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TREHALASE ACTIVITY IN THE SILK GLANDS OF THE SILKWORM, BOMBYX MORI (LEPIDOPTERA: BOMBYCIDAE)

  • Shuya Shimada (a1)

Abstract

(1) Crude extracts prepared from the silk glands of the silkworm, Bombyx mori L. contain trehalase activity. (2) Trehalase in the silk glands has a pH of 5.5 and a Km of 0.71 mM. The activity of the enzyme is inhibited by divalent cations such as Mn, Cu, and Zn. (3) By histochemical methods, it is shown that trehalase is localized in the periphery of the silk gland cells, especially in the tunica propria and tunica intima. (4) Trehalase activity is low in fifth instar and increases greatly in spinning stages, after which the activity decreases.

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Dahlman, D. L. 1971. Purification and properties of trehalase from tobacco hornworm larvae. J. Insect Physiol. 17: 16771687.
Duchateau-Bosson, G. et al. , 1963. Contributions à la biochimia du ver a soie. XXVII. Trehalose, trehalase et mue. Archs int. Physiol. Biochim. 71: 566576.
Horie, Y. 1959. Physiological studies on the alimentary canal of the silkworm, Bombyx mori. II. Carbohydrases in the digestive fluid and in the midgut tissue. Bull. seric. Exp. Stn Japan 15: 365382.
Jensen, D. E. and Buckner, B. A.. 1973. Soluble and particle-bound trehalase in extracts from larvae, pupae, and adults of Musca domestica. J. Insect Physiol. 19: 22552259.
Lowry, O. H. et al. , 1951. Protein measurement with the folin phenol reagent. J. biol. Chem. 193: 265275.
Marzluf, G. A. 1969. Studies of trehalase and sucrase of Drosophila melanogaster. Arch. Biochem. Biophys. 134: 818.
Saito, S. 1960. Trehalase of the silkworm, Bombyx mori. Purification and properties of the enzyme. J. Biochem., Tokyo 48: 101109.
Somogyi, M. 1952. Notes on sugar determination. J. biol. Chem. 195: 1923.
Wyatt, G. R. 1967. The biochemistry of sugars and polysaccharides in insects, pp. 287360. In Beament, J. W. L. et al. (Eds.), Advances in insect physiology, Vol. 4. Academic Press, London.
Yamashita, O. 1965. Carbohydrate metabolism during embryonic development of the silkworm, Bombyx mori. J. seric. Sci., Tokyo 34: 17.
Yamashita, O. 1969. Studies on the glycogen metabolism in pupal ovaries of the silkworm, Bombyx mori L. J. seric. Sci., Tokyo 38: 329339.

TREHALASE ACTIVITY IN THE SILK GLANDS OF THE SILKWORM, BOMBYX MORI (LEPIDOPTERA: BOMBYCIDAE)

  • Shuya Shimada (a1)

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