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  • T. S. Sahota (a1), F. G. Peet (a1) and P. H. Bartels (a2)


Samples of the Douglas-fir bark beetle, Dendroctonus pseudotsugae Hopk., taken from two different segments of the infested bole were different in gallery production, egg deposition, and yolk deposition. The same two groups were distinguished by comparing fat body cell nuclei taken before the onset of the reproductive period. Techniques for comparing nuclei involved creation and analysis of digital images of nuclei. These groups were indistinguishable from each other, at that stage, by any other known method. The two groups differed in total absorbance of Fuelgen-stained nuclei (DNA content) and nuclear area. Significance of these findings is discussed in relation to early detection of reproduction-related quality differences and population dynamics of bark beetles.

Les dendroctones du Douglas, Dendroctonus pseudotsugae Hopk., prélevés dans deux sections différentes du tronc se sont révélés différents quant à la production de galeries, au dépôt d'oeufs et au dépôt de vitellus. Une distinction a été établie entre les deux groupes par comparaison des noyaux cellulaires des corps gras prélevés avant le déclenchement de la période de la reproduction. Les deux groupes étaient indistinguables à cette période par toutes les autres méthodes connues. Les techniques de comparaison des noyaux comportent la création et l'analyse d'images numériques. Les deux groupes se sont trouvés différents quant à la grandeur et à l'absorption complète des noyaux cellulaires (contenu ADN) teints avec la teinture Fuelgen. L'importance de ces résultats est examinée du point de vue de la détection précoce des différences qualitatives des dendroctones quant à la reproduction.



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Atkins, M. D. 1967. The effect of rearing temperatures on the size and fat content of the Douglas-fir beetle. Can. Ent. 99: 181187.
Bartels, P. H. 1981. Numerical evaluation of cytologic data, VII, multivariate significance tests. Anal. Quant. Cytol. 3: 18.
Bartels, P. H. and Olson, G. B.. 1980. Computer analysis of lymphocyte images. pp. 1–99 in Catsimpoolas, N. (Ed.), Methods of Cell Separation. Plenum Press, New York.
Berryman, A. A. 1974. Dynamics of bark beetle populations toward a general productivity model. Environ. Ent. 3: 579585.
Berryman, A. A. and Ashraf, M.. 1970. Effects of Abies grandis resin on the attack behaviour and brood survival of Scolytus ventralis (Coleoptera: Scolytidae). Can. Ent. 102: 12291236.
Box, G. E. P. 1949. A general distribution theory for a class of likelihood criteria. Biometrika 36: 317346.
Cole, W. E. 1973. Crowding effects among single-age larvae of the mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Scolytidae). Environ. Ent. 2: 285293.
Cook, L. M. 1961. Influence of larval environment on adult size and fecundity in the moth Panazia dominula L. Nature, Lond. 192: 282.
Coulson, R. N., Mayyasi, A. M., Foltz, J. L., Hain, F. P., and Martin, W. C.. 1976. Resource utilization by the southern pine beetle, Dendroctonus frontalis (Coleoptera: Scolytidae). Can. Ent. 108: 353362.
Drooz, A. T. 1965. Some relationships between host, egg potential and pupal weight of the elm spanworm, Ennomos subsignarius (Lepidoptera: Geometridae). Ann. ent. Soc. Am. 58: 243245.
Farris, S. H., Sahota, T. S., Ibaraki, A., and Thomson, A. J.. 1982. Use of pectinase to dissociate plant nuclei for squash preparation. Effect of hydration procedures. Stain Technol. 57: 283288.
Hotelling, H. 1931. The generalization of Student's ratio. Ann. Math. Statist. 2: 360378.
McGhehey, J. H. 1971. Female size and egg production of the mountain pine beetle, Dendroctonus ponderosae Hopkins. Northern Forest Research Centre, Edmonton, Alberta. Information Rep. NOR-X-9. 18 pp.
McMullen, L. H. and Atkins, M. D.. 1961. Intraspecific competition as a factor in the natural control of the Douglas-fir beetle. Forest Sci. 7: 197203.
Millar, C. A. 1957. A technique for estimating the fecundity of natural populations of spruce budworm. Can. J. Zool. 35: 113.
Morrison, D. F. 1967. Multivariate Statistical Methods. McGraw Hill, New York. 120 pp.
Peet, F. G. and Sahota, T. S.. A computer assisted cell identification system. Anal. Quantit. Cytol. in press.
Reid, R. W. 1962. Biology of the mountain pine beetle, Dendroctonus ponderosae Hopkins, in the East Kootenay region of British Columbia. II. Behaviour in the host, fecundity and internal changes in the female. Can. Ent. 94: 605613.
Reid, R. W. 1963. Biology of the mountain pine beetle, Dendroctonus ponderosae Hopkins, in the East Kootenay region of British Columbia. III. Interaction between the beetle and its host, with emphasis on brood mortality and survival. Can. Ent. 95: 225238.
Sahota, T. S. and Ibaraki, A.. 1979. Effect of host tree activity on the rate of yolk protein deposition in Dendroctonus rufipennis (Coleoptera: Scolytidae). Can. Ent. 111: 13191323.
Sahota, T. S., Ibaraki, A., Heywood, F. G., Farris, S. H., and Van der Wereld, A. M.. 1981. Image enhancement for light microscopy. Stain Technol. 56: 361366.
Sahota, T. S. and Thomson, A. J.. 1979. Temperature induced changes in the rates of reproductive processes in Dendroctonus rufipennis (Coleoptera: Scolytidae). A new approach to detecting changes in population quality. Can. Ent. 111: 10691078.
Thomson, A. J. and Sahota, T. S.. 1981. Competition and population quality in Dendroctonus rufipennis (Coleoptera: Scolytidae). Can. Ent. 113: 177183.
Wagner, T. L., Fargo, W. S., Keeley, L. L., Coulson, R. N., and Cover, J. D.. 1982. Effects of sequential attack on gallery construction, oviposition and re-emergence by Dendroctonus frontalis (Coleoptera: Scolytidae). Can. Ent. 114: 491502.
Wellington, W. G. 1976. Applying behavioural studies in entomological problems. pp. 8797 in Anderson, J. F. and Kaya, H. K. (Eds.), Perspectus in Forest Entomology. Academic Press, New York.
Wellington, W. G. 1977. Returning the insect to insect ecology: Some consequences for pest management. Environ. Ent. 6: 18.
Wellington, W. G. and Maelzer, D. A.. 1967. Effects of farnesyl methyl ether on the reproduction of western tent caterpillar, Malacosoma pluviale: Some physiological, ecological, and practical implications. Can. Ent. 99: 249263.
Wilks, S. S. 1932. Certain generalizations in the analysis of variance. Biometrika 24: 471494.


  • T. S. Sahota (a1), F. G. Peet (a1) and P. H. Bartels (a2)


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