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The formulation of the essential oil of Piper aduncum Linnaeus (Piperales: Piperaceae) increases its efficacy as an insect repellent

  • S.N.H. Mamood (a1), O. Hidayatulfathi (a1), S.B. Budin (a1), G. Ahmad Rohi (a1) and M.H. Zulfakar (a2)...

Abstract

The essential oil (EO) of Piper aduncum Linnaeus, known as ‘sireh lada’ to locals Malaysian, has the potential to be used as an alternative to synthetic insect repellents such as N,N-diethyl-meta-toluamide. However, the EO's efficacy as a repellent decreases after application due to the high volatility of its active ingredients. A number of studies have showed that optimizing the formulation of plant-based EOs can improve their efficacy as repellents. The present study sought to evaluate the effectiveness of 10% P. aduncum EO in ethanol and in three different semisolid formulations: ointment, cream and gel. These formulations were tested on Aedes aegypti under laboratory conditions. Each formulation was applied to the subject's hands, which were then inserted into a cage containing 25 nulliparous A. aegypti. The number of mosquitoes landing on or biting each subject's hand was recorded, and the repellency percentage, landing/biting percentage and protection time for each of the formulations were compared. There were no statistically significant differences between the semisolid EO formulations with regards to the repellency percentage and the landing/biting percentage at 4 h post-application. All three semisolid EO formulations were able to repel >65% of the A. aegypti at 4 h post-application. The EO ointment formulation provided a protection time (182.5 ± 16.01 min) that was statistically significantly longer than that associated with the EO gel formulation (97.5 ± 14.93 min). Meanwhile, the EO cream formulation provided a protection time of 162.5 ± 6.29 min. As the EO cream and ointment formulations displayed better repellent properties than the EO gel formulation, they appear to be the most promising P. aduncum EO formulations to be developed and commercialized as alternatives to synthetic repellents.

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*Author for correspondence Phone: 6012-3736549/603-92897693 Fax: +60326929032 E-mail: hida_othman@yahoo.co.uk

