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Activation tagging library construction and mutant phenotype analysis

Published online by Cambridge University Press:  03 March 2009

Cao Dong-Mei ,
Fan Xi-Ying ,
Wang Yun-Shan  and
Kang Li-Fang
Cao Dong-Mei*
Affiliation:
Institute of Horticulture, Shanxi Agriculture Academy of Science, Taiyuan 030031, China Institute of Botany, Chinese Academy of Science, Beijing 100093, China
Fan Xi-Ying
Affiliation:
Institute of Botany, Chinese Academy of Science, Beijing 100093, China
Wang Yun-Shan
Affiliation:
Institute of Horticulture, Shanxi Agriculture Academy of Science, Taiyuan 030031, China
Kang Li-Fang
Affiliation:
Institute of Horticulture, Shanxi Agriculture Academy of Science, Taiyuan 030031, China
*
*Corresponding author. E-mail: caodm787@sohu.com
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Abstract

Activation tagging plays an important role in plant genomics studies. In this paper, an activation tagging library containing 50 000 Basta-resistant lines was constructed by mediated transformation of the mutant bzr1-D of Aribidopsis thaliana. Among these transformants, 47 lines showed obvious phenotypes, including late flowering time, dwarf growth habit, changed leaf shape, longer leaf petiole, leaves lacking trichomes, sterility and no kink between stem/leaf. Some T-DNA flanking sequences were obtained by TAIL (thermal asymmetric interlaced) and nested PCR. Results showed that the mutants contained one to three T-DNA insertions. The insertions were distributed in the first, fourth and fifth chromosomes of the genome.

Keywords

activation taggingphenotypeT-DNA flanking sequence
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 3 , December 2008 , pp. 217 - 221
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(2): 292–298

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