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Establishment of a highly efficient regeneration system for in vitro culture of young wheat spikes

Published online by Cambridge University Press:  12 February 2007

Zhao Lin-Shu
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Liu Lu-Xiang
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wang Jing
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zheng Qi-Cheng
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Guo Hui-Jun
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhao Shi-Rong
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Chen Wen-Hua
Affiliation:
Key Laboratory of Agricultural Nuclear Technology and Space Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Corresponding
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Abstract

This study used three winter wheat (Triticum aestivum L.) genotypes (H6756, H311 and SP8581) to compare the effects of sampling time, callus induction media, differentiation media and rooting media on in vitro culture of young spikes in wheat. In all these three genotypes, the frequencies of green plantlet differentiation were high when their young spikes were cultured between the stages of protective glume primordium formation and pistil and stamen primordium formation, but low at other stages. The optimum medium for callus induction was Murashige and Skoog (MS) medium+2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The optimum green plantlet differentiation medium was MS medium. Some abnormal plantlets regenerated from calli. When these plantlets were transferred to another differentiation medium [MS+1.0 mg/l 1-naphthaleneacetic acid (NAA)+0.2 mg/l 6-benzylaminopurine (6-BA)], shoot formation and elongation were induced. This allowed 90.91% of them to develop into normal green plantlets. The optimum rooting medium was 1/2MS+0.2 mg/l 3-Indolylacetonitrile (IAA)+80 g/l sucrose. An efficient regeneration system for young spike culture of wheat was set up based on such methods. Using this wheat-regeneration system, young spikes and immature embryos of 17 genotypes of wheat were in vitro cultured to study and compare the callus induction frequencies and green plantlet differentiation frequencies. The results of two successive years showed that in 15 out of the 17 genotypes (88.24%) the green plantlet differentiation frequencies were higher than those of immature embryos by 6.2–65.1%. These results showed that the regeneration system established in this trial for young spike culture of wheat was effective.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

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