References

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Aisien, M.S.O., Imasuen, A.A., Wagbatsomaand, V.A. & Ayinde, B.A. (2004) Preliminary evaluation of the repellent activity of some essential oils against Simulium damnosum s.l., the vector of human onchocerciasis. International Journal of Tropical Insect Science 24(2), 196199.
Amer, A. & Mehlhorn, H. (2006) Repellency effect of forty-one essential oils against Aedes, Anopheles, and Culex mosquitoes. Parasitology Research 99(4), 478490.
Barnard, D.R. (2000) Global Collaboration for Development of Pesticides for Public Health: Repellents and Toxicants for Personal Protection: a WHO Position Paper. Geneva, World Health Organization.
Bissinger, B.W., Schmidt, J.P., Owens, J.J., Mitchell, S.M. & Kennedy, M.K. (2014) Performance of the plant-based repellent TT-4302 against mosquitoes in the laboratory and field and comparative efficacy to 16 mosquito repellents against Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology 51(2), 392399.
Blackwell, A., Stuart, A.E. & Estambale, B.A. (2003) The repellent and antifeedant activity of Myrica gale oil against Aedes aegypti mosquitoes and its enhancement by the addition of salicyluric acid. Journal of the Royal College of Physicians of Edinburgh 33, 209214.
Browne, L.B. (1997) Host-related responses and their suppression: some behavioral considerations. pp. 117127 in Shorey, H.H. & McKelvey, J.J. Jr. (Eds) Chemical Control of Insect Behavior. New York, Wiley.
Burger, W.C. (1971) Piperaceae. Fieldiana (Botany) 35, 7996.
Choochote, W., Chaithong, U., Kamsuk, K., Jitpakdi, A., Tippawangkosol, P., Tuetun, B., Champakaew, D. & Pitasawat, B. (2007) Repellent activity of selected essential oils against Aedes aegypti . Fitoterapia 78(5), 359364.
Chou, J.T., Rossignol, P.A. & Ayres, J.W. (1997) Evaluation of commercial insect repellents on human skin against Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology 34(6), 624630.
Ciccio, J.F. & Ballestero, C.M. (1997) Constituyentes volátiles de las hojas y espigas de Piper aduncum (Piperaceae) de Costa Rica. Revista de Biologia Tropical 45, 783790.
Das, M.K. & Ansari, M.A. (2003) Evaluation of repellent action of Cymbopogan martinii Stapf var sofia oil against Anopheles sundaicus in tribal villages of Car Nicobar Island, Andaman & Nicobar Islands, India. Journal of Vector Borne Diseases 40(3–4), 101104.
Florence, A.T. & Attwood, D. (1990) Physicochemical Principles of Pharmacy. 2nd edn. Basingstoke, Macmillan Press.
Fradin, M.S. & Day, J.F. (2002) Comparative efficacy of insect repellents against mosquito bites. New England Journal of Medicine 347(1), 1318.
Frances, S.P. & Wirtz, R.A. (2005) Repellents: past, present, and future. Journal of the American Mosquito Control Association 21, 13.
Gillij, Y.G., Gleiser, R.M. & Zygadlo, J.A. (2008) Mosquito repellent activity of essential oils of aromatic plants growing in Argentina. Bioresource Technology 99(7), 25072515.
Golenda, C.F., Solberg, V.B., Burge, R., Gambel, J.M. & Wirtz, R.A. (1999) Gender-related efficacy difference to an extended duration formulation of topical N,N-diethyl-M-toluamide (DEET). American Journal of Tropical Medicine and Hygiene 60(4), 654657.
Goodyer, L. & Song, J. (2014) Mosquito bite-avoidance attitudes and behaviors in travelers at risk of malaria. Journal of Travel Medicine 21(1), 3338.
Govere, J., Durrheim, D.N., Baker, L., Hunt, R. & Coetzee, M. (2000) Efficacy of three insect repellents against the malaria vector Anopheles arabiensis . Medical and Veterinary Entomology 14(4), 441444.
Gupta, R.K. & Rutledge, L.C. (1991) Controlled release repellent formulations on human volunteers under three climatic regimens. Journal of the American Mosquito Control Association 7(3), 490493.
Gupta, R.K. & Rutledge, L.C. (1994) Role of repellents in vector control and disease prevention. American Journal of Tropical Medicine and Hygiene 50(6 Suppl), 8286.
Health Protection Agency (2007) Guidelines for Malaria Prevention in Travelers from the United Kingdom. London, Health Protection Agency.
Hidayatulfathi, O., Sallehuddin, S. & Ibrahim, J. (2004) Adulticidal activity of some Malaysian plant extracts against Aedes aegypti Linnaeus. Tropical Biomedicine 21(2), 6167.
Katz, T.M., Miller, J.H. & Hebert, A.A. (2008) Insect repellents: historical perspectives and new developments. Journal of the American Academy of Dermatology 58(5), 865871.
Keziah, E.A., Nukenine, E.N., Danga, S.P., Younoussa, L. & Esimone, C.O. (2015) Creams formulated with Ocimum gratissimum L. and Lantana camara L. crude extracts and fractions as mosquito repellents against Aedes aegypti L. (Diptera: Culicidae). Journal of Insect Science 15, 45.
