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DNA fingerprinting of tabanids (Diptera: Tabanidae) and their respective egg masses using PCR – restriction fragment profiling

Published online by Cambridge University Press:  02 April 2012

M. Iranpour ,
A.M. Schurko ,
G.R. Klassen  and
T.D. Galloway
M. Iranpour*
Affiliation:
Department of Entomology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
A.M. Schurko
Affiliation:
Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
G.R. Klassen
Affiliation:
Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
T.D. Galloway
Affiliation:
Department of Entomology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
*
1Corresponding author (e-mail: iranpour@ms.umanitoba.ca).
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Abstract

Polymerase chain reaction and subsequent restriction fragment profiling analysis were used to associate collected tabanid egg masses with their respective species of adult horse flies and deer flies (Diptera: Tabanidae) in Manitoba, Canada. The ribosomal DNA (rDNA) intergenic spacer between the 28S and 18S ribosomal RNA genes was used successfully to differentiate 34 species of adult tabanids representing five genera: Atylotus (1 sp.), Chrysops (10 spp.), Haematopota (1 sp.), Hybomitra (17 spp.), and Tabanus (5 spp.). rDNA was a suitable molecular target for identifying tabanid species because of the high level of interspecific variation when comparing fragment profiles among different species, and the corresponding minimal intraspecific variation among individuals of the same species. Restriction fragment profiles from 56 field-collected tabanid egg masses were compared with those previously obtained from adults of known species. Egg masses of five species were identified: Hybomitra lasiophthalma (Macquart), Hybomitra nitidifrons nuda (McDunnough), Chrysops aestuans van der Wulp, Chrysops excitans Walker, and Chrysops mitis Osten Sacken. We also provide physical descriptions of these tabanid egg masses along with pictures.

Résumé

La réaction en chaîne de la polymérase et l'analyse subséquente des profils des fragments de restriction nous ont servi à associer des masses d'oeufs de tabanidés récoltées en nature aux espèces correspondantes de taons et de mouches à chevreuil (Diptera: Tabanidae) au Manitoba, Canada. L'analyse de l'espace intergénétique ADNr des gènes 28S et 18S codant pour l'ARNr nous ont permis de distinguer avec succès 34 espèces des taons adultes appartenant à cinq genres (Atylotus (1 spp.), Chrysops (10 spp.), Haematopota (1 sp.), Hybomitra (17 spp.), et Tabanus (5 spp.)). L'ADNr est une bonne cible moléculaire pour l'identification des especès de taons parce qu'il y a un degré considérable de variation interspécifique entre les profils des fragments des différentes espèces et parce que la variation intraspécifique entre les indivus de la même espèce est minimale. Les profils des fragments de restriction des masses des oeufs de 56 espèces récoltées en nature ont ensuite été comparés avec ceux de masses d'oeufs de femelles identifiées à l'espèce. Les masses d'oeufs de cinq espèces ont été identifiées comme appartenant à Hybomitra lasiophthalma (Macquart), Hybomitra nitidifrons nuda (McDunnough), Chrysops aestuans van der Wulp, Chrysops excitans Walker et Chrysops mitis Osten Sacken. On trouvera la description et des photographies de ces masses d'oeufs.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 136 , Issue 5 , October 2004 , pp. 605 - 619
Copyright
Copyright © Entomological Society of Canada 2004

