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Effect of ultrasound on acetylcholinesterase activity in Helicoverpa armigera (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  02 April 2012

Yu-Ping Zha ,
Fen Xu ,
Qi-Cai Chen  and
Chao-Liang Lei
Yu-Ping Zha
Affiliation:
College of Plant Science and Technology and Key Laboratory of Insect Resource Utilization and Sustainable Pest Management of Hubei Province, Huazhong Agricultural University, Wuhan 430070, People's Republic of China, and College of Life Sciences, Huazhong Normal University, Wuhan 430079, People's Republic of China
Fen Xu
Affiliation:
College of Physical Science and Technology, Huazhong Normal University, Wuhan 430079, People's Republic of China
Qi-Cai Chen
Affiliation:
College of Life Sciences, Huazhong Normal University, Wuhan 430079, People's Republic of China
Chao-Liang Lei*
Affiliation:
College of Plant Science and Technology and Key laboratory of Insect Resource Utilization and Sustainable Pest Management of Hubei Province, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
*
2Corresponding author (e-mail: ioir@mail.hzau.edu.cn).
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Abstract

It is well known that noctuid moths respond to ultrasound frequencies produced by insectivorous bats performing a series of evasive maneuvers such as loops, dives, rolls, and turns. Certain ultrasound frequencies may be considered an environmental stress factor for these moths, causing physiological and behavioral effects. We investigated changes in acetylcholinesterase activity of Helicoverpa armigera (Hübner) exposed to ultrasound produced from a commercial device (LHC20). Our results indicated that stress effects on acetylcholinesterase activity resulting from exposure to ultrasound do not differ according to sex, but effects on different developmental stages of H. armigera differ significantly depending on duration of exposure. Enzyme activity increased in adults after 20 min exposure to ultrasound and decreased in pupae after 30 and 50 min exposure. Enzyme activity in larvae was reduced after 20 min and increased after 40 and 60 min. The results of this study also indicate that stress caused by exposure to ultrasound could modulate the cholinergic system in H. armigera.

Résumé

Il est bien connu que les papillons de nuit noctuidés réagissent aux fréquences ultrasoniques produites par les chauves-souris insectivores en exécutant une série de manoeuvres d’évitement, telles que des boucles, des plongeons, des tonneaux et des virages. Certaines fréquences ultrasoniques peuvent être considérées comme des facteurs environnementaux de stress pour ces papillons de nuit, ce qui peut causer des effets physiologiques et comportementaux. Nous étudions les changements de l’activité de l’acétylcholinestérase chez Helicoverpa armigera (Hübner) lors d’expositions à des ultrasons produits par un appareil commercial (LCH20). Nos résultats indiquent que les effets du stress dû à l’exposition aux ultrasons sur l’activité de l’acétylcholinestérase sont les mêmes chez les deux sexes; il y a, cependant, des effets significativement différents chez les divers stades de développement d’H. armigera en fonction de la durée de l’exposition. L’activité enzymatique s’accroît chez les adultes exposés à un stress ultrasonique pendant 20 minutes et décroît chez les nymphes après 30 ou 50 minutes. Chez les larves, l’activité enzymatique décroît après une exposition de 20 minutes et augmente après une exposition de 40 ou 60 minutes. Notre étude indique aussi que le stress causé par l’exposition aux ultrasons pourrait moduler le système cholinergique chez H. armigera.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 140 , Issue 5 , October 2008 , pp. 563 - 568
Copyright
Copyright © Entomological Society of Canada 2008

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