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Purified winged bean protease inhibitor affects the growth of Bactrocera cucurbitae

Published online by Cambridge University Press:  20 December 2018

A.P. Kaur  and
S.K. Sohal
A.P. Kaur
Affiliation:
Department of Zoology, Guru Nanak Dev University, Amritsar 143 005, India
S.K. Sohal*
Affiliation:
Department of Zoology, Guru Nanak Dev University, Amritsar 143 005, India
*
*Author for correspondence Phone: 91-183-2258802-09; Extn. 3398 Fax: 91-183-2258819 E-mail: satudhillon27@hotmail.com
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Abstract

The melon fruit fly, Bactrocera cucurbitae (Coquillett), is a serious agricultural pest which has defied the various control measures employed against it. Protease inhibitors present in plants which have the potential to inhibit the growth and development of associated insect pests can be a possible alternative which can be manipulated for developing resistance in plants to the pest. In the present study, winged bean (Psophocarpus tetragonolobus) protease inhibitor isolated through affinity chromatography was explored for its potential to disrupt the development of melon fruit fly, B. cucurbitae. Different concentrations (12.5, 25, 50, 100, 200, and 400 µg ml−1) of the winged bean protease inhibitor (WBPI) were incorporated into the artificial diet of the second instar (64–72 h old) larvae of B. cucurbitae. The WBPI significantly delayed the larval, pupal, and total development period. The percentage pupation and adult emergence of the treated larvae was reduced as compared with control. The activities of major digestive enzymes (trypsin, chymotrypsin, leucine aminopeptidase, and elastase) decreased significantly in the larvae treated with different concentrations (50, 100, 200, and 400 µg ml−1) of WBPI. The findings reveal that the inhibitor holds considerable promise for the management of the melon fruit fly.

Keywords

Bactrocera cucurbitaePsophocarpus tetragonolobusmelon fruit flyprotease inhibitorswinged beans
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 109 , Issue 4 , August 2019 , pp. 550 - 558
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Copyright
Copyright © Cambridge University Press 2018 

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