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Larval species composition and genetic structures of Carposina sasakii, Grapholita dimorpha, and Grapholita molesta from Korea

Published online by Cambridge University Press:  31 July 2017

D.H. Kwon ,
H.K. Kwon ,
D.H. Kim  and
C.Y. Yang
D.H. Kwon*
Affiliation:
Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea
H.K. Kwon
Affiliation:
Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeollabuk-do 55365, Republic of Korea
D.H. Kim
Affiliation:
Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeollabuk-do 55365, Republic of Korea
C.Y. Yang*
Affiliation:
Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeollabuk-do 55365, Republic of Korea
*
*Author for correspondence Phone: +01182-2-880-4827 (D.H. Kwon), +01182-63-238-6331 (C.Y. Yang) Fax: +01182-2-873-2319 (D.H. Kwon), +01182-63-238-6305 (C.Y. Yang) E-mail: jota486486@snu.ac.kr (D.H. Kwon) and cyyang@korea.kr (C.Y. Yang)
*Author for correspondence Phone: +01182-2-880-4827 (D.H. Kwon), +01182-63-238-6331 (C.Y. Yang) Fax: +01182-2-873-2319 (D.H. Kwon), +01182-63-238-6305 (C.Y. Yang) E-mail: jota486486@snu.ac.kr (D.H. Kwon) and cyyang@korea.kr (C.Y. Yang)
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Abstract

Rapid determination of the larval species composition and understanding of their genetic structure is important to establish the appropriate management system for multiple species infesting in fruits. We established accurate and rapid diagnostic methods based on multiplex polymerase chain reaction (PCR) diagnostic techniques to discriminate the three major lepidopteran species in orchard, Carposina sasakii, Grapholita dimorpha, and Grapholita molesta. Each species was identified by amplifying species-specific PCR products (375 bp for C. sasakii, 125 and 234 bp for G. dimorpha, and 125 bp for G. molesta). Based on species composition analysis from six types of infested fruits, G. dimorpha constituted the highest proportion (47.8%), followed by 35.2 and 13.5% for G. molesta and C. sasakii, respectively. Interestingly, high prevalence was found in G. dimorpha and G. molesta for plum and peach, respectively. Based on genetic diversity analysis, the three insect species exhibited moderate or high haplotype diversity and low nucleotide diversity, ranging from 0.319 to 0.699 and 0.0006 to 0.0045, respectively. Demographic expansion was not detected according to either a neutrality test or mismatch distribution analysis. Moreover, no significant genetic structure corresponding to province, host plant, fruit type, or collection period was observed. These results suggest that the population of each species would have high dispersal ability following fruit-generating periods via intrinsic host adaptation ability regardless of the spatial and temporal conditions. Determination of larval composition on fruit is valuable for establishing appropriate management systems that take the species into consideration; additionally, population genetic approaches can be utilized to understand the effects of environmental factors (province, host fruit, fruit type, etc.) on population structures.

Keywords

species diagnosismultiplex PCRCarposina sasakiiGrapholita dimorphaGrapholita molestagenetic structure
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 2 , April 2018 , pp. 241 - 252
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Copyright
Copyright © Cambridge University Press 2017 

