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Spatio-temporal expression analysis of two kinds of chemical communication-related proteins in the worker bee Apis cerana cerana Fabricius (Hymenoptera: Apidae)

Published online by Cambridge University Press:  30 October 2009

Li Hong-Liang
Affiliation:
College of Life Science, China Jiliang University, Hangzhou 310018, China Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China
Zhang Ya-Li
Affiliation:
Research Center of Biomedicine and Health, Hangzhou 310000, China
Wang Hai-Yan
Affiliation:
Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China
Gao Qi-Kang*
Affiliation:
Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China
Cheng Jia-An
Affiliation:
Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China
*
*Corresponding author. E-mail: qkgao@zju.edu.cn

Abstract

The spatio-temporal expressed profiles of two kinds of chemical communication-related protein genes, the odorant-binding protein of Ac-ASP2 and chemosensory protein of Ac-ASP3, were identified by real-time polymerase chain reaction (PCR). Results obtained using the 2−ΔΔCt method showed that Ac-ASP2 was a gene coding antenna-specific protein that did not express in larvae and pupae, but had discontinuous high abundance periods at 1, 9, 15, 27 and 30 days. The expressing abundance at such periods was at least ten times higher than that at other periods. From the distribution of Ac-ASP3 mRNA observed in different tissues, the transcript levels seemed to be higher in the wings, abdomen and thorax (of order ~106), and lower in the legs, antennae and head (of order ~105). From highest to lowest, the original copy number was found in the various body parts in the following order: wings, abdomen, thorax, legs, antenna, and head. The results suggest that Ac-ASP3 has an intimate relation with the chemosensory behaviour of wings and abdomen in Apis cerana cerana.

Type
Research Papers
Copyright
Copyright © China Agricultural University 2009

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References

Chantawannakul, P, Ward, L, Boonham, N and Brown, M (2006) A scientific note on the detection of honeybee viruses using real-time PCR (TaqMan) in Varroa mites collected from a Thai honeybee (Apis mellifera) apiary. Journal of Invertebrate Pathology 91: 6973.CrossRefGoogle ScholarPubMed
Chen, SL (2001) Apiculture Science in China. Beijing, China: Agricultural Publishing House of China, pp.123131 (in Chinese).Google Scholar
Forêt, S and Maleszka, R (2006) Function and evolution of a gene family encoding odorant binding-like proteins in a social insect, the honey bee (Apis mellifera). Genome Research 16: 14041413.CrossRefGoogle Scholar
Forêt, S, Wanner, KW and Maleszka, R (2007) Chemosensory proteins in the honey bee: Insights from the annotated genome, comparative analyses and expressional profiling. Insect Biochemistry and Molecular Biology 37: 1928.CrossRefGoogle ScholarPubMed
Kucharski, R and Maleszka, R (2005) Microarray and real-time PCR analyses of gene expression in the honeybee brain following caffeine treatment. Journal of Molecular Neuroscience 27: 269276.CrossRefGoogle ScholarPubMed
Kukielka, D, Esperon, F, Higes, M and Sanchez-Vizcaino, JM (2008) A sensitive one-step real-time RT-PCR method for detection of deformed wing virus and black queen cell virus in honeybee Apis mellifera. Journal of Virological Methods 147: 275281.CrossRefGoogle ScholarPubMed
Li, HL, Lou, BG, Cheng, JA and Gao, QK (2007) The chemosensory protein of the Chinese honeybee, Apis cerana cerana: molecular cloning of cDNA, immunocytochemical localization and expression. Chinese Science Bulletin 52: 13551364.CrossRefGoogle Scholar
Li, HL, Zhang, YL, Gao, QK, Cheng, JA and Lou, BG (2008) Molecular Identification of cDNA, immunolocalization, and expression of a putative odorant-binding protein from an Asian honeybee, Apis cerana cerana. Journal of Chemical Ecology 34: 15931601.Google Scholar
Livak, KJ and Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402408.CrossRefGoogle ScholarPubMed
Michelette, ERF and Soares, AEE (1993) Characterization of preimaginal developmental stages in Africanized honey bee workers (Apis mellifera L.). Apidologie 24: 431440.CrossRefGoogle Scholar
Pelosi, P (1996) Perireceptor events in olfaction. Journal of Neurobiology 30: 319.3.0.CO;2-A>CrossRefGoogle ScholarPubMed
Pelosi, P, Zhou, JJ, Ban, LP and Calvello, M (2006) Soluble proteins in insect chemical communication. Cellular and Molecular Life Sciences 63: 16581676.CrossRefGoogle ScholarPubMed
Rachinsky, A, Strambi, C, Strambi, A and Hartfelder, K (1990) Caste and metamorphosis: hemolymph titers of juvenile hormone and ecdysteroids in last instar honeybee larvae. General and Comparative Endocrinology 79: 3138.CrossRefGoogle ScholarPubMed
Rheault, MR, Okech, BA, Keen, SB et al. (2007) Molecular cloning, phylogeny and localization of AgNHA1: the first Na+/H+ antiporter (NHA) from a metazoan, Anopheles gambiae. Journal Experimental Biology 210: 38483861.CrossRefGoogle ScholarPubMed
Yang, CH (1998) The development and lives of male and worker bees of Apis cerana cerana. Journal of Honey Bee 8: 16 (in Chinese).Google Scholar