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Genetic Diversity and Differentiation among Laboratory and Field Populations of the Soybean Aphid, Aphis glycines

Published online by Cambridge University Press:  27 May 2010

A.P. Michel ,
W. Zhang  and
M.A. Rouf Mian
A.P. Michel*
Affiliation:
Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH44691, USA
W. Zhang
Affiliation:
Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH44691, USA
M.A. Rouf Mian
Affiliation:
USDA-ARS and Department of Horticulture and Crop Sciences, The Ohio State University, Wooster, OH44691, USA
*
*Author for correspondence Fax: +01 330-263-3686 E-mail: michel.70@osu.edu
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Abstract

The soybean aphid, Aphis glycines Matsumura, is a recent invasive pest of soybean in North America. Currently, much research is focused on developing and characterizing soybean cultivars expressing host-plant resistance. During the initial phases of host-plant resistance screening, many of these studies use soybean aphid laboratory populations. Previous studies in other systems have documented substantial differences among laboratory and field populations. Whether or not this pattern exists in A. glycines is unknown, but it is extremely important when estimating the level of selection and virulence to host-plant resistant soybeans. In this study, we used seven microsatellite markers to estimate and compare genetic diversity and differentiation among five laboratory and 12 field populations. Our results indicate that soybean aphid laboratory populations are severely lacking in genotypic diversity and show extreme genetic differentiation among each other and to field populations. Continued use of laboratory populations for initial soybean aphid resistance screening could lead to erroneous estimations of the potential success for host-plant resistance.

Keywords

soybean aphidAphis glycinesmicrosatellitesgenetic diversity
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 100 , Issue 6 , December 2010 , pp. 727 - 734
Copyright
Copyright © Cambridge University Press 2010

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