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Honeydew sugars in wild-caught Italian phlebotomine sandflies (Diptera: Psychodidae) as detected by high performance liquid chromatography

  • Jane A.K. MacVicker (a1), J.S. Moore (a1), D.H. Molyneux (a1) and M. Maroli (a2)

Abstract

Phlebotomus perfiliewi perfiliewi Parrott and Phlebotomus perniciosus Newstead (Diptera: Psychodidae,) known vectors of Leishmania infantum were caught in light traps in five different sites in Italy. Sandflies were cryopreserved and transported to the laboratory where they were thawed and aqueous extracts of the crushed flies were analysed for their carbohydrate content using high performance liquid chromatography. Several sugars were detected, including significant amounts of the trisaccharide melezitose, known to be available as a natural source from aphid or coccid honeydew. There was no difference between the analyses of sugar meals of P. p. perfiliewi from different sites within its range, or between P. p. perfiliewi and P. perniciosus. The results indicate that both males and females of P. p. perfiliewi and P. perniciosus feed on honeydew.

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Adler, S. & Theodor, O. (1957) Transmission of disease agents by phlebotomine sandflies. Annual Review of Entomology 2, 203226.
Ashford, R.W. (1974) Sandflies (Diptera: Phlebotomidae) from Ethiopia: taxonomic and biological notes. Journal of Medical Entomology 11, 605616.
Auclair, J.L. (1963) Aphid feeding and nutrition. Annual Review of Entomology 8, 439490.
Bacon, J.S.D. & Dickinson, B. (1957) The origin of melezitose: a biochemical relationship between the lime tree (Tilia spp.) and an aphis (Eucallipterus tiliae L.). Biochemistry Journal 66, 289299.
Belliardo, F., Lombard, A., Patetta, A. & Vidano, C. (1978) Indagini sui cardoidrati presenti in foglie di Tilia cordata, melata di Eucallypterus tiliae e relative miele. Apicoltore Moderno 69, 512.
Bettini, S., Gramiccia, M., Gradoni, L. & Atzeni, M.C. (1986) Leishmaniasis in Sardinia. II. Natural infection of Phlebotomus perniciosus Newstead, 1911, by Leishmania infantum Nicolle, 1908, in the province of Cagliari. Transactions of the Royal Society of Tropical Medicine and Hygiene 80, 458459.
Blackwell, J.M. (1985) Receptors and recognition mechanisms of Leishmania species. Transactions of the Royal Society of Tropical Medicine and Hygiene 79, 606612.
Bray, R.S. (1983) Chemotaxic responses of promastigotes and macrophages in vitro. Journal of Protozoology 30, 322329.
Chaniotis, B.N. (1974) Sugar feeding behaviour of Lutzomyia trapidoi (Diptera: Psychodidae) under experimental conditions. Journal of Medical Entomology 11, 7379.
Corradetti, A. (1962) Phlebotomus and leishmaniasis in northcentral Italy (Apennine Region). Scientific Reports of the Institute Superiore di Sanita 2, 103109.
Dixon, A.F.G. (1985) Aphid ecology. 157 pp. Glasgow, Blackie.
Ewart, W.H. & Metcalf, R.L. (1956) Preliminary studies of sugars and amino acids in the honeydews of five species of coccids feeding on citrus in California. Annals of the Entomological Society of America 49, 441447.
Gramiccia, M., Gradoni, L. & Pozio, E. (1987) Leishmania infantum sensu lato as an agent of cutaneous leishmaniasis in Abruzzi region (Italy). Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 235237.
Gray, H.E. & Fraenkel, G. (1953) Fructomaltose, a recently discovered trisaccharide isolated from honeydew. Science 118, 304305.
Ibrahim, E.A.R., Ingram, G.A. & Molyneux, D.H. (1984) Haemagglutins and parasite agglutins in haemolymph and gut of Glossina. Tropenmedizin und Parasitologie 35, 151156.
Kandler, O. & Hopf, H. (1980) Occurrence, metabolism and function of oligosacchardies. p. 221270in Peiss, J. (Ed.) The biochemistry of plants. New York, Academic Press.
Killick-Kendrick, R. (1979) The biology of Leishmania in phlebotomine sandflies. pp. 395460in Lumsden, W.H.R. & Evans, D.A. (Eds) Biology of kinetoplastida. Vol. II London, Academic Press.
Killick-Kendrick, R. & Killick-Kendrick, M. (1987) Honeydew of aphids as a source of sugar for Phlebotomus ariasi. Medical and Veterinary Entomology 1, 297302.