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References

Anderson, J.F. 1985. The control of horse flies and deer flies (Diptera: Tabanidae). Myia 3: 547–98Google Scholar
Baldridge, G.D., Fallon, A.M. 1992. Primary structure of the ribosomal DNA intergenic spacer from the mosquito Aedes albopictus. DNA and Cell Biology 11: 51–9CrossRefGoogle ScholarPubMed
Belkhiri, A., Buchko, J., Klassen, G.R. 1992. The 5S ribosomal RNA gene in Pythium species: two different genomic locations. Molecular Biology and Evolution 9: 1089–102Google ScholarPubMed
Burger, J.F. 1977. The biosystematics of immature Arizona Tabanidae (Diptera). Transactions of the American Entomological Society (Philadelphia) 103: 145–258Google Scholar
Burger, J.F. 1995. Catalog of Tabanidae (Diptera) of North America north of Mexico. Contributions on Entomology International 1: 1–100Google Scholar
Coen, E.S., Dover, G.A. 1983. Unequal exchanges and the coevolution of X and Y rDNA arrays in Drosophila melanogaster. Cell 33: 849–55Google Scholar
Cross, N.C.P., Dover, G.A. 1987. Tsetse fly rDNA: an analysis of structure and sequence. Nucleic Acids Research 15: 15–30CrossRefGoogle ScholarPubMed
Dover, G. 1982. Molecular drive: a cohesive mode of species evolution. Nature (London) 299: 111–7Google Scholar
Foster, C.H., Renaud, G.D., Hays, K.L. 1973. Some effects of the environment on oviposition by Chrysops (Diptera: Tabanidae). Environmental Entomology 2: 1048–50Google Scholar
Goodwin, J.T. 1976. Immature stages of some eastern Nearctic Tabanidae (Diptera). VII. Haematopota Meigen and Whitneyomyia Bequaert plus other Tabanini. Florida Entomologist 59: 343–51Google Scholar
Hine, J.S. 1906. Preliminary report on the horse flies of Louisiana with a discussion of remedies and natural enemies. Louisiana State Crop Pest Commission. Circular 6: 1–43Google Scholar
Hoppe, K.L., Dillwith, J.W., Wright, R.E., Szumlas, D.E. 1990. Identification of horse flies (Diptera: Tabanidae) by analysis of cuticular hydrocarbons. Journal of Medical Entomology 27: 480–6CrossRefGoogle ScholarPubMed
Hudson, A., Teskey, H.J. 1976. Morphological and biochemical characteristics of two forms of Hybomitra typhus (Diptera: Tabanidae). The Canadian Entomologist 108: 737–40CrossRefGoogle Scholar
Iranpour, M. 2002. Egg parasitoids of tabanids in Manitoba: prevalence, taxonomy, behaviour, and use of polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) to establish host–parasite interrelationships. PhD thesis, University of Manitoba, Winnipeg, ManitobaGoogle Scholar
Iranpour, M., Galloway, T.D. 2002. Hybomitra, Tabanus, and Chrysops spp. (Diptera: Tabanidae). pp 84–86in Mason, P.G., Huber, J.T. (Eds), Biological programmes in Canada 1981–2000. Wallingford, United Kingdom: CABI PublishingGoogle Scholar
Israelewski, N., Schmidt, E.R. 1982. Spacer size heterogeneity in ribosomal DNA of Chironomus thummi is due to a 120 bp repeat homologous to a predominantly centromeric repeated sequence. Nucleic Acids Research 10: 7689–700CrossRefGoogle ScholarPubMed
Jacobson, N.R., Hansens, E.J., Vrijenhoek, R.C., Swofford, D.L., Berlocher, S.H. 1981. Electrophoretic detection of a sibling species of the salt marsh greenhead, Tabanus nigrovittatus. Annals of the Entomological Society of America 74: 602–5CrossRefGoogle Scholar
Krinsky, W.L. 1976. Animal disease agents transmitted by horse flies and deer flies (Diptera: Tabanidae). Journal of Medical Entomology 13: 225–75Google Scholar
Marchand, W. 1917. Notes on the early stages of Chrysops. Journal of the New York Entomological Society 25: 149–63Google Scholar
McAlpine, J.F. 1961. Variation, distribution, and evolution of the Tabanus (Hybomitra) frontalis complex of horse flies (Diptera: Tabanidae). The Canadian Entomologist 93: 894–924Google Scholar
McKeever, S., French, F.E. 1992. Observation on the laboratory life history of Chrysops atlanticus, C. univittatus and C. vittatus (Diptera: Tabanidae). Journal of Entomological Science 27: 458–60CrossRefGoogle Scholar
Pechuman, L.L. 1981. The horse flies and deer flies of New York (Diptera: Tabanidae). Search Agriculture 18: 1–66Google Scholar
Perich, M.J., Wright, R.E., Lusky, K.S. 1986. Impact of horse flies (Diptera: Tabanidae) on beef cattle. Journal of Economic Entomology 79: 128–31CrossRefGoogle ScholarPubMed
Pomonis, J.G. 1989. Cuticular hydrocarbons of the screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae): isolation, identification, and quantification as a function of age, sex, and irradiation. Journal of Chemical Ecology 15: 2301–18Google Scholar
Pomonis, J.G., Mackley, J.W. 1985. Gas chromatographic composition profiles of surface lipid extracts from screwworm compared by age, sex, colonization and geography. Southwestern Entomologist 10: 65–76Google Scholar
Ralley, W.E., Galloway, T.D., Crow, G.H. 1993. Individual and group behaviour of pastured cattle in response to attack by biting flies. Canadian Journal of Zoology 71: 725–34CrossRefGoogle Scholar
Roberts, R.H. 1966. A technique for rearing the immature stages of Tabanidae (Diptera). Entomological News 77: 79–82Google Scholar
Roberts, R.H., Dicke, R.J. 1964. The biology and taxonomy of some immature Nearctic Tabanidae (Diptera). Annals of the Entomological Society of America 57: 31–40Google Scholar
Schafer, M., Wyman, A.R., White, R. 1981. Length variation in the non-transcribed spacer of Calliphora erythrocephala ribosomal DNA is due to a 350 base-pair repeat. Journal of Molecular Biology 146: 179–99Google Scholar
Segal, B. 1936. Synopsis of the Tabanidae of New York, their biology and taxonomy. I. The genus Chrysops Meigen. Journal of the New York Entomological Society 44: 51–78Google Scholar
Sutton, B.D., Carlson, D.A. 1997. Cuticular hydrocarbon variation in the Tabanidae (Diptera): the Tabanus nigrovittatus complex of the North America Atlantic coast. Annals of the Entomological Society of America 90: 542–9Google Scholar
Tashiro, H., Schwardt, H.H. 1953. Some natural enemies of horseflies in New York. Journal of Economic Entomology 46: 680–1Google Scholar
Taylor, D.B., Szalanski, A.L., Peterson, R.D. 1996. Identification of screwworm species by polymerase chain reaction–restriction fragment length polymorphism. Medical and Veterinary Entomology 10: 63–70Google Scholar
Teskey, H.J. 1969. Larvae and pupae of some eastern North American Tabanidae (Diptera). Memoirs of the Entomological Society of Canada 63: 1–147Google Scholar
Teskey, H.J. 1990. The horse flies and deer flies of Canada and Alaska (Diptera: Tabanidae). Part 16. The Insects and Arachnids of Canada. Agriculture Canada Research Branch Report 1838Google Scholar
Thompson, P.H., Krauter, P.C. 1978. Rearing of Texas Tabanidae (Diptera). I. Collection, feeding, and maintenance of coastal marsh species. Proceedings of the Entomological Society of Washington 80: 616–25Google Scholar
Wellauer, P.K., Dawid, I.B. 1978. Ribosomal DNA in Drosophila melanogaster. II. Heteroduplex mapping of cloned and uncloned rDNA. Journal of Molecular Biology 126: 769–82Google Scholar
Wu, C.C.N., Fallon, A.M. 1998. Analysis of a ribosomal DNA intergenic spacer region from the yellow fever mosquito, Aedes aegypti. Insect Molecular Biology 7: 19–29Google Scholar