Skip to main content Accessibility help

DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae)

  • Y.J. Wang (a1), Z.H. Li (a1), S.F. Zhang (a2), Z. Varadínová (a3) (a4), F. Jiang (a1), Z. Kučerová (a4), V. Stejskal (a4), G. Opit (a5), Y. Cao (a6) and F.J. Li (a6)...


Several species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schönherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658 bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptolestes species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database.


Corresponding author

* Author for correspondence Phone: +86 -10- 62731299 Fax: +86 -10- 62733404 E-mail:


Hide All
Antonini, G., Coletti, G., Serrani, L., Tronci, C., Cristofaro, M. & Smith, L. (2009) Using molecular genetics to identify immature specimens of the weevil Ceratapion basicorne (Coleoptera: Apionidae). Biological Control 51, 152157.
Arbogast, R.T. (1991) Identification of Cryptolestes ferrugineