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Differences of cytosine methylation in parental lines and F1 hybrids of Large White×Meishan crosses and their effects on F1 performance

Published online by Cambridge University Press:  12 February 2007

Jiang Cao-De
Key Laboratory of Grazers and Herbivores of Chongqing, Southwest Agricultural University, Chongqing 400716, China
Deng Chang-Yan*
Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China
Xiong Yuan-Zhu
Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China
*Corresponding author: Email:


In order to probe the effect of methylation on heterosis, the methylation-sensitive arbitrarily primed polymerase chain reaction (AP-PCR) technique was adopted to amplify pig genome DNA with 40 single arbitrary primers. The material involved parental lines and F1 hybrids of Large White×Meishan crosses. Nineteen differentially methylated sites with RsaI+HpaII digestion and 14 differentially methylated sites with RsaI+MspII digestion between parental lines and the hybrid were found. All fragments detected in this study were grouped into four classes: (1) the same level of methylation in both parental lines and the hybrid; (2) the same level of methylation in one parent and the hybrid; (3) an increased level of methylation in the hybrid compared to the parents, and (4) a decreased level of methylation in the hybrid. Five sites had significant effects on seven traits (P<0.05). Sequence analysis showed that three sequences had a high-identity match in GenBank (greater than 87%) and two sequences had no match in the database. The percentage of G+C in three sequences was over 50, and the observed/expected CpG of all sequences was above 0.6. Furthermore, one sequence contained G/C boxes. This study demonstrated that the sites in CpG islands within a gene promoter were differentially methylated in the hybrid compared to parental lines; methylated sites contributed differentially to F1 performance, showing that heterosis could benefit from either expression or repression of some genes.

Research Article
Copyright © China Agricultural University and Cambridge University Press 2005

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Arthur, PF, Renand, G and Krauss, D (2001) Genetic and phenotypic relationships among different measures of growth and feed efficiency in young Charolais bulls. Livestock Production Science 58: 131139.CrossRefGoogle Scholar
Bird, AP (1986) CpG-rich islands and the function of DNA methylation. Nature 321: 209212.CrossRefGoogle ScholarPubMed
Cheng, NH, Yang, JS, Gao, YP et al. , (1996) Differential display of mRNA between hybrid F 1 and its parental inbred inbreed lines. Chinese Science Bolletin 41: 451454.Google Scholar
Cheng, NH, Yang, JS, Gao, YP et al. , (1997) Alteration of gene expression in rice hybrid F 1 and its parental seedlings. Acta Biotanica Sinica 39(4)379382 (in Chinese with English abstract).Google Scholar
Doelfler, W (1983) DNA methylation and gene activity. Annual Review of Biochemistry 52: 93124.CrossRefGoogle Scholar
Finnegan, EJ, Brettell, RIS and Dennis, ES (1993) The role of DNA methylation in the regulation of plant gene expression. In: Jost, JP and Saluz, HP (editors) DNA Methylation: Molecular Biology and Biological Significance. Basel: Birkhauser, pp. 218261.CrossRefGoogle Scholar
Fitzhugh, JHA and Taylor, SCS (1971) Genetic analysis of degree of maturity. Journal of Animal Science 33(4): 17725.CrossRefGoogle ScholarPubMed
Gardiner-Garden, M and Frommer, M (1987) CpG islands in vertebrate genomes. Journal of Molecular Biology 196: 261282.CrossRefGoogle ScholarPubMed
Gonzalgo, ML, Liang, G, Spruck III, CH (1997) Identification and characterization of differentially methylated regions of genomic DNA by methylation-sensitive arbitratily primed PCR. Cancer Research 57(4): 594599.Google Scholar
Gruenbaum, Y, Naveh-Many, Y, Cedar, H et al. , (1981) Sequence specificity of methylation in higher plant DNA. Nature 292: 860862.CrossRefGoogle ScholarPubMed
Jiang, XP, Xiong, YZ, Liu, GQ et al. , (2003) Effects of individual gene heterosity on growth traits in swine. Acta Genetica Sinica 30(5): 431436 (in Chinese with English abstract).Google Scholar
Liang, GN, Carol, E, Salem, MC et al. , (1998) DNA methylation differences associated with tumor tissues identified by genome scanning analysis. Genomics 53(3): 260268.CrossRefGoogle ScholarPubMed
Liang, GN, Mard, L and Gonzalgo, CS (2002) Identification of DNA methylation differences during tumorigenesis by methylation-sensitive arbitrarily primed polymerase chain reaction. Methods 27: 150155.CrossRefGoogle ScholarPubMed
McClelland, M, Nelson, M and Raschke, E (1994) Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucleic Acids Research 22(17): 36403659.CrossRefGoogle ScholarPubMed
Okano, M, Bell, DW, Haber, D et al. , (1999) DNA methyltranferase Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99: 247257.CrossRefGoogle Scholar
Reik, W and Suranic, A (1997) Genomic Imprinting. Oxford, UK: IRL Press.CrossRefGoogle ScholarPubMed
Sambrook, J and Russell, DW (1989) Molecular Cloning: a Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.Google Scholar
Xiong, YZ (1999) Introduction to Breeding Pig Measurement. Bejing: China Agricultural Press(in Chinese).Google Scholar
Xiong, LZ, Yang, GP, Xu, CG et al. , (1998) Relationships of differential gene expression in leaves with heterosis and heterozygosity in a rice diallel cross. Molecular Breeding 4: 129136.CrossRefGoogle Scholar
Zhang, Q, Zhou, ZQ, Yang, GP et al. , (1996) Molecular marker heterozygosity and hybrid performance in indica and Japonica rice. Theory and Application Genetics 93: 12181224.CrossRefGoogle ScholarPubMed