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Exploring miRNA-mRNA regulatory modules responding to tannic acid stress in Micromelalopha troglodyta (Graeser) (Lepidoptera: Notodontidae) via small RNA sequencing

Published online by Cambridge University Press:  12 July 2022

Zhiqiang Wang ,
Fang Tang Open the ORCID record for Fang Tang [Opens in a new window] ,
Meng Xu  and
Tengfei Shen
Zhiqiang Wang
Affiliation:
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, People's Republic of China College of Forestry, Nanjing Forestry University, Nanjing 210037, People's Republic of China
Fang Tang*
Affiliation:
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, People's Republic of China College of Forestry, Nanjing Forestry University, Nanjing 210037, People's Republic of China
Meng Xu
Affiliation:
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, People's Republic of China College of Forestry, Nanjing Forestry University, Nanjing 210037, People's Republic of China
Tengfei Shen
Affiliation:
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, People's Republic of China College of Forestry, Nanjing Forestry University, Nanjing 210037, People's Republic of China
*
Author for correspondence: Fang Tang, Email: tangfang76@foxmail.com
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Abstract

MicroRNAs (miRNAs) are small noncoding RNAs (sRNAs) that regulate gene expression by inhibiting translation or degrading mRNA. Although the functions of miRNAs in many biological processes have been reported, there is currently no research on the possible roles of miRNAs in Micromelalopha troglodyta (Graeser) involved in the response of plant allelochemicals. In this article, six sRNA libraries (three treated with tanic acid and three control) from M. troglodyta were constructed using Illumina sequencing. From the results, 312 known and 43 novel miRNAs were differentially expressed. Notably, some of the most abundant miRNAs, such as miR-432, miR-541-3p, and miR-4448, involved in important physiological processes were also identified. To better understand the function of the targeted genes, we performed Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The results indicated that differentially expressed miRNA targets were involved in metabolism, development, hormone biosynthesis, and immunity. Finally, we visualized a miRNA-mRNA regulatory module that supports the role of miRNAs in host–allelochemical interactions. To our knowledge, this is the first report on miRNAs responding to tannic acid in M. troglodyta. This study provides indispensable information for understanding the potential roles of miRNAs in M. troglodyta and the applications of these miRNAs in M. troglodyta management.

Keywords

Host–allelochemical interactionIllumina sequencingmicroRNAstannic acid
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 113 , Issue 1 , February 2023 , pp. 86 - 97
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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