Skip to main content Accessibility help
×
×
Home

Gene cloning and difference analysis of vitellogenin in Neoseiulus barkeri (Hughes)

  • L. Ding (a1), F. Chen (a2), R. Luo (a1), Q. Pan (a1), C. Wang (a1), S. Yu (a1), L. Cong (a1), H. Liu (a1), H. Li (a1) and C. Ran (a1)...

Abstract

Neoseiulus barkeri (HUGHES) is the natural enemy of spider mites, whiteflies and thrips. Screening for chemically-resistant predatory mites is a practical way to balance the contradiction between the pesticide using and biological control. In this study, the number of eggs laid by fenpropathrin-susceptible and resistant strains of N. barkeri was compared. Additionally, we cloned three N. barkeri vitellogenin (Vg) genes and used quantitative real-time polymerase chain reaction to quantify Vg expression in susceptible and resistant strains. The total number of eggs significantly increased in the fenpropathrin-resistant strain. The full-length cDNA cloning of three N. barkeri Vg genes (NbVg1, NbVg2 and NbVg3) revealed that the open reading frames of NbVg1, NbVg2 and NbVg3 were 5571, 5532 and 4728 bp, encoding 1856, 1843 and 1575 amino acids, respectively. The three N. barkeri Vg possessed the Vitellogenin-N domain (or lipoprotein N-terminal domain (LPD_N)), von Willebrand factor type D domain (VWD) and the domain with unknown function 1943 (DUF1943). The NbVg1 and NbVg2 expression levels were significantly higher in the resistant strain than in the susceptible strain, while the NbVg3 expression level was lower in the resistant strain. Thus, we speculate that the increased number of eggs laid by the fenpropathrin-resistant strain of N. barkeri may be a consequence of changes in Vg gene expression.

Copyright

Corresponding author

*Author for correspondence: Tel: +86-23-6834-9798 Fax: 023-68349005 E-mail: ranchun@cric.cn

Footnotes

Hide All

These authors contributed equally to this study.

Footnotes

References

Hide All
Agnese, M., Verderame, M., Meo, E.D., Prisco, M., Rosati, L., Limatola, E., Gaudio, R.D., Aceto, S. & Andreuccetti, P. (2013) A network system for vitellogenin synthesis in the mussel Mytilus galloprovincialis (L.). Journal of Cellular Physiology 228, 547555.
Arnaud, L. & Haubruge, E. (2002) Insecticide resistance enhances male reproductive success in a beetle. Evolution 56, 24352444.
Auger, P., Bonafos, R., Kreiter, S. & Delorme, R. (2005) A genetic analysis of mancozeb resistance in Typhlodromus pyri (Acari: Phytoseiidae). Experimental and Applied Acarology 3, 8391.
Banks, C.J. & Needham, P.H. (1970) Comparison of the biology of myzus persi-cae sulz. resistant and susceptible to dimethoate. Annals of Applied Biology 66, 465468.
Bjrnson, S. (2008) Natural enemies of mass-reared predatory mites (family phytoseiidae) used for biological pest control. Experimental and Applied Acarology 46, 299306.
Blumenthal, T., Squire, M., Kirtland, S., Cane, J., Donegan, M., Spieth, J. & Sharrock, W. (1984) Cloning of a yolk protein gene family from Caenorhabditis elegans . Journal of Molecular Biology 174, 118.
Boldbaatar, D., Umemiyashirafuji, R., Liao, M., Tanaka, T., Xuan, X.N. & Fujisaki, K. (2010) Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development. Journal of Insect Physiology 56, 15871598.
Bonafos, R., Serrano, E., Auger, P. & Kreiter, S. (2007) Resistance to deltamethrin, lambda-cyhalothrin and chlorpyriphos-ethyl in some populations of Typhlodromus pyri scheuten and Amblyseius andersoni (Chant) (Acari: Phytoseiidae) from vineyards in the south-west of France. Crop Protection 26, 169172.
El-Khatib, Z.I. & Georghiou, G.P. (1985) Comparative fitness of temephos resistant,-susceptible, and hybrid phenotypes of the southern house mosquito (Diptera: Culicidae). Journal of Economic Entomology 78, 10231029.
Fernando, L.C.P., Aratchige, N.S., Kumari, S.L.M.L., Ap puhamy, P.A.L.D. & Hapuarachchi, D.C.L. (2010) Development of a method for mass rearing of Neoseiulus baraki, a mite predatory on the coconut mite, Aceria guerreronis . Cocos 16, 2236.
Ferrari, J.A. & Georghiou, G.P. (1981) Effects on insecticidal selection and treatment on reproductive potential of resistant, susceptible, and heterozygous strains of the southern house mosquito. Journal of Economic Entomology 74, 323327.
Fournier, D., Pralavorio, M., Berge, J.B. & Cuany, A. (1985) Pesticide resistance in Phytoseiidae. pp. 423432 in Helle, W. & Sabelis, M.W. (Eds) Spider Mites: Their Biology, Natural Enemies and Control. Amsterdam, Elsevier, Chapter 3. 5.
Furtado, I.P., Toledo, S., de Moraes, G.J., Kreiter, S. & Knapp, M. (2007) Search for effective natural enemies of Tetranychus evansi (Acari: Tetranychidae) in northwest Argentina. Experimental and Applied Acarology 4, 121127.
Grafton-Cardwell, E.E., Ouyang, Y. & Striggow, R.A. (1999) Predacious mites for control of citrus thrips, Scirtothrips citri (Thysanoptera: Thripidae) in nursery citrus. Biological Control 14, 2936.
Haubruge, E. & Arnaud, L. (2001) Fitness consequences of malathion specific resistance in red flour beetle (Coleoptera: Tenebrionidae) and selection for resistance in the absence of malathion. Journal of Economic Entomology 94, 552557.
Hayward, A., Takahashi, T., Bendena, W. G., Tobe, S. S. & Hui, J. H. (2010) Comparative genomic and phylogenetic analysis of vitellogenin and other large lipid transfer proteins in metazoans. FEBS Letters . 584, 12731278.
Hessein, N.A. & Parrella, M.P. (1991) Predatory mites help control thrips on floriculture crops. California Agriculture 44, 1921.
Jafari, S., Fathipour, Y. & Faraji, F. (2012) Temperature-dependent development of Neoseiulus barkeri (Acari: Phytoseiidae) on Tetranychus urticae (acari: Tetranychidae) at seven constant temperatures. Insect Science 19, 220228.
Khalil, S.M., Donohue, K.V., Thompson, D.M., Jeffers, L.A., Ananthapadmanaban, U., Sonenshine, D.E., Mitchell, R.D. & Roe, R.M. (2011). Full-length sequence, regulation and developmental studies of a second vitellogenin gene from the American dog tick, Dermacentor variabilis . Journal of Insect Physiology 57, 400408.
Lee, C.Y., Yap, H.H., Chong, N.L. & Lee, R.S.T. (1996) Insecticide resistance and synergism in field collected German cockroaches (Dictyoptera: Blattellidae) in Peninsular Malaysia. Bulletin of Entomological Research 86, 675682.
Lin, C., Chen, F., Yu, S., Ding, L., Yang, J., Luo, R., Tian, H., Li, H., Liu, H. & Ran, C. (2016) Transcriptome and difference analysis of fenpropathrin resistant predatory mite, Neoseiulus barkeri (Hughes). International Journal of Molecular Sciences 17, 704.
Liu, C., Mao, J. & Zeng, F. (2015) Chrysopa septempunctata (Neuroptera: Chrysopidae) vitellogenin functions through effects on egg production and hatching. Journal of Economic Entomology 108, 27792788.
Liu, X., Shen, G., Xu, H. & He, L. (2016) The fenpropathrin resistant Tetranychus cinnabarinus showed increased fecundity with high content of vitellogenin and vitellogenin receptor. Pesticide Biochemistry and Physiology 134, 3138.
Lu, K., Shu, Y., Zhou, J., Zhang, X., Zhang, X., Chen, M., Yao, Q., Zhou, Q. & Zhang, W. (2015) Molecular characterization and RNA interference analysis of vitellogenin receptor from Nilaparvata lugens (Stal). Journal of Insect Physiology 73, 2029.
Matsubara, T., Ohkubo, N., Andoh, T., Sullivan, C.V. & Hara, A. (1999) Two forms of vitellogenin, yielding two distinct lipovitellins, play different roles during oocyte maturation and early development of barfin flounder, Verasper moseri, a marine teleost that spawns pelagic eggs. Developmental Biology 213, 1832.
Melo, A.C., Valle, D., Machado, E.A., Salerno, A.P., Paiva-Silva, G.O., Cunha, E.S.N.L., de Souza, W. & Masuda, H. (2000) Synthesis of vitellogenin by thefollicle cells of Rhodnius prolixus . Insect Biochemistry and Molecular Biology 30, 549557.
Miyo, T. & Oguma, Y. (2002) Negative correlations between resistance to three organophosphate insecticides and productivity within a natural population of Drosophila melanogaster (Diptera: Drosophilidae). Journal of Economic Entomology 95, 12291238.
Momen, F.M. (1995) Feeding, development and reproduction of Amblyseiu barkeri (Acarina: Phytoseiidae) on various kinds of food substances. Acarologia 36, 101105.
Ni, J., Zeng, Z., Kong, D., Hou, L., Huang, H. & Ke, C. (2014) Vitellogenin of Fujian oyster, Crassostrea angulata: synthesized in the ovary and controlled by estradiol-17. General and Comparative Endocrinology 20, 3543.
Nicastro, R.L., Sato, M.E. & da Silva, M.Z. (2011) Fitness costs associated with milbemectin resistance in the two-spotted spider mite Tetranychus urticae . International Journal of Pest Management 57, 223228.
Nomikou, M., Janssen, A., Schraag, R. & Sabelis, M.W. (2001) Phytoseiid predators as potential biological control agents for Bemisia tabaci . Experimental and Applied Acarology 25, 271291.
Pathan, A.K., Sayyed, A.H., Aslam, M., Liu, T.X., Razzaq, M. & Gillani, W.A. (2010) Resistance to pyrethroids and organophosphates increased fitness and predation potential of Chrysoperla carnae (Neuroptera: Chrysopidae). Journal of Economic Entomology 103, 823834.
Qiu, J., He, Y., Zhang, J., Kang, K., Li, T. & Zhang, W. (2016) Discovery and functional identification of fecundity-related genes in the brown planthopper by large-scale RNA interference. Insect Molecular Biology 25, 724733.
Raikhel, A.S. & Dhadialla, T.S. (1992) Accumulation of yolk proteins in insect oocytes. Entomology 37, 217251.
Richardson, M. (1998) Pesticides – friend or foe? Water Science and Technology 37, 1925.
Ross, M.H. (1991) A comparison of reproduction and longevity in pyrethroidresistant and susceptible German cockroach (Blattodea: Blattellidae) field-collected strains. Journal of Entomological Science 26, 408418.
Salman, S.Y. & Ay, R. (2013) Analysis of hexythiazox resistance mechanisms in a laboratory selected predatory mite Neoseiulus californicus (Acari: Phytoseiidae). Turkish Journal of Entomology 3, 409422.
Schip, F.D.V.H., Samallo, J., Broos, J., Ophuis, J., Mojet, M., Gruber, M. & Geert, A.B. (1987) Nucleotide sequence of a chicken vitellogenin gene and derived amino acid sequence of the encoded yolk precursor protein. Journal of Molecular Biology 196, 245260.
Shu, Y., Zhou, J., Tang, W., Lu, K., Zhou, Q. & Zhang, G. (2009) Molecular characterization and expression pattern of Spodoptera litura (Lepidoptera: Noctuidae) vitellogenin, and its response to lead stress. Journal of Insect Physiology 55, 608616.
Shu, Y.H., Wang, J.W., Lu, K., Zhou, J.L., Zhou, Q. & Zhang, G.R. (2011) The first vitellogenin receptor from a lepidopteran insect: molecular characterization, expression patterns and RNA interference analysis. Insect Molecular Biology 20, 6173.
Silva, R. & Fischer, A.H. (1989) The major and minor chicken vitellogenin genes are each adjacent to partially deleted pseudogene copies of the other. Molecular and Cellular Biology 9, 35573562.
Smolenaars, M. M., Madsen, O., Rodenburg, K. W. & Dj, V. D. H. (2007) Molecular diversity and evolution of the large lipid transfer protein superfamily. Journal of Lipid Research 48, 489502.
Stocco, R.S., Sato, M.E. & Santos, T.L. (2016) Stability and fitness costs associated with etoxazole resistance in Tetranychus urticae (Acari: Tetranychidae). Experimental and Applied Acarology 69, 113.
Tran, T.K.A., Macfarlane, G.R., Kong, R.Y.C., OConnor, W.A. & Yu, R.M.K. (2016) Mechanistic insights into induction of vitellogenin gene expression by estrogens in Sydney rock oysters, Saccostrea glomerata . Aquatic Toxicology 174, 146158.
Trapp, J., Armengaud, J., Gaillard, J.C., Pible, O., Chaumot, A. & Geffard, O. (2016) High-throughput proteome dynamics for discovery of key proteins in sentinel species: unsuspected vitellogenins diversity in the crustacean Gammarus fossarum . Journal of Proteomics 146, 207214.
Tufail, M. & Takeda, M. (2005) Molecular cloning, characterization and regulation of the cockroach vitellogenin receptor during oogenesis. Insect Molecular Biology 14, 389401.
Tufail, M. & Takeda, M. (2008) Molecular characteristics of insect vitellogenins. Journal of Insect Physiology 54, 14471458.
Tuovinen, T. & Lindqvist, I. (2010) Maintenance of predatory phytoseiid mites for preventive control of strawberry tarsonemid mite phytonemus pallidus in strawberry plant propagation. Biological Control 54, 119125.
Veerana, M., Kubera, A. & Ngernsiri, L. (2014) Analysis of the vitellogenin gene of rice moth, Corcyra cephalonica Stainton. Archives of Insect Biochemistry and Physiology, 87, 126147.
Wahli, W., Dawid, I.B., Wyler, T., Jaggi, R.B., Weber, R. & Gu, R. (1979). Vitellogenin in Xenopus laevis is encoded in a small family of genes. Cell 1, 535549.
Yao, H., Zheng, W., Tariq, K. & Zhang, H. (2014) Functional and numerical responses of three species of predatory Phytoseiid mites (Acari: Phytoseiidae) to Thrips flavidulus (Thysanoptera: Thripidae). Neotropical Entomology 43, 437445.
Zhai, Y., Sun, Z., Zhang, J., Kang, K., Chen, J. & Zhang, W. (2015) Activation of the tor signalling pathway by glutamine regulates insect fecundity. Scientic Reports, 5, 10694.
Zhang, S., Wang, S., Li, H. & Li, L. (2010) Vitellogenin, a multivalent sensor and an antimicrobial effector. International Journal of Biochemistry & Cell Biology 43, 303305.
Zhang, W.N., Xiao, H.J., Liang, G. M., Guo, Y.Y. & Wu, K.M. (2014) Tradeoff between reproduction and resistance evolution to bt-toxin in Helicoverpa armigera: regulated by vitellogenin gene expression. Bulletin of Entomological Research 104, 444452.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Bulletin of Entomological Research
  • ISSN: 0007-4853
  • EISSN: 1475-2670
  • URL: /core/journals/bulletin-of-entomological-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed