Skip to main content Accessibility help
×
Home

Analysis of restriction sites and Southern blotting of two molecular markers linked to grape seedless gene

  • Yang Ke-Qiang (a1) (a2), Wang Yue-Jin (a1), Zhang Jin-Jin (a1) (a3), Wang Xi-Ping (a1), Wan Yi-Zhen (a1) and Zhang Jian-Xia (a1)...

Abstract

The cloning and sequencing of two specific molecular markers linked to grape (Vitis vinifera) seedless gene indicated that the full lengths of the markers, 39970524-5-564 (GenBank accession number: AY327513) and 39970524-6-1538 (GenBank accession number: AY327514), were 564 bp and 1538 bp, respectively. The restriction sites of sequences 39970524-5-564 and 39970524-6-1538 were analysed with Wingene231 software. The results indicated that there were 29 and 130 restriction sites in 39970524-5-564 and 39970524-6-1538, respectively, digested by restriction enzymes with recognition sites of six or more than six bases. There was no cleavage site in 39970524-5-564 and there was one site at 135 bp of 39970524-6-1538 when these two markers were digested by EcoRI. Both markers can not be digested by HindIII. Therefore, the full length of 39970524-5-564 and of the two bands of 39970524-6-1538 (1400 bp and 135 bp) could be cut out from the pGEMR-T Easy Vector by EcoRI. The labelled DNA of 39970524-5-564, used as a probe for Southern blotting with genomic DNA from Red Globe, Flame Seedless, Thompson Seedless, four seedless hybrids and three seeded hybrids, showed a unique hybrid band present in all seedless individuals, but absent in all seeded individuals. It was further indicated that the specific marker 39970524-5-564 was derived from the genome of seedless grapes.

Copyright

Corresponding author

*Corresponding author: Email: wangyj@public.xa.sn.cn

References

Hide All
Clark, MS (1998) Plant Molecular Biotechnology–Experimental Manual (translated by Gu HY and Ju LJ). Beijing: High Education Press, pp. 1342 (in Chinese).
Lahogue, F, This, P and Bouquet, A (1998) Identification of a codominant SCAR marker linked to the seedlessness character in grapevine. Theoretical and Applied Genetics 97: 950959.
Li, DB and Xu, P (1994) Method and theory of recombinant DNA. Hangzhou: Science and Technology Press, pp. 6983 (in Chinese).
Striem, MJ, Ben-Hayyim, G and Spiegel, RP (1996) Identifying molecular genetic markers associated with seedlessness in grape. Journal of the American Society of Horticultural Science 121: 758763.
Wang, YJ and Lamikanra, O (1997) Analysis of sequencing the RAPD marker linked to seedless gene in grape. Journal of Northwest Agricultural University 25(4): 15 (in Chinese with English abstract).
Wang, YJ and Lamikanra, O (2002) Application and synthesis on the DNA probe for detecting seedless genes in grape vine. Journal of Northwest Sci-tech University of Agricultural and Forestry (Natural Science Edition) 30(3): 4246 (in Chinese with English abstract).
Wang, YJ, Lamikanra, O, Lu, J and Ramming, DW (1996) Identification of genetic marker linked to seedless genes in grapes using RAPD. Journal of Northwest Agricultural University 24(5): 110 (in Chinese with English abstract).
Wang, YJ, Lamikanra, O and Schell, L (1997) Identification of hybrids derived from Vitis rotundifolia and V. vinifera by RAPD analysis. Journal of Northwest Agricultural University 25(3): 1620 (in Chinese with English abstract).

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed