Skip to main content Accessibility help

Variation in mitochondrial COII gene sequences among two species of Japanese knotweed-boring moths, Ostrinia latipennis and O. ovalipennis (Lepidoptera: Crambidae)

  • S. Ohno (a1), Y. Ishikawa (a1), S. Tatsuki (a1) and S. Hoshizaki (a1)


The Ostrinia latipennis group contains two species, O. latipennis (Warren) and O. ovalipennis Ohno. These two species commonly utilize perennial knotweeds (Fallopia spp.) as their host plants, which are serious invasive weeds in Europe and North America. Ostrinia latipennis is widely distributed across north-east Asia including Japan whereas O. ovalipennis is restricted to north Japan (Hokkaido Is.) and highland areas of central Japan (Nagano Prefecture in Honshu Is.). To estimate the phylogenetic relatedness and geographical differentiation of the two species, mitochondrial COII gene sequences were determined for specimens covering their distribution ranges in Japan. The uncorrected sequence divergence between O. latipennis and O. ovalipennis was 0.6–0.7%, supporting a close relationship. According to the standard molecular clock proposed for arthropod mtDNA, the two species are speculated to have diverged about 0.3 Myr ago. A single COII gene haplotype was found in O. latipennis irrespective of collection locality. In contrast, two haplotypes were found in O. ovalipennis, and their frequencies were significantly different between the Hokkaido and Honshu populations. The patterns of geographical variation in the COII gene within the two species were in agreement with previously reported patterns of geographical differentiation in morphology of the two species in Japan. The present results support the hypothesis that gene flow among local populations of O. ovalipennis has been limited by geographical isolation.


Corresponding author

*Present address: Fruit Fly Eradication Project Office, Okinawa Prefectural Government, Naha, Okinawa 902-0072, Japan Fax: +81 98 884 9119 E-mail:


Hide All
Avise, J.C. (2000) Phylogeography: the history and formation of species. Massachusetts, Harvard University Press.
Ballard, J.W.O. & Whitlock, M.C. (2004) The incomplete natural history of mitochondria. Molecular Ecology 13, 729744.
Ballard, J.W.O., Hatzidakis, J., Karr, T.L. & Kreitman, M. (1996) Reduced variation in Drosophila simulans mitochondrial DNA. Genetics 144, 15191528.
Beerling, D.J., Bailey, J.P. & Conolly, A.P. (1994) Fallopia japonica (Houtt.) Ronse Decraene. Journal of Ecology 82, 959979.
Brower, A.V.Z. (1994) Rapid morphological radiation and convergence among races of the butterfly Heliconius erato inferred from patterns of mitochondrial DNA evolution. Proceedings of the National Academy of Sciences of the United States of America 91, 64916495.
Caterino, M.S., Cho, S. & Sperling, F.A.H. (2000) The current state of insect molecular systematics: a thriving tower of Babel. Annual Review of Entomology 45, 154.
Felsenstein, J. (1993) PHYLIP (phylogeny inference package), version 3.5c Seattle University of Washington
Galtier, N., Depaulis, F. & Barton, N.H. (2000) Detecting bottlenecks and selective sweeps from DNA sequence polymorphism. Genetics 155, 981987.
James, A.C. & Ballard, J.W.O. (2003) Mitochondrial genotype affects fitness in Drosophila simulans. Genetics 164, 187194.
Johnstone, R.A. & Hurst, G.D.D. (1996) Maternally inherited male-killing microorganisms may confound interpretation of mitochondrial DNA variability. Biological Journal of the Linnean Society 58, 453470.
Jukes, T.H. & Cantor, C.R. (1969) Evolution of protein molecules. pp.21132Munroe, H.N. (ed). Mammalian protein metabolism, Vol. III. New York, Academic Press.
Kim, C.-G., Hoshizaki, S., Huang, Y., Tatsuki, S. & Ishikawa, Y. (1999) Usefulness of mitochondrial COII gene sequences in examining phylogenetic relationships in the Asian corn borer, Ostrinia furnacalis, and allied species (Lepidoptera: Pyralidae). Applied Entomology and Zoology 34, 405411.
Landy, B., Powell, J.A. & Sperling, F.A.H. (1999) Systematics of the Argyrotaenia franciscana (Lepidoptera: Tortricidae) species group: evidence from mitochondrial DNA. Annals of the Entomological Society of America 92, 4046.
Liu, H. & Beckenbach, A.T. (1992) Evolution of the mitochondrial cytochrome oxidase II gene among 10 orders of insects. Molecular Phylogenetics and Evolution 1, 4152.
Maynard-Smith, J. & Haigh, J. (1974) The hitch-hiking effect of a favourable gene. Genetical Research, Cambridge 23, 2335.
Mutuura, A. & Munroe, E. (1970) Taxonomy and distribution of the European corn borer and allied species (genus Ostrinia). Memoirs of the Entomological Society of Canada 71, 1112.
Ohno, S. (1998) Geographic variation in morphological characters of the Far Eastern knotweed borer, Ostrinia latipennis (Lepidoptera, Crambidae, Pyraustinae). Transactions of the Lepidopterological Society of Japan 49, 295307. in Japanese with English summary
Ohno, S. (1999) On the genus Ostrinia (Insecta, Lepidoptera, Crambidae) from islands, off Hokkaido (preliminary report). Rishiri Studies 18, 2934. in Japanese with English abstract
Ohno, S. (2000) A case of host expansion in the Far Eastern knotweed borer, Ostrinia latipennis (Lepidoptera, Crambidae, Pyraustinae). Transactions of the Lepidopterological Society of Japan 51, 202204.
Ohno, S. (2001) New records for geographical distributions of the genus Ostrinia (Lepidoptera: Crambidae) in Japan. Japanese Journal of Entomology (New Series) 4, 6364. in Japanese with English abstract
Ohno, S. (2003a) A new knotweed-boring species of the genus Ostrinia Hübner (Lepidoptera: Crambidae) from Japan. Entomological Science 6, 7783.
Ohno, S. (2003b) Systematic study on the Ostrinia latipennis group (Lepidoptera, Crambidae, Pyraustinae) based on morphology, DNA and sex pheromones. PhD dissertation. Tokyo, The University of Tokyo (in Japanese).
Ohno, S., Hoshizaki, S., Ishikawa, Y., Tatsuki, S. & Akimoto, S. (2003a) Allometry of male genitalia in a lepidopteran species, Ostrinia latipennis (Lepidoptera: Crambidae). Applied Entomology and Zoology 38, 313319.
Ohno, S., Hoshizaki, S., Ishikawa, Y. & Tatsuki, S. (2003b) New records of Ostrinia ovalipennis (Lepidoptera: Crambidae) from Hokkaido, and morphometric analysis for species identification and geographic variation. Applied Entomology and Zoology 38, 529535.
Rokas, A., Atkinson, R.J., Brown, G.S., West, S.A. & Stone, G.N. (2001) Understanding patterns of genetic diversity in the oak gallwasp Biorhiza pallida: demographic history or a Wolbachia selective sweep. Heredity 87, 294304.
Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406425.
Serizawa, K., Suzuki, H., Iwasa, M.A., Tsuchiya, K., Pavlenko, M.V., Kartavtseva, I.V., Chelomina, G.N., Dokuchaev, N.E. & Han, S.-H. (2002) A spatial aspect on mitochondrial DNA genealogy in Apodemus peninsulae from East Asia. Biochemical Genetics 40, 149161.
Shaw, R.H. & Seiger, L.A. (2002) Japanese knotweed. pp. 159166 in Van Driesche, R.Biological control of invasive plants in the eastern United States. USDA Forest Service Publication, FHTHT–2002–04.
Shaw, R.H., Evans, H.C., Djeddour, D.H., Tanner, R., Child, L. & Bailey, J.P. (2004) Biological control of Japanese knotweed, Fallopia japonica CABI Bioscience Switzerland Centre Annual Report 2003 27 CABI Bioscience Switzerland Centre
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87, 651701.
Sperling, F.A.H. & Hickey, D.A. (1994) Mitochondrial DNA sequence variation in the spruce budworm species complex (Choristoneura: Lepidoptera). Molecular Biology and Evolution 11, 656665.
Sperling, F.A.H. & Landry, J.-F., Hickey, D.A. (1995) DNA-based identification of introduced ermine moth species in north America (Lepidoptera: Yponomeutidae). Annals of the Entomological Society of America 88, 155162.
Sperling, F.A.H., Byers, R. & Hickey, D.A. (1996) Mitochondrial DNA sequence variation among pheromotypes of the dingy cutworm, Feltia jaculifera (Gn.) (Lepidoptera: Noctuidae). Canadian Journal of Zoology 74, 21092117.
Sperling, F.A.H., Raske, A.G. & Otvos, I.S. (1999) Mitochondrial DNA sequence variation among populations and host races of Lambdina fiscellaria (Gn.) (Lepidoptera: Geometridae). Insect Molecular Biology 8, 97106.
Stouthamer, R., Breeuwer, J.A.J. & Hurst, G.D.D. (1999) Wolbachia pipientis: microbial manipulator of arthropod reproduction. Annual Review of Microbiology 53, 71102.
Templeton, A.R., Crandall, K.A., Sing, C.F. (1992) A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics 132, 619633.
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 46734680.
Zhang, D.-X. & Hewitt, G.M. (1996) Nuclear integrations: challenges for mitochondrial DNA markers. Trends in Ecology and Evolution 11, 247251.
Zhang, D.-X. & Hewitt, G.M. (1997) Insect mitochondrial control region: a review of its structure, evolution and usefulness in evolutionary studies. Biochemical Systematics and Ecology 25, 99120.


Variation in mitochondrial COII gene sequences among two species of Japanese knotweed-boring moths, Ostrinia latipennis and O. ovalipennis (Lepidoptera: Crambidae)

  • S. Ohno (a1), Y. Ishikawa (a1), S. Tatsuki (a1) and S. Hoshizaki (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.