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Toxicology and genetics of two types of insecticide resistance in Chrysomyia Putoria (Wied.)

  • J. R. Busvine (a1), J. D. Bell (a1) and A. M. Guneidy (a1)


Resistant and normal colonies of the blowfly Chrysomyia putoria (Wied.) were obtained from the Congo and cultured in London. By selection and inbreeding, sub-colonies homogeneous for resistance to (a) the BHC/dieldrin group of chlorinated insecticides and (b) malathion, were obtained. Homogeneous resistant colonies were markedly less fertile and were difficult to perpetuate.

Toxicological investigations of the BHC/dieldrin-resistant colony revealed a characteristic resistance spectrum similar to that of six other insect species. Genetical experiments with the BHC/dieldrin-resistant strain indicated inheritance by a single gene pair, intermediate in dominance, very similar to that reported in nine other insect species.

Toxicological investigations of the malathion-resistant colony, including tests with 19 organophosphorus insecticides, showed a resistance largely specific to malathion and malaoxon. This was rather similar to malathion-resistant strains of two other species, for which data were available. It is known that the toxic action of malathion on mammals can be potentiated by prior treatment with small doses of certain organophosphorus compounds, which inhibit an aliesterase that detoxifies malathion by carboxylic hydrolysis, and furthermore that potentiation has been found in malathion-resistant strains of the house-fly, Musca domestica L. Accordingly, the phenomenon was investigated in C. putoria by adding non-lethal amounts of EPN or tri-o-cresyl phosphate (TOCP) to malathion. It was found that specific malathion-resistance was virtually abolished by this means, a result that suggests that such resistance in this species depends on a carboxyesterase. Genetical experiments with the malathion-resistant strain indicated inheritance by a single gene pair, with nearly complete dominance, similar to that in three other insect species.

It is concluded that both types of resistance in C. putoria have much in common with those occurring in analogous strains of other insect species.



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Bervoets, W., Bruaux, P., Lebrun, A. & Ruzette, M. A. (1958).La lutte contre Chrysomyia putoria à Leopoldville et apparition de phénomènes de résistance.—Mém. Acad. R. Sci. colon. Cl. Sci. nat., 8.° (N.S.) 7 fasc. 4, 53 pp.
Bigley, W. S. & Plapp, F. W. (1962).Metabolism of malathion and malaoxon by the mosquito, Culex tarsalis Coq.—J. Insect Physiol. 8 pp. 545557.
Busvine, J. R. (1959).Patterns of insecticide resistance to organo-phosphorus compounds in strains of house-flies from various sources.—Ent. exp. appl. 2 pp. 5867.
Busvine, J. R. (1962).A laboratory technique for measuring the susceptibility of houseflies and blowflies to insecticides.—Lab. Pract. 11 pp. 464468.
Busvine, J. R, & Townsend, M. G. (1963).The significance of BHC degradation in resistant house-flies.—Bull. ent. Res. 53 pp. 763768.
Darrow, D. I. & Plapp, F. W. (1960).Studies on resistance to malathion in the mosquito, Culex tarsalis.—J. econ. Ent. 53 pp. 777781.
Dauterman, W. C. & Matsumura, F. (1962).Effect of malathion analogs upon resistant and susceptible Culex tarsalis mosquitoes.—Science 138 no. 3541 pp. 694695.
Davidson, G. (1958).Studies on insecticide resistance in Anopheline mosquitos.—Bull. World Hlth Org. 18 pp. 579621.
Davidson, G. & Jackson, C. E. (1961).Insecticide resistance in mosquitoes.—Nature, Lond. 190 no. 4773 pp. 364365.
Forgash, A. J. & Hansens, E. J. (1959).Cross resistance in a diazinon-resistant strain of Musca domestica (L.).—J. econ. Ent. 52 pp. 733739.
Frawley, J. P., Fuyat, H. N., Hagan, E. C., Blake, J. R. & Fitzhugh, O. C. (1957).Marked potentiation in mammalian toxicity from simultaneous administration of two anti-cholinesterase compounds.—J. Pharmacol. 121 pp. 96106.
Guneidy, A. M. (1963). Genetics and toxicology of dieldrin resistance in insects of medical importance.—Ph.D. thesis, Univ. Lond.
Helle, W. (1962).Genetics of resistance to organo-phosphorus compounds and its relation to diapause in Tetranychus urticae Koch (Acari).—Tijdschr. PlZiekt. 68 pp. 155195. (Also Thesis, Univ. Amsterdam.)
Khan, N. H. & Brown, A. W. A. (1961).Genetical studies on dieldrin resistance in Aedes aegypti and its cross-resistance to DDT.—Butt. World Hlth Org. 24 pp. 519526.
Kojima, K. & Ishizuka, T. (1960).The enzymatic detoxification of some organo-phosphorus insecticides in the adults of the green rice leafhopper, Nephotettix bipunctatus cincticeps Uhler, especially an enzyme detoxifying malathion and its inhibition, [In Japanese with English summary.]Botyu-Kagaku 25 pp. 2230.
Matsumura, F. & Brown, A. W. A. (1961).Biochemistry of malathion resistance in Culex tarsalis.–J. econ. Ent. 54 pp. 11761185.
Murphy, S. D., Anderson, R. L. & Dubois, K. P. (1959). Potentiation of toxicity of malathion by triorthotolyl phosphate.—Proc. Soc. exp. Biol., N.Y, 100 pp. 483487.
Nguy, V. D., Busvine, E. & Busvine, J. R. (1960).Studies of the genetics of resistance to parathion and malathion in the housefly.—Bull. World Hlth Org. 22 pp. 531542.
O'Brien, R. D. (1960). Toxic phosphorus esters: chemistry, metabolism and biological effects.New York, Academic Press.
Plapp, F. W. jr. & Eddy, G. W. (1961).Synergism of malathion against resistant insects.—Science 134 no. 3495 pp 20432044.
Seume, F. W. & O'Brien, R. D. (1960).Potentiation of the toxicity to insects and mice of phosphorothionates containing carboxyester and carboxyamide groups.—Toxicol. appl. Pharmacol. 2 pp. 495503.
Shanahan, G. J. (1960).Genetics of resistance to dieldrin in Lucilia cuprina Wied.—Nature, Lond. 186 no. 4719 p. 181.

Toxicology and genetics of two types of insecticide resistance in Chrysomyia Putoria (Wied.)

  • J. R. Busvine (a1), J. D. Bell (a1) and A. M. Guneidy (a1)


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