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Effect of cold storage of eggs on the response of cabbage looper (Lepidoptera: Noctuidae) larvae to a nucleopolyhedrovirus

  • Maynard L. Milks (a1)

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Nucleopolyhedroviruses (NPVs) are naturally occurring entomopathogens that show promise as bioinsecticides against pest caterpillars. Determining the potential of a NPV as an insecticide requires conducting many laboratory assays. These experiments typically require a large number of larvae and it may be necessary to collect eggs from laboratory or wild sources for several days before an adequate supply is obtained. Fortunately, in many species of Lepidoptera, it is possible to synchronize rearing by storing eggs at 4°C.

Cabbage loopers (Trichoplusia ni Hübner) are widespread, highly destructive agricultural pests. Preliminary trials suggest that the single (S) nucleocapsid nucleopolyhedrovirus of cabbage loopers (TnSNPV) might be effective in controlling cabbage loopers (Jaques 1977); however, many additional studies are required before it can be commercially developed as a bioinsecticide.

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Hughes, P.R., Wood, H.A. 1986. In vivo and in vitro bioassay methods for baculoviruses. pp 130in Granados, R. and Federici, B. (Eds), Biology of Baculoviruses. Boca Raton, Florida: CRC Press
Kishaba, A.N., Henneberry, T.J. 1966. Effects of cold storage on egg viability of the cabbage looper and some aspects of the biology of the progeny survivors. Journal of Economic Entomology 59: 1169–71
Jaques, R.P. 1967. The persistence of a nuclear polyhedrosis virus in the habitat of the host insect, Trichoplusia ni. I. Polyhedra deposited on foliage. The Canadian Entomologist 99: 785–94
Jaques, R.P. 1977. Field efficacy of viruses infectious to the cabbage looper and imported cabbageworm on late cabbage. Journal of Economic Entomology 65: 757–60
Milks, M.L. 1997 a. The comparative biology and susceptibility of Trichoplusia ni (Lepidoptera: Noctuidae) lines to a nuclear polyhedrosis virus. Environmental Entomology 26: 839–48
Milks, M.L. 1997 b. Ingestion time does not affect the susceptibility of cabbage loopers to a nuclear polyhedrosis virus. Journal of Invertebrate Pathology 70: 165–6
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Effect of cold storage of eggs on the response of cabbage looper (Lepidoptera: Noctuidae) larvae to a nucleopolyhedrovirus

  • Maynard L. Milks (a1)

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