Lewis, D.J. & Domoney, C.R. (1966) Sugar meals in Phlebotominae and Simulidae. Proceedings of the Royal Entomological Society of London A 41, 175179.
Lombard, A., Buffa, M., Manino, A. & Patetta, A. (1988) Composizione glucidica di metata di Thelaxes dryophila (Sch.) Apicoltore Moderno 79, 36.
Lombard, A., Buffa, M., Patetta, A., Manino, A. & Marletto, F. (1987) Some aspects of the carbohydrate composition of calliphorid honeydew. Journal of Apiculture Research 26, 233237.
Manino, A., Patetta, A., Marletto, F., Lombard, A. & Buffa, M. (1985) Sequential carbohydrate variations from larch phloem sap to honeydew and to honeydew honey. Apicoltore Moderno 1, 93204.
Maroli, M. & Bettini, S. (1977) Leishmaniasis in Tuscany (Italy): (I) An investigation on phlebotomine sandflies in Grosseto Province. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 315321.
Maroli, M., Gramiccia, M. & Gradoni, L. (1987) Natural infection of Phlebotomus perfiliewi with Leishmania infantum in a cutaneous leishmaniasis focus of the Abruzzi region, Italy. Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 596598.
Maroli, M., Gramicca, M., Gradoni, L., Ready, P.D., Smith, D.F. & Aquino, C. (1988) Natural infections of phlebotomine sandflies with Trypanosomatidae in central south Italy. Transactions of the Royal Society of Tropical Medicine and Hygiene 82, 227228.
Molyneux, D.H., Ryan, L., Lainson, R. & Shaw, J.J. (1986) The Leishmania-sandfly interface. Leishmania. Taxonomie et phylogenèse. Applications eco-epidemiologiques (Coll.int. CNRS/INSERM, 1984) IMEEE, Montpellier 311–324.
Moore, J.S., Kelly, T.B., Killick-Kendrick, R., Killick-Kendrick, M., Wallbanks, K.R. & Molyneux, D.H. (1987) Honeydew sugars in wild-caught Phlebotomus ariasi detected by high performance liquid chromatogrpahy (HPLC) and gas chromatography (GC). Medical and Veterinary Entomology 1, 427434.
Pozio, E., Gradoni, L., Bettini, S. & Gramiccia, M. (1981) Leishmaniasis in Tuscany (Italy). (V). Further isolation of Leishmania from Rattus rattus in the province of Grosseto. Annals of Tropical Medicine and Parasitology 75, 393395.
Sacks, D.L. & Perkins, P.V. (1985) Development of infective stage Leishmania promastigotes within phlebotomine sandflies. American Journal of Tropical Medicine and Hygiene 34, 456459.
Schlein, Y., Borut, S. & Greenblatt, C.OL. (1987) Development of sandfly forms of Leishmania major in sucrose solutions. Journal of Parasitology 73, 797805.
Schlein, Y. & Warburg, A. (1986) Phytophagy and the feeding cycle of Phlebotomus papatasi (Dipera: Psychodidae) under experimental conditions. Journal of Medical Entomology 23, 1115.
Trager, W. (1974) Nutrition and biosynthetic capabilities of flagellates: problems of ‘in vitro’ cultivaton and differentiation. pp. 225254in Trypanosomiasis and leishmaniasis with special reference to Chagas disease. Ciba Foundation Symposium 20 (new series), Amsterdam, Associated Scientific Publishers.
Van Handel, E. (1972) The detection of nectar in mosquitoes. Mosquito News 32, 458.
Wallbanks, K.R., Ingram, G.A. & Molyneux, D.H. (1986) The agglutination of erythrocytes and Leishmania parasites by sandfly gut extracts: evidence for lectin activity. Tropical Medicine and Parasitology 37, 409413.
Welburn, S.L., Maudlin, I. & Ellis, D.S. (1989) Rate of trypanosome killing of lectins in midguts of different species and strains of Glossina. Medical and Veterinary Entomology 3, 7782.
Young, C.J., Turner, D.P., Killick-Kendrick, R., Rioux, J.A. & Leaney, A.J. (1980) Fructose in wild-caught Phlebotomus ariasi and the possible relevance of sugars taken by sandflies to the transmission of leishmaniasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 74, 363366.
Yuval, B. & Schlein, Y. (1986) Leishmaniasis in the Jordan Valley. III. Nocturnal activity of Phlebotomus papatasi (Diptera: Psychodidae) in relation to nutrition and ovarian development. Journal of Medical Entomology 23, 411415.

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Honeydew sugars in wild-caught Italian phlebotomine sandflies (Diptera: Psychodidae) as detected by high performance liquid chromatography

  • Jane A.K. MacVicker (a1), J.S. Moore (a1), D.H. Molyneux (a1) and M. Maroli (a2)

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