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Recaptures of feral Bactrocera dorsalis and B. umbrosa (Diptera: Tephritidae) males after feeding on methyl eugenol

  • K.-H. Tan (a1) (a2)


Two major fruit fly pest species, Bactrocera dorsalis and B. umbrosa, are strongly attracted to methyl eugenol (ME) found in >450 plant species. They are, however, exclusive pollinators of certain daciniphilous (attracting Dacini fruit flies) Bulbophyllum orchids. A comparison between the recaptures of feral males after feeding ad libitum on 0.6 mg ME (simulating an average floral quantity of an orchid flower Trial 1) and 480 mg in Trial 2 was investigated using the non-invasive capture-mark-release-recapture (CMRR) technique. Based on daily CMRR over a 16-day period, using a different colour enamel paint each day, percentages of B. dorsalis males recaptured in Trial 1 were significantly higher than those in Trial 2. However, for B. umbrosa, percentages of recaptures for different day-specific colours were highly variable due to low fly numbers captured/day. In Trial 1, of 756 B. dorsalis males released, 36.4% were recaptured once, 7.7 twice, 2.4 three times and 0.4 four times. While in Trial 2 of 1157 released males, 6% were recaptured once and 0.3% twice. Of 67 B. umbrosa males released, 28.4% were recaptured once and none more than once in Trial 1. Nevertheless, of 119 flies released in Trial 2, 25.2% were recaptured once and 3.3% twice. Overall, many marked males did return to a single ME-source to ‘refuel’ ME (a sex pheromone precursor). The results also show that a relatively high number of flies paid multi-visitations to a single 0.6 mg ME-source and indicate that the presence of natural ME-sources may impact area-wide IPM programmes.


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Clarke, A.R., Balagawi, S., Cliffore, B., Drew, R.A.I., Leblanc, L., Mararuai, A., McGuire, D., Putulan, D., Sar, S.A. & Tenakanai, D. (2002) Evidence of orchid visitation by Bactrocera species (Diptera: Tephritidae) in Papua New Guinea. Journal of Tropical Ecology 18, 441448.
Fletcher, B.S. (1987) The biology of Dacine fruit flies. Annual Review of Entomology 32, 115144.
Froerer, K.M., Peck, S.L., McQuate, G.T., Vargas, R.I., Jang, E.B. & McInnis, D. (2010) Long-distance movement of Bactrocera dorsalis (Diptera: Tephritidae) in Puna, Hawaii: how far can they go? American Entomologist 56, 8895.
Hee, A.K.W. & Tan, K.H. (2004) Male sex pheromonal components derived from methyl eugenol in the hemolymph of the fruit fly Bactrocera papayae. Journal of Chemical Ecology 30, 21272138.
Hee, A.K.W. & Tan, K.H. (2006) Transport of methyl eugenol-derived sex pheromonal components in the male fruit fly, Bactrocera dorsalis. Comparative Biochemistry and Physiology Part C 143, 422428.
Hee, A.K.W., Ooi, Y.S., Wee, S.L. & Tan, K.H. (2015a) Comparative sensitivity to methyl eugenol of four putative Bactrocera dorsalis complex sibling species–further evidence that they belong to one and the same species B. dorsalis. ZooKeys 540, 313321.
Hee, A.K.W., Wee, S.L., Nishida, R., Ono, H., Hendrichs, J., Haymer, D.S. & Tan, K.H. (2015b) Historical perspective on the synonymization of the four major pest species belonging to the Bactrocera dorsalis species complex (Diptera, Tephritidae). ZooKeys 540, 323338.
Howlett, F.M. (1915) Chemical reactions of fruit-flies. Bulletin of Entomological Research 6, 297305.
Jang, E.B., Dowell, R.V. & Manoukis, N.C. (2017) Mark-release-recapture experiments on the effectiveness of methyl eugenol-spinosad male annihilation technique against an invading population of Bactrocera dorsalis. Proceedings of the Hawaiian Entomological Society 49, 3745.
Kawano, Y., Mitchell, W.C. & Matsumoto, H. (1968) Identification of the male Oriental fruit fly attractant in the Golden shower blossom. Journal of Economic Entomology 61, 986988.
Khoo, C.C.H. & Tan, K.H. (2005) Rectal gland of Bactrocera papayae: ultrastructure, anatomy, and sequestration of autofluorescent compounds upon methyl eugenol consumption by the male fruit fly. Microscopy Research and Technique 67, 219226.
Khoo, C.C.H., Yuen, K.H. & Tan, K.H. (2000) Attraction of female Bactrocera papayae to sex pheromone components with two different release devices. Journal of Chemical Ecology 26, 24872496.
Manoukis, N.C., Siderhurst, M. & Jang, E.B. (2015) Field estimates of attraction of Ceratitis capitata to trimedlure and Bactrocera dorsalis to methyl eugenol in varying environments. Environmental Entomology 44, 695703.
McInnis, D.O., Hendrichs, J., Shelly, T., Barr, N., Hoffman, K., Rodriguez, R., Lance, D.R., Bloem, K., Suckling, D.M., Enkerlin, W., Gomes, P. & Tan, K.H. (2017) Can polyphagous invasive tephritid pest populations escape detection for years under favorable climatic and host conditions? American Entomologist 63, 8999.
Metcalf, R.L. & Metcalf, E.R. (1992) Plant Kairomones in Insect Ecology and Control. New York/London, Chapman & Hall Inc. 168 pp.
Metcalf, R.L., Mitchell, W.C., Fukuto, T.R. & Metcalf, E.R. (1975) Attraction of the Oriental fruit fly, Dacus dorsalis, to methyl eugenol and related olfactory stimulants. Proceedings of the. National Academy of Science USA 72, 25012502.
Nishida, R., Tan, K.H., Serit, M., Lajis, N.H., Sukari, A.M., Takahashi, S. & Fukami, H. (1988) Accumulation of phenylpropanoids in the rectal glands of males of the Oriental fruit fly, Dacus dorsalis. Experientia 44, 534536.
Nishida, R., Tan, K.H., Wee, S.L., Hee, A.K.W. & Toong, Y.C. (2004) Phenylpropanoids in the fragrance of the fruit fly orchid, Bulbophyllum cheiri, and their relationship to the pollinator, Bactrocera papayae. Biochemical Systematics and Ecology 32, 245252.
Shelly, T. (1994) Consumption of methyl eugenol by male Bactrocera dorsalis (Diptera: Tephritidae): low incidence of repeat feeding. Florida Entomologist 77, 201208.
Shelly, T. (2000) Trapping male oriental fruit flies (Diptera: Tephritidae): does feeding on a natural source of methyl eugenol reduce capture probability? Florida Entomologist 83, 109111.
Shelly, T., Nishimoto, J., Diaz, A., Leathers, J., War, M., Shoemaker, R., Al-Zubaidy, M. & Joseph, D. (2010) Capture probability of released males of two Bactrocera species (Diptera: Tephritidae) in detection traps in California. Journal of Economic Entomology 103, 20422051.
Shelly, T.E., Lance, D.R., Tan, K.H., Sucking, D.M., Bloem, K., Enkerlin, W., Hoffman, K., Barr, K., Rodriguez, R., Gomes, P.J. & Hendrichs, J. (2017) To repeat: can polyphagous invasive tephritid pest populations remain undetected for years under favorable climatic and host conditions? American Entomologist 63, 224231.
Steiner, L.F. (1955) Large-area tests of a male-annihilation method for Oriental fruit fly control. Journal of Economic Entomology 48, 311317.
Tan, K.H. (1985) Estimation of native populations of male Dacus spp. By Jolly's stochastic method using a new designed attractant trap in a village ecosystem. Journal of Plant Protection in the Tropics 2, 8795.
Tan, K.H. (2009) Fruit fly pests as pollinators of wild orchids. Orchid Digest 73, 180187.
Tan, K.H. & Nishida, R. (1996) Sex pheromone and mating competition after methyl eugenol consumption in the Bactrocera dorsalis complex. pp. 147153 in McPheron, B. & Steck, G.J. (Eds) Fruit Fly Pest, Florida, Lucie Press.
Tan, K.H. & Serit, M. (1998) Movements and population density comparisons of native male adult Dacus dorsalis and Dacus umbrosus (Diptera: Tephritidae) among three ecosystems. Journal of Plant Protection in the Tropics 5, 1721.
Tan, K.H. & Nishida, R. (2000) Mutual reproductive benefits between a wild orchid, Bulbophyllum patens, and Bactrocera fruit flies via a floral synomone. Journal of Chemical Ecology 26, 533546.
Tan, K.H. & Nishida, R. (2012) Methyl eugenol: its occurrence, distribution, and role in nature, especially in relation to insect behavior and pollination. Journal of Insect Science 12, 160. https://doi:10.1673/031.012.5601.
Tan, K.H., Nishida, R. & Toong, Y.C. (2002) Floral synomone of a wild orchid, Bulbophyllum cheiri, lures Bactrocera fruit flies for pollination. Journal of Chemical Ecology 28, 11611172.
Tan, K.H., Tan, L.T. & Nishida, R. (2006) Floral phenylpropanoid cocktail and architecture of Bulbophyllum vinaceum orchid in attracting fruit flies for pollination. Journal of Chemical Ecology 32, 24292441.
Tan, K.H., Wee, S.L., Ono, H. & Nishida, R. (2013) Comparison of methyl eugenol metabolites, mitochondrial COI, and rDNA sequences of Bactrocera philippinensis (Diptera: Tephritidae) with those of three other major pest species within the dorsalis complex. Applied Entomology and Zoology 48, 275282.
Tan, K.H., Nishida, R., Jang, E.B. & Shelly, T. (2014) Pheromones, male lures, and trapping of tephritid fruit flies. pp. 1574 in Shelly, T., Epsky, N., Jang, E.B., Reyes-Flores, J. & Vargas, R. (Eds) Trapping and the Detection, Control, and Regulation of Tephritid Fruit Flies. The Netherlands, Springer, Science + Business Media Dordrecht.
Wee, S.L. & Tan, K.H. (2001) Allomonal and hepatotoxic effects following methyl eugenol consumption in Bactrocera papayae male against Gekko monarchus. Journal of Chemical Ecology 27, 953964.
Wee, S.L. & Tan, K.H. (2007) Temporal accumulation of phenylpropanoids in male fruit flies, Bactrocera dorsalis and B. carambolae (Diptera: Tephritidae) following methyl eugenol consumption. Chemoecology 17, 8185.
Wee, S.L., Hee, A.K.W. & Tan, K.H. (2002) Comparative sensitivity to and consumption of methyl eugenol in three Bactrocera dorsalis (Diptera: Tephritidae) complex sibling species. Chemoecology 12, 193197.
Wee, S.L., Chinvinijkul, S., Tan, K.H. & Nishida, R. (2018 a) A new and highly effective male lure for the guava fruit fly Bactrocera correcta. Journal of Pest Science 91, 691698.
Wee, S.L., Abdul Munir, M.Z. & Hee, A.K.W. (2018 b) Attraction and consumption of methyl eugenol by male Bactrocera umbrosa Fabricius (Diptera: Tephritidae) promotes conspecific sexual communication and mating performance. Bulletin of Entomological Research 108, 116124.


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Recaptures of feral Bactrocera dorsalis and B. umbrosa (Diptera: Tephritidae) males after feeding on methyl eugenol

  • K.-H. Tan (a1) (a2)


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