Skip to main content Accessibility help

Molecular evidence for sympatric taxa within Pemphigus betae (Hemiptera: Aphididae: Eriosomatinae)

  • R. G. Foottit (a1), K. Floate (a2) and E. Maw (a1)


Mitochondrial DNA cytochrome c oxidase subunit 1 (DNA barcode) and nuclear microsatellite flanking region sequences were used to analyse populations of putative “sugarbeet root aphid”, Pemphigus betae Doane, from sites in Alberta, Canada. Three sympatric genotypes were revealed, identified as P. betae, P. populivenae Fitch, and an undetermined third species. All three genotypes formed morphologically indistinguishable galls on the same set of cottonwood (Populus L., Salicaceae) host species, often on the same tree. Gall morphology is frequently used to identify Pemphigus species. Our results indicate that this practice may be unreliable for these three taxa at least.

Les séquences de la sous-unité 1 de la cytochrome c oxydase de l'ADN mitochondrial (code à barres de l'ADN) et de la région flanquante des microsatellites nucléaires nous ont servi à analyser les populations de l'espèce putative du « puceron de la betterave à sucre », Pemphigus betae Doane, de sites de l'Alberta (Canada). Il existe trois génotypes sympatriques, identifiés comme P. betae, P. populivenae Fitch et une troisième espèce non identifiée. Les trois génotypes forment tous des galles qui sont impossibles à distinguer sur le même ensemble d'espèces-hôtes de peupliers (Populus L., Salicaceae), souvent sur le même arbre. La morphologie des galles sert souvent à identifier les espèces de Pemphigus; nos résultats indiquent que cette procédure n’est pas fiable au moins pour ces trois taxons.

[Traduction par la Rédaction]


Corresponding author

1 Corresponding author (e-mail:


Hide All
Blackman, R.L., and Eastop, V.F. 2000. Aphids on the world's crops: an identification and information guide. 2nd ed. John Wiley & Sons, Chichester, UK.
Cranshaw, W., Kondratieff, B.C., and Qian, T. 1990. Insects associated with quinoa, Chenopodium quinoa, in Colorado. Journal of the Kansas Entomological Society, 63: 195199.
deWaard, J.R., Ivanova, N.V., Hajibabaei, M., and Hebert, P.D.N. 2008. Assembling DNA barcodes: analytical protocols. In Environmental genomics: methods in molecular biology. Vol. 410. Edited by Martin, C.C.. Humana Press, Totowa, New Jersey. pp. 275293.
Floate, K.D. 2004. Extent and patterns of hybridization among the three species of Populus that constitute the riparian forest of southern Alberta, Canada. Canadian Journal of Botany, 82: 253264. doi:10.1139/b03-135.
Floate, K.D., and Whitham, T.G. 1993. The “hybrid bridge” hypothesis: host shifting via plant hybrid swarms. The American Naturalist 141: 651662. PMID:19426003 doi:10.1086/285497.
Floate, K.D., Martinsen, G.D., and Whitham, T.G. 1997. Cottonwood hybrid zones as centers of abundance for gall aphids in western North America: importance of relative habitat size. Journal of Animal Ecology, 66: 179188. doi:10.2307/6020.
Foottit, R.G., Maw, H.E.L., von Dohlen, C.D, and Hebert, P.D.N. 2008. Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes. Molecular Ecology Resources, 8: 11891201. doi:10.1111/j.1755-0998.2008.02297.x.
Foottit, R.G., Maw, H.E.L., and Pike, K.S. 2009. DNA barcodes to explore diversity in aphids (Hemiptera Aphididae and Adelgidae). Redia, 92: 8791.
Grigarick, A.A., and Lange, W.H. 1962. Host relationships of the sugar-beet root aphid in California. Journal of Economic Entomology, 55: 760764.
Harper, A.M. 1959. Gall aphids on poplar in Alberta I. Descriptions of galls and distributions of aphids. The Canadian Entomologist, 91: 489496. doi: 10.4039/Ent91489-8.
Harper, A.M. 1963. Sugar-beet root aphid, Pemphigus betae Doane (Homoptera: Aphididae), in southern Alberta. The Canadian Entomologist, 95: 863873. doi:10.4039/Ent95863-8.
Hebert, P.D.N., Cywinska, A., Ball, S.L., and deWaard, J.R. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270: 313322.
Hutchison, W.D., and Campbell, C.D. 1994. Economic impact of sugarbeet root aphid (Homoptera: Aphididae) on sugarbeet yield and quality in southern Minnesota. Journal of Economic Entomology, 87: 465475.
Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 11120. PMID:7441778 doi:10.1007/BF01731581.
Maxson, A.C. 1916. Some unpublished notes on Pemphigus betae Doane. Journal of Economic Entomology, 9: 500504.
Maxson, A.C., and Knowlton, G.F. 1929. The tribe Pemphigini (Aphididae) in Utah. Annals of the Entomological Society of America, 32: 251271.
Moran, N.A., and Whitham, T.G. 1988. Evolutionary reduction of complex life cycles: loss of host-alternation in Pemphigus (Homoptera: Aphididae). Evolution, 42: 717728. doi:10.2307/2408863.
Palmer, M.A. 1952. Aphids of the Rocky Mountain region. Vol. 5. Thomas Say Foundation.
Posada, D, and Crandall, K.A. 1998. Modeltest: testing the model of DNA substitution. Bioinformatics, 14: 817818. PMID:9918953 doi:10.1093/bioinformatics/14.9.817.
Ratnasingham, S., and Hebert, P.D.N. 2007. BOLD: the Barcode of Life Data System ( Molecular Ecology Notes, 7: 355364. PMID:18784790 doi:10.1111/j.1471-8286.2007.01678.x.
Remaudière, G., and Remaudière, M. 1997. Catalogue des Apididae du monde: Homoptera Aphidoidea. [Catalogue of the world's Aphididae: Homoptera Aphidoidea]. INRA Editions, Versailles, France.
Saitou, N., and Nei, M. 1987. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406425. PMID:3447015.
Smith, C.F. 1984. Pemphiginae in North America. In Proceedings of the International Aphidological Symposium, Jablonna, Poland, 5211 April 1981. Edited by Szelegiewicz, H.. Polska Akademia Nauk, Instytut Zoologii, Ossolineum, Wroclaw, Poland. pp. 277302.
Stroyan, H.L.G. 1970. Three new aphid species from North America. Proceedings of the Royal Entomological Society of London Series B: Taxonomy, 39: 153162.
Summers, C.G., and Newton, A.S. 1989. Economic significances of Pemphigus populivenae Fitch (Homoptera: Aphididae) in California. Journal of Applied Agricultural Research, 4: 162167.
Swofford, D.L. 2003. PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts.
Whitham, T.G. 1989. Plant hybrid zones as sinks for pests. Science (Washington, D.C.), 244: 14901493.
Whitham, T.G., Martinsen, G.D., Floate, K.D., Dungey, H.S., Potts, B.M., and Keim, P. 1999. Plant hybrid zones affect biodiversity: tools for a genetic-based understanding of community structure. Ecology, 80: 416428. doi:10.1890/0012-9658(1999)080[0416:PHZABT]2.0.CO;2.

Molecular evidence for sympatric taxa within Pemphigus betae (Hemiptera: Aphididae: Eriosomatinae)

  • R. G. Foottit (a1), K. Floate (a2) and E. Maw (a1)


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed