1) gene

  • Yang Hong-Wen (a1), Zheng Rong (a1), Li Feng-E (a1) and Jiang Si-Wen (a1)


cDNA of the porcine small adipocyte factor 1 (SMAF1) gene was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) with degenerate primers designed according to the conserved sequences between human and mouse genes. The cDNA (GenBank accession No. DQ191892) containing a complete encoding region was 256 bp in length, sharing 86 and 78% identity with that of human and mouse, respectively. Comparison of the deduced amino acid sequence between porcine SMAF1 and the human, mouse, cattle and rat protein showed that the amino acid similarity was 81, 67, 84 and 70%, respectively. The results of semi-quantitative RT-PCR showed that the porcine gene was expressed abundantly in adipose tissue, and at a significantly lower level in lean-type pigs (Large White pigs) than in lard-type pigs (Meishan pigs) at the age of 4 months (P<0.05). The results suggest that the porcine SMAF1 gene may have similar functions as in other species, that is, it may regulate adipogenesis and/or adipocyte function.


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Bjorbaek, C and Kahn, BB (2004) Leptin signaling in the central nervous system and the periphery. Recent Progress in Hormone Research 59: 305331.
Burton, GR and McGehee, RE Jr (2004) Identification of candidate genes involved in the regulation of adipocyte differentiation using microarray-based gene expression profiling. Nutrition 20(1): 109114.
Hileman, SM, Pierroz, DD and Flier, JS (2000) Leptin, nutrition and reproduction: Timing is everything. Journal of Clinical Endocrinology and Metabolism 85: 804807.
Hong, YH, Hishikawa, D, Miyahara, H, et al. (2005) Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis. Molecular and Cellular Biochemistry 276: 133141.
Kanehara, H, Suzuki, J, Zenimaru, Y, et al. (2004) Function of hormone-sensitive lipase in diacylglycerol-protein kinase C pathway. Diabetes Research and Clinical Practice 65(3): 209215.
Kershaw, EE and Flier, JS (2004) Adipose tissue as an endocrine organ. Journal of Clinical Endocrinology and Metabolism 89: 25482556.
Kim, JY, Tillison, K and Smas, CM (2005) Cloning, expression, and differentiation-dependent regulation of SMAF1 in adipogenesis. Biochemical and Biophysical Research Communications 326: 3644.
La Cour, T, Gupta, R, Rapacki, K, Skriver, K, Poulsen, FM and Brunak, S (2003) NESbase version 1.0: a database of nuclear export signals. Nucleic Acids Research 31: 393396.
Melcher, K (2000) The strength of acidic activation domains correlates with their affinity for both transcriptional and non-transcriptional proteins. Journal of Molecular Biology 301: 10971112.
Mueller, E, Drori, S, Aiyer, A, et al. (2002) Genetic analysis of adipogenesis through peroxisome proliferators activated receptor gamma isoforms. Journal of Biological Chemistry 277: 4192541930.
Rosen, ED and Spiegelman, BM (2000) Peroxisome proliferator-activated receptor gamma ligands and atherosclerosis: ending the heartache. Journal of Clinical Investigation 106(5): 629631.
Rosen, ED and Spiegelman, BM (2001) PPARgamma: A nuclear regulator of metabolism, differentiation, and cell growth. Journal of Biology and Chemistry 276(41): 3773137734.
Sekiya, M, Osuga, J, Okazaki, H, et al. (2004) Absence of hormone-sensitive lipase inhibits obesity and adipogenesis in Lep ob/ob mice. Journal of Biological Chemistry 279(15): 1508415090.
Soukas, A, Socci, ND, Saatkamp, BD, Novelli, S and Friedman, JM (2001) Distinct transcriptional profiles of adipogenesis and in vitro. Journal of Biological Chemistry 276(36): 3416734174.
Tominaga, K, Kondo, C, Johmura, Y, Nishizuka, M and Imagawa, M (2004) The novel gene fad104, containing a fibronectin type III domain, has a significant role in adipogenesis. FEBS Letters 577(1–2): 4954.
Yeaman, SJ (2004) Hormone-sensitive lipase – new roles for an old enzyme. Biochemical Journal 379(Pt 1): 1122.



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