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Phylogenetic placement and evidence for horizontal transfer of Wolbachia in Plutella xylostella (Lepidoptera: Plutellidae) and its parasitoid, Diadegma insulare (Hymenoptera: Ichneumonidae)

Published online by Cambridge University Press:  02 April 2012

Philip D. Batista ,
B. Andrew Keddie ,
Lloyd M. Dosdall  and
Harriet L. Harris
Philip D. Batista*
Affiliation:
Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6E 2E9
B. Andrew Keddie
Affiliation:
Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6E 2E9
Lloyd M. Dosdall
Affiliation:
Department of Agricultural, Food and Nutritional Science, 4–10 Agriculture/Forestry Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2P5
Harriet L. Harris
Affiliation:
Department of Biological Sciences, University of Alberta, Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6E 2E9, and Department of Biology and Environmental Sciences, Concordia University College of Alberta, Edmonton, Alberta, Canada T5B 4E4
*
1 Corresponding author (e-mail: pbatista@ualberta.ca).
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Abstract

The diamondback moth, Plutella xylostella (L.), is a global pest of cruciferous crops (Brassicaceae). It has developed resistance to virtually all known insecticides, and biological control has become an important management tool. In North America the parasitoid Diadegma insulare (Cresson) has been used successfully to reduce diamondback moth populations. We document the presence of the α-proteobacterial endosymbiont Wolbachia and its associated bacteriophage WO in P. xylostella and D. insulare and examine the phylogenetic relationships of Wolbachia and WO in both host species. Our results suggest that Wolbachia and WO have been horizontally transferred in this insect–parasitoid system in recent evolutionary history. Knowledge of the dynamics of Wolbachia in P. xylostella and D. insulare may be an important factor in future control of this pest in the field.

Résumé

La fausse teigne des crucifères, Plutella xylostella (L.), est un ravageur des récoltes de crucifères (Brassicaceae) à l'échelle de la planète. Elle a développé une résistance à presque tous les insecticides connus et la lutte biologique est un outil important pour sa gestion. En Amérique du Nord, le parasitoïde Diadegma insulare (Cresson) a servi à réduire avec succès les populations de la fausse teigne des crucifères. Nous démontrons la présence d'un Wolbachia endosymbionte α-protéobactérien et de son bactériophage WO chez P. xylostella et D. insulare et nous examinons les relations phylogénétiques de Wolbachia et de WO chez les deux espèces d'hôtes. Nos résultats indiquent que Wolbachia et WO ont subi un transfert horizontal dans ce système insecte-parasitoïde durant l'histoire évolutive récente. La connaissance de la dynamique de Wolbachia chez P. xylostella et D. insulare pourrait être un facteur important dans le contrôle de ce ravageur en nature dans le futur.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 142 , Issue 1 , February 2010 , pp. 57 - 64
Copyright
Copyright © Entomological Society of Canada 2010

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