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Random amplified polymorphic DNA markers for discriminating Cochliomyia hominivorax from C. macellaria (Diptera: Calliphoridae)

Published online by Cambridge University Press:  09 March 2007

S.R. Skoda ,
S. Pornkulwat  and
J.E. Foster
S.R. Skoda*
Affiliation:
USDA-ARS-MLIRU, University of Nebraska, Lincoln, NE 68583-0938, USA
S. Pornkulwat
Affiliation:
Department of Entomology, Insect Genetics Laboratory, University of Nebraska, Lincoln, NE 68583-0816, USA
J.E. Foster
Affiliation:
Department of Entomology, Insect Genetics Laboratory, University of Nebraska, Lincoln, NE 68583-0816, USA
*
*Fax: (402) 437-5260 E-mail: sskoda1@unl.edu
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Abstract

The screwworm, Cochliomyia hominivorax (Coquerel), is one of the most important pests of livestock in the Western Hemisphere. During early immature stages it is morphologically very similar (first instars are virtually indistinguishable) to the secondary screwworm, C. macellaria (Fabricius). Here, the utility of the random amplified polymorphic DNA–polymerase chain reaction (RAPD–PCR) was explored as a technique for developing molecular genetic markers for these two species. Of the 120 arbitrary primers screened, 21 primers produced markers that were further investigated. Seven of the 21 primers produced clear and reproducible markers that were tested with DNA of five individuals from four populations of each species; five of these primers showed 12 RAPD markers that differentiated the species in all populations. Analyses of data from these seven primers also suggested that intraspecific polymorphisms exist that could be useful in distinguishing populations of screwworms. Some population genetic tools, such as genetic distance, cluster analysis and bootstrapping, were used to statistically explore these polymorphisms. The resulting statistics showed 100% support for the ability of RAPD–PCR to discriminate between the two species. Bootstrapping with data from one of the genetic distance calculations produced a tree with all individual screwworms in the correct populations, indicating that RAPD–PCR has promise for displaying intraspecific genetic variation that could be used in establishing the general geographic origin of screwworm samples.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 92 , Issue 1 , February 2002 , pp. 89 - 96
Copyright
Copyright © Cambridge University Press 2002

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References

Apostol, B.L., Black, W.C. IV, Miller, B.R., Reiter, P. & Beaty, B.J. (1993) Estimation of the number of full sibling families at an ovipositon site using RAPD–PCR markers: applications to the mosquito Aedes aegypti. Theoretical and Applied Genetics 86, 991–1000.CrossRefGoogle Scholar
Apostol, B.L., Black, W.C. IV, Reiter, P. & Miller, B.R. (1996) Population genetics with RAPD–PCR markers: the breeding structure of Aedes aegypti in Puerto Rico. Heredity 76, 325–334.CrossRefGoogle ScholarPubMed
Black, W.C. (1993) PCR with arbitrary primers: approach with care. Insect Molecular Biology 2, 1–6.CrossRefGoogle ScholarPubMed
Black, W.C. IV, Duteau, N.M., Puterka, G.J., Nicols, J.R. & Pettorini, J.M. (1992) Use of the random amplified polymorphic DNA polymerase chain reaction (RAPD–PCR) to detect DNA polymorphisms in aphids (Homoptera: Aphididae). Bulletin of Entomological Research 82, 151–159.CrossRefGoogle Scholar
Cushing, E.E. & Patton, W.S. (1933) Studies on the higher Diptera of medical and veterinary importance: Cochliomyia americana sp. nov., the screwworm fly of the New World. Annals of Tropical Medicine and Parasitology 27, 539–551.CrossRefGoogle Scholar
Dear, J.P. (1985) A revision of the new world Chrysomyini (Diptera: Calliphoridae). Revista Brasileira de Zoologia 3, 109–169.CrossRefGoogle Scholar
FAO (1992) The New World screwworm eradication programme: North Africa 1988–1992. Food and Agriculture Organization of the United Nations, Rome, 1992.Google Scholar
Felsenstein, J. (2000) PHYLIP (Phylogeny Inference Package) version 3.6 (alpha). Distributed by the author, Department of Genetics, University of Washington, Seattle.Google Scholar
Hall, D.G. (1948) The blowflies of North America. Thomas Say Foundation, Entomological Society of America, Lanham, Maryland.Google Scholar
Haymer, D.S. (1994) Random amplified polymorphic DNA and microsatellites. Annals of the Entomological Society of America 87, 717–722.CrossRefGoogle Scholar
Hoy, M.A. (1994) Insect molecular genetics: an introduction to principles and applications. San Diego, California, Academic Press.CrossRefGoogle Scholar
Hunt, G.J. & Page, R.E. (1995) Linkage map of the honey bee, Apis mellifera, based on RAPD markers. Genetics 139, 1371–1382.CrossRefGoogle ScholarPubMed
Infante-Malachias, M.E., Yotoko, K.S.C. & Azeredo-Espin, A.M.L. (1999) Random amplified polymorphic DNA of screwworm fly populations (Diptera: Calliphoridae) from southeastern Brazil and northern Argentina. Genome 42, 772–779.CrossRefGoogle Scholar
Infante-Vargas, M.E. & Azeredo-Espin, A.M.L. (1995) Genetic variability in mitochondrial DNA of the screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), from Brazil. Biochemical Genetics 33, 237–256.CrossRefGoogle Scholar
Knipling, E.F. (1939) A key for blowfly larvae concerned in wound and cutaneous myiasis. Annals of the Entomological Society of America 32, 376–383.CrossRefGoogle Scholar
Leite, A.C.R. & Guevara, J.D.E. (1993) Scanning electron microscopy of the larval instars of Cochliomyia hominivorax. Medical and Veterinary Entomology 7, 263–270.CrossRefGoogle ScholarPubMed
Lynch, M. & Milligan, B.G. (1994) Analysis of population genetic structure with RAPD markers. Molecular Ecology 3, 91–99.CrossRefGoogle ScholarPubMed
McClelland, M. & Welsh, J. (1995) DNA fingerprinting using arbitrary primed PCR. pp. 203–212 in Dieffenbach, C.W. & Dveksler, G.S. (Eds) PCR primer: a laboratory manual. New York, Cold Spring Harbor Laboratory Press.Google Scholar
Metcalf, R.L. & Metcalf, R.A. (1993) Destructive and useful insects. New York, McGraw Hill.Google Scholar
Nei, M. (1972) Genetic distance between populations. American Naturalist 106, 283–292.CrossRefGoogle Scholar
Nei, M. & Li, W.H. (1985) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences USA 76, 5269–5273.CrossRefGoogle Scholar
Pornkulwat, S., Skoda, S.R., Thomas, G.D. & Foster, J.E. (1998) Random amplified polymorphic DNA used to identify genetic variation in ecotypes of the European corn borer (Lepidoptera: Pyralidae). Annals of the Entomological Society of America 91, 719–725.CrossRefGoogle Scholar
Rafalski, J.A. & Tingey, S.V. (1993) Genetic diagnostics in plant breeding: RAPDs, microsatellites and machines. Trends in Genetics 9, 270–280.Google ScholarPubMed
Roderick, G.K. (1996) Geographic structure of insect populations: gene flow, phylogeography, and their uses. Annual Review of Entomology 41, 325–352.CrossRefGoogle ScholarPubMed
Roehrdanz, R.L. (1989) Intraspecific genetic variability in mitochondrial DNA of the screwworm fly (Cochliomyia hominivorax). Biochemical Genetics 27, 551–569.CrossRefGoogle Scholar
Roehrdanz, R.L & Johnson, D.A. (1988) Mitochondrial DNA variation among geographical populations of the screwworm fly, Cochliomyia hominivorax. Journal of Medical Entomology 25, 136–141.CrossRefGoogle ScholarPubMed
Taylor, D.B. & Peterson, R.D. II (1994) Population genetics and gene variation in primary and secondary screwworm (Diptera: Calliphoridae). Annals of the Entomological Society of America 87, 626–633.CrossRefGoogle Scholar
Taylor, D.B., Peterson, R.D. II & Moya-Borja, G.E. (1996) Population genetics and gene variation in screwworms (Diptera: Calliphoridae) from Brazil. Biochemical Genetics 34, 67–76.CrossRefGoogle ScholarPubMed
Taylor, D.B., Szalanski, A.L. & Peterson, R.D. II (1996) Identification of screwworm species by polymerase chain reaction-restriction fragment length polymorphism. Medical and Veterinary Entomology 10, 63–70.CrossRefGoogle ScholarPubMed
Taylor, D.B., Szalanski, A.L. & Peterson, R.D. II (1996) Mitochondrial DNA variation in screwworm. Medical and Veterinary Entomology 10, 161–169.CrossRefGoogle ScholarPubMed
Welsh, J. & McClelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18, 7213–7218.CrossRefGoogle ScholarPubMed
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. & Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531–6535.CrossRefGoogle ScholarPubMed
Wyss, J.H. & Galvin, T.J. (1996) Central America regional screwworm eradication program (benefit/cost study). Annals of the New York Academy of Sciences 791, 241–247.CrossRefGoogle Scholar