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References

Ahn, S.-J., Choi, K.-H., Kang, T.J., Kim, H.H., Kim, D.-H., Cho, M.R. & Yang, C.Y. (2013) Molecular diagnosis of Grapholita molesta and Grapholita dimorpha and their different occurrence in peach and plum. Korean Journal of Applied Entomology 52, 365–370.CrossRefGoogle Scholar
Barcenas, N.M., Unruh, T.R. & Neven, L.G. (2005) DNA diagnostics to identify internal feeders (Lepidoptera: Tortricidae) of pome fruits of quarantine importance. Journal of Economic Entomology 98, 299–306.CrossRefGoogle ScholarPubMed
Beljaev, E. & Ponomarenko, M. (2005) New lepidopterological finds (Lepidoptera: Gelechiidae, Tortricidae, Geometridae) in south of Russian Far East. Far Eastern Entomologist 155, 1–11.Google Scholar
Chaudhry, G.-U. (1956) The development and fecundity of the oriental fruit moth, Grapholitha (Cydia) molesta (Busck) under controlled temperatures and humidities. Bulletin of Entomological Research 46, 869–898.CrossRefGoogle Scholar
Chen, M. & Dorn, S. (2009) Reliable and efficient discrimination of four internal fruit-feeding Cydia and Grapholita species (Lepidoptera: Tortricidae) by polymerase chain reaction-restriction fragment length polymorphism. Journal of Economic Entomology 102, 2209–2216.CrossRefGoogle ScholarPubMed
Choi, K.-H., Lee, S.-W., Lee, D.-H., Kim, D.-A. & Kim, S.-K. (2008) Recent occurrence status of two major fruit moths, oriental fruit moth and peach fruit moth in apple orchards. Korean Journal of Applied Entomology 47, 17–22.CrossRefGoogle Scholar
Choi, K.-H., Lee, D.-H., Byun, B.-K. & Mochizuki, F. (2009) Occurrence of Grapholita dimorpha Komai (Lepidoptera: Tortricidae), a new insect pest in apple orchards of Korea. Korean Journal of Applied Entomology 48, 417–421.CrossRefGoogle Scholar
Choi, K.S., Lee, S.-Y., Choi, K.-H., Jang, I., Kang, I.-K. & Jung, H.-Y. (2016) Simultaneous identification of Grapholita molesta Busck and Grapholita dimorpha Komai by PCR-RFLP. Entomological Research 46, 206–209.CrossRefGoogle Scholar
Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 1792–1797.CrossRefGoogle ScholarPubMed
Excoffier, L. & Lischer, H.E. (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources 10, 564–567.CrossRefGoogle ScholarPubMed
Folmer, O., Hoeh, W., Black, M. & Vrijenhoek, R. (1994) Conserved primers for PCR amplification of mitochondrial DNA from different invertebrate phyla. Molecular Marine Biology and Biotechnology 3, 294–299.Google Scholar
Hada, H. & Sekine, K.-T. (2011) A diagnostic multiplex polymerase chain reaction method to identify Japanese internal apple-feeding Lepidopteran pests: Grapholita molesta, Grapholita dimorpha (Lepidoptera: Tortricidae), and Carposina sasakii (Lepidoptera: Carposinidae). Applied Entomology and Zoology 46, 287–291.CrossRefGoogle Scholar
Ishiguri, Y. & Shirai, Y. (2004) Flight activity of the peach fruit moth, Carposina sasakii (Lepidoptera: Carposinidae), measured by a flight mill. Applied Entomology and Zoology 39, 127–131.CrossRefGoogle Scholar
Jeong, S.A., Sah, L.P., Ahn, J.J., Kim, Y.I. & Jung, C. (2012) Occurrence patterns of three major fruit moths, Grapholita molesta, Grapholita dimorpha and Carposina sasakii, monitored by sex pheromone in plum orchards. Korean Journal of Applied Entomology 51, 449–459.CrossRefGoogle Scholar
Jung, C.R. & Kim, Y. (2013) Different types of fruit damages of three internal apple feeders diagnosed with mitochondrial molecular markers. Journal of Asia-Pacific Entomology 16, 189–197.CrossRefGoogle Scholar
Kim, D.-S., Lee, J.-H. & Yiem, M.-S. (2000) Spring emergence pattern of Carposina sasakii (Lepidoptera: Carposinidae) in apple orchards in Korea and its forecasting models based on degree-days. Environmental Entomology 29, 1188–1198.CrossRefGoogle Scholar
Kim, D.-S., Lee, J.-H. & Yiem, M.-S. (2001) Temperature-dependent development of Carposina sasakii (Lepidoptera: Carposinidae) and its stage emergence models. Environmental Entomology 30, 298–305.CrossRefGoogle Scholar
Kim, Y., Bae, S., Son, Y. & Park, J. (2009) Analysis of migration of the oriental fruit moth, Grapholita molesta, in apple-cultivating areas based on population monitoring using sex pheromone and RAPD molecular marker. Korean Journal of Applied Entomology 48, 211–219.CrossRefGoogle Scholar
Kirk, H., Dorn, S. & Mazzi, D. (2013) Worldwide population genetic structure of the oriental fruit moth (Grapholita molesta), a globally invasive pest. BMC Ecology 13, 12.CrossRefGoogle ScholarPubMed
Komai, F. (1979) A new species of the genus Grapholita Treitschke from Japan allied to the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae). Applied Entomology and Zoology 14, 133–136.CrossRefGoogle Scholar
Komai, F. (1999) A taxonomic review of the genus Grapholita and allied genera (Lepidoptera: Tortricidae) in the Palaearctic region. Scandinavian Entomology 55, 1–226.Google Scholar
