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Nucleopolyhedrovirus infection in obliquebanded leafroller (Lepidoptera: Tortricidae)

  • I. Pronier (a1), J. Paré (a1), J-C Wissocq (a1) and C. Vincent (a2)

Abstract

A virus isolated from obliquebanded leafroller, Choristoneura rosaceana (Harris), larvae collected in an apple, Malus domestica Borkh. (Rosaceae), orchard of Saint-Joseph-du-Lac (Quebec, Canada) was studied. Microscopic studies revealed that it was a uninucleocapsid nucleopolyhedrovirus from the family Baculoviridae. Larval mortality was approximately 75% (0% mortality in control group) in larvae infected as third instars immersed in a suspension of 1.7 × 108 occlusion bodies/mL. The average time for larval mortality was 23 ± 3 d after treatment. The majority (95.5%) of infected larvae died as fifth or sixth instars. Infection was observed primarily in fat body cells, and occasionally in the tracheal matrix and epidermis. Mean larval development time of infected larvae surviving to pupae was 20 ± 3 d, significantly greater than the 18 ± 3 d observed in control larvae. Adult emergence was significantly lower in pupae of treated larvae (73.6%) than in the control group (93.5%). Our work constitutes the first baseline study of naturally occurring virus of the obliquebanded leafroller.

Un virus a été isolé de chenilles de la tordeuse à bandes obliques, Choristoneura rosaceana (Harris), collectées dans un verger de pommiers, Malus domestica Borkh. (Rosaceae), à Saint-Joseph-du-Lac (Québec, Canada). Des observations microscopiques ont révélé qu’il s’agissait d’un virus de la polyhédrose nucléaire, possédant une nucléocapside par enveloppe, de la famille des Baculoviridae. La mortalité larvaire était d’environ 75% (aucune mortalité dans le groupe témoin) lorsque les chenilles étaient infectées au troisième stade par immersion dans une suspension de 1,7 × 108 corps d’inclusions polyédriques/mL. La durée de vie moyenne avant la mort de ces individus était de 23 ± 3 j. La majorité (95,5%) des chenilles malades étaient mortes au cours du cinquième ou sixième stade larvaire. L’infection virale était localisée principalement dans le tissu adipeux et occasionnellement dans la matrice trachéale et le tégument. Pour les individus ayant survécu à l’infection, la durée moyenne du développement larvaire des individus infectés ayant atteint le stade de chrysalide de 20 ± 3 j était significativement allongée par rapport aux 18 ± 3 j des individus témoins. L’émergence des adultes était réduite à 73,6% pour les individus traités par le virus en comparaison des 93,5% observés dans le groupe témoin. Notre travail constitue la première étude de base concernant un virus qui survient de façon naturelle chez la tordeuse à bandes obliques.

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Corresponding author

1 Corresponding author (e-mail: vincentch@em.agr.ca).

References

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Nucleopolyhedrovirus infection in obliquebanded leafroller (Lepidoptera: Tortricidae)

  • I. Pronier (a1), J. Paré (a1), J-C Wissocq (a1) and C. Vincent (a2)

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