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Universal and differential transcriptional regulatory pathways involved in the preparation of summer and winter diapauses in Pieris melete

Published online by Cambridge University Press:  01 February 2021

Ting Jiang ,
Yulin Zhu ,
Yingchuan Peng ,
Wanna Zhang  and
Haijun Xiao Open the ORCID record for Haijun Xiao [Opens in a new window]
Ting Jiang
Affiliation:
Institute of Entomology, Jiangxi Agricultural University, Nanchang330045, China
Yulin Zhu
Affiliation:
Institute of Entomology, Jiangxi Agricultural University, Nanchang330045, China
Yingchuan Peng
Affiliation:
Institute of Entomology, Jiangxi Agricultural University, Nanchang330045, China
Wanna Zhang
Affiliation:
Institute of Entomology, Jiangxi Agricultural University, Nanchang330045, China
Haijun Xiao*
Affiliation:
Institute of Entomology, Jiangxi Agricultural University, Nanchang330045, China
*
Author for correspondence: Haijun Xiao, Email: hjxiao@jxau.edu.cn
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Abstract

Much progress has been made in understanding the environmental and hormonal systems regulating winter diapause. However, transcriptional regulation of summer diapause is still largely unknown, making it difficult to understand an all-around regulation profile of seasonal adaptation. To bridge this gap, comparison RNA-seq to profile the transcriptome and to examine differential gene expression profiles between non-diapause, summer diapause, and winter diapause groups were performed. A total number of 113 million reads were generated and assembled into 79,117 unigenes, with 37,492 unigenes categorized into 58 functional gene ontology groups, 25 clusters of orthologous group categories, and 256 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. KEGG analysis mapped 2108 differentially expressed genes to 48 and 67 pathways for summer and winter diapauses, respectively. Enrichment statistics showed that 11 identical pathways similarly overlapped in the top 20 enriched functional groups both related to summer and winter diapauses. We also identified 35 key candidate genes for universal and differential functions related to summer and winter diapause preparation. Furthermore, we identified some genes involved in the signaling and metabolic pathways that may be the key drivers to integrate environmental signals into the summer and winter diapause preparation. The current study provided valuable insights into global molecular mechanisms underpinning diapause preparation.

Keywords

Comparative transcriptome analysisdiapause preparationdifferential expressionPieris meletesignal and metabolism pathway
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 111 , Issue 3 , June 2021 , pp. 371 - 378
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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