Kwon, D.H., Kim, D.H., Kim, H.H., Lee, S.H. & Yang, C.Y. (2017) Genetic diversity and structure in apple-infesting pests of Carposina sasakii, Grapholita dimorpha and Grapholita molesta in Korea. Journal of Asia-Pacific Entomology 20, 13–16.CrossRefGoogle Scholar
Lee, S.-Y., Choi, K.-S., Choi, K.-H., Yoon, T.-M. & Jung, H.-Y. (2013) Morphological differences between larvae of the oriental fruit moth (Grapholita molesta Busck) and the peach fruit moth (Carposina sasakii Matsumura) in Korea. Applied Microscopy 43, 21–26.CrossRefGoogle Scholar
Lee, S.Y., Choi, K.S., Back, C.G., Choi, K.H., Kang, I.K., Jung, H.Y. & Ohga, S. (2015) Distinction of Grapholita molesta Busck and Grapholita dimorpha Komai larvae based on morphological feature of anal prolegs. Faculty of Agriculture Kyushu University 60, 291–295.CrossRefGoogle Scholar
Librado, P. & Rozas, J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 1451–1452.CrossRefGoogle ScholarPubMed
Liu, Y., Cheng, J. & Mou, J. (1997) Review of the advances of the peach fruit-borer (Carposina sasakii Matsumura). Journal of Shandong Agricultural University 28, 207–214.Google Scholar
Ma, T., Li, Y., Sun, Z. & Wen, X. (2014) (Z,E)-9,12-Tetradecadien-1-Ol: a major sex pheromone component of Euzophera pyriella (Lepidoptera: Pyralididae) in Xinjiang, China. Florida Entomologist 97, 496–503.CrossRefGoogle Scholar
Machida, T. & Aoyama, A. (1931) Pests in Korea: Pests of Fruit Trees. Tokyo, Ext Lite Hal, pp. 53–57.Google Scholar
Makaji, T. (1987) Diagnosis of Fruit Tree Diseases and Pests with Color Plates. Tokyo, Agriculture Publication Incorporation.Google Scholar
Oku, T., Ohira, Y. & Wakou, M. (1988) Preliminary notes on a plum [Prunus salicina] fruit moth, Grapholita dimorpha Komai (Lepidoptera: Tortricidae). Bulletin of the Fruit Trees Research Station Series C, Morioka 15, 49–64.Google Scholar
Park, K.-T. & Kim, J.-M. (1986) Moths collected in the northen part of civilian control line neighbouring DMZ. Korean Journal of Applied Entomology 25, 77–83.Google Scholar
Phillips, J. & Proctor, J.R. (1970) Development of methods for sampling the oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae), in an Ontario peach orchard. Canadian Entomologist 102, 454–471.CrossRefGoogle Scholar
Pree, D., Trimble, R., Whitty, K. & Vickers, P. (1994) Control of oriental fruit moth by mating disruption using sex pheromone in the Niagara Peninsula, Ontario. Canadian Entomologist 126, 1287–1299.CrossRefGoogle Scholar
Song, M.J., Zhou, L.N., Zai, Y.D., Zhang, X., Xing, H.T., Li, S.F. & Xiao, F. (1994) A preliminary study of Euzophera pyriella . Plant Protection 20, 13–15.Google Scholar
Song, S.-B., Choi, K.-H., Lee, S.-W. & Kim, Y.-G. (2007) DNA markers applicable for identification of two internal apple feeders, Grapholita molesta and Carposina sasakii . Korean Journal of Applied Entomology 46, 175–182.CrossRefGoogle Scholar
Sun, J.-T., Wang, M.-M., Zhang, Y.-K., Chapuis, M.-P., Jiang, X.-Y., Hu, G., Yang, X.-M., Ge, C., Xue, X.-F. & Hong, X.-Y. (2015) Evidence for high dispersal ability and mito-nuclear discordance in the small brown planthopper, Laodelphax striatellus . Scientific Reports 5, 8045.CrossRefGoogle ScholarPubMed
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729.CrossRefGoogle ScholarPubMed
Tikhonov, N.P. (1962) Orchard fruit moths of the Far east. Zashchita Rastenii 7, 45–46.Google Scholar
Wang, J., Yu, Y., Li, L.-L., Guo, D., Tao, Y.-L. & Chu, D. (2015) Carposina sasakii (Lepidoptera: Carposinidae) in its native range consists of two sympatric cryptic lineages as revealed by mitochondrial COI gene sequences. Journal of Insect Science 15, 85.CrossRefGoogle ScholarPubMed
Wei, S.J., Cao, L.J., Gong, Y.J., Shi, B.C., Wang, S., Zhang, F., Guo, X.J., Wang, Y.M. & Chen, X.X. (2015) Population genetic structure and approximate Bayesian computation analyses reveal the southern origin and northward dispersal of the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae) in its native range. Molecular Ecology 24, 4094–4111.CrossRefGoogle ScholarPubMed
Yan, S., Liu, Y. & Li, M. (1998) Grapholita dimorpha-a new record pest damaging fruit trees of China. Forest Pest and Disease 18, 15–16.Google Scholar
Yang, C., Han, K. & Boo, K. (2001) Occurrence of and damage by the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) in pear orchards. Korean Journal of Applied Entomology 40, 117–124.Google Scholar
Yang, C.Y., Han, K.S., Jung, J.K., Boo, K.S. & Yiem, M.S. (2003) Control of the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) by mating disruption with sex pheromone in pear orchards. Journal of Asia-Pacific Entomology 6, 97–104.CrossRefGoogle Scholar
Zheng, Y., Peng, X., Liu, G., Pan, H., Dorn, S. & Chen, M. (2013) High genetic diversity and structured populations of the oriental fruit moth in its range of origin. PLoS ONE 8, e78476.CrossRefGoogle ScholarPubMed
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