Logan, J.G., Stanczyk, N.M., Hassanali, A., Kemei, J., Santana, A.E.G., Ribeiro, K.A.L., Pickett, J.A. & Mordue Luntz, A.J. (2010) Arm-in-cage testing of natural human-derived mosquito repellents. Malaria Journal 9, 239.
Meepagala, K.M., Bernier, U.R., Burandt, C. & Duke, S.O. (2013) Mosquito repellents based on a natural chromene analogue with longer duration of action than N,N-diethyl-meta-toluamide (DEET). Journal of Agricultural and Food Chemistry 61(39), 92939297.
Meltzer, E. & Schwartz, E. (2009) A travel medicine view of dengue and dengue hemorrhagic fever. Travel Medicine and Infectious Disease 7(5), 278283.
Misni, N., Sulaiman, S. & Othman, H. (2008) The repellent activity of Piper aduncum Linn (Family: Piperaceae) essential oil against Aedes aegypti using human volunteers. Journal of Tropical Medicine & Parasitology 31, 6369.
Mukesh, Y., Savitri, P., Kaushik, R. & Singh, N.P. (2014) Studies on repellent activity of seed oils alone and in combination on mosquito, Aedes aegypti . Journal of Environmental Biology 35(5), 917922.
Nerio, L.S., Olivero-Verbel, J. & Stashenko, E. (2010) Repellent activity of essential oils: a review. Bioresource Technology 101(1), 372378.
Novak, R.J. & Gerberg, E.J. (2005) Natural-based repellent products: efficacy for military and general public uses. Journal of the American Mosquito Control Association 21, 711.
Olander, L.P., Scatena, F.N. & Silver, W.L. (1998) Impacts of disturbance initiated by road construction in a subtropical cloud forest in the Luquillo Experimental Forest, Puerto Rico. Forest Ecology and Management 109, 3349.
Omotosho, J.A., Whateley, T.L., Law, T.K. & Florence, A.T. (1986) The nature of the oil phase and the release of solutes from multiple (w/o/w) emulsions. Journal of Pharmacy and Pharmacology 38(12), 865870.
Osimitz, T.G. & Grothaus, R.H. (1995) The present safety assessment of DEET. Journal of the American Mosquito Control Association 11(2), 274278.
Oyedele, A.O., Orafidiya, L.O., Lamikanra, A. & Olaifa, J.I. (2000) Volatility and mosquito repellency of Hemizygia welwitschii Rolfe oil and its formulations. International Journal of Tropical Insect Science 20(2), 123128.
Oyedele, A.O., Gbolade, A.A., Sosan, M.B., Adewoyin, F.B., Soyelu, O.L. & Orafidiya, O.O. (2002) Formulation of an effective mosquito-repellent topical product from lemongrass oil. Phytomedicine 9(3), 259262.
Phasomkusolsil, S. & Soonwera, M. (2010) Insect repellent activity of medicinal plant oils against Aedes aegypti (Linn.), Anopheles minimus (Theobald) and Culex quinquefasciatus Say based on protection time and biting rate. Southeast Asian Journal of Tropical Medicine and Public Health 41(4), 831840.
Pohlit, A.M., Pinto, A.C.S. & Mause, R. (2006) Piper aduncum L.: pluripotente plant and important phytochemical substance source. Revista Fitos 2, 718.
Rozendaal, J.A. (1997) Vector Control: Methods for use by Individuals and Communities. Geneva, World Health Organization.
Schwartz, E., Weld, L.H., Wilder-Smith, A., Von Sonnenburg, F., Keystone, J.S., Kain, K.C., Torresi, J. & Freedman, D.O. (2008) Seasonality, annual trends, and characteristics of dengue among ill returned travelers, 1997–2006. Emerging Infectious Diseases 14(7), 10811088.
Sritabutra, D., Soonwera, M., Waltanachanobon, S. & Poungjai, S. (2011) Evaluation of herbal essential oil as repellents against Aedes aegypti (L.) and Anopheles dirus Peyton & Harrion. Asian Pacific Journal of Tropical Biomedicine 1(1), 124128.
Standard and Industrial Research Institute of Malaysia (2007) Household Insecticide Products-Personal Mosquito Repellent – Evaluation Method for Biological Efficacy (MS1497:2007), Malaysia. Available online at http://www.freestd.us/soft4/2003367.htm.
Stienlauf, S., Segal, G., Sidi, Y. & Schwartz, E. (2005) Epidemiology of travel-related hospitalization. Journal of Travel Medicine 12(3), 136141.
Thavara, U., Tawatsin, A. & Chompoosri, J. (2002) Phytochemicals as repellents against mosquitoes in Thailand. pp. 244–250 in Proceedings of International Conference on Biopesticide 3, 21–26 April 2002, Kuala Lumpur.
Vatandoost, H. & Hanafi-Bojd, A.A. (2008) Laboratory evaluation of 3 repellents against Anopheles stephensi in the Islamic Republic of Iran. Eastern Mediterranean Health Journal 14(2), 260267.
Zhu, B.C.R., Henderson, G., Chen, F., Fei, H. & Laine, R.A. (2001) Evaluation of vetiver oil and seven insect-active essential oils against the Formosan subterranean termite. Journal of Chemical Ecology 27(8), 16171625.

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