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Assessment of Metarhizium anisopliae (Clavicipitaceae) and its vector, Scleroderma guani (Hymenoptera: Bethylidae), for the control of Monochamus alternatus (Coleoptera: Cerambycidae)

Published online by Cambridge University Press:  22 December 2014

M. Xu ,
F.Y. Xu ,
Y.P. Liu ,
Y.S. Pan  and
X.Q. Wu
M. Xu
Affiliation:
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China Forestry Academy of Jiangsu Province, Nanjing 211153, Dongshanqiao, China
F.Y. Xu*
Affiliation:
Forestry Academy of Jiangsu Province, Nanjing 211153, Dongshanqiao, China
Y.P. Liu
Affiliation:
Forestry Academy of Jiangsu Province, Nanjing 211153, Dongshanqiao, China
Y.S. Pan
Affiliation:
Suqian Forestry Bureau, Suqian City 223800, China
X.Q. Wu
Affiliation:
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
*
1Corresponding author (e-mail: xufuyuan@aliyun.com).
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Abstract

The pinewood nematode, Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle (Aphelenchida: Parasitaphelenchidae), is a major disease of pines forests in China. Its vector, Monochamus alternatus Hope (Coleoptera: Cerambycidae), has been the focus of an intensive study to improve the efficiency of the parasitoid Scleroderma guani Xiao and Wu (Hymenoptera: Bethylidae) by using the parasitoid both as a larval parasitoid in its own right and additionally as a vector for the entomophagous pathogen Metarhizium anisopliae (Metchnikoff) Sorokin (Clavicipitaceae). Twenty-one M. anisopliae strains were screened and the best four tested for sporulation capabilities at a range of temperatures. The best strain was then evaluated at several sporulation concentrations to define the lethal concentration 50 (LC50) against the larvae of M. alternatus. Scleroderma guani was inoculated with the best strain and tested against larvae of M. alternatus, which were constrained singly in glass vials. Three S. guani females per vial infected with M. anisopliae (strain 789) were shown to cause the death of 100% of the M. alternatus larvae they were exposed to as compared exposures to just one or two infected S. guani females per vial. This demonstrates a successful interaction of two biocontrol agents for the control of the pinewood nematode vector M. alternatus.

Type
Insect Management
Information
The Canadian Entomologist , Volume 147 , Issue 5 , October 2015 , pp. 628 - 634
Copyright
© Entomological Society of Canada 2014 

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Footnotes

Subject editor: Jianghua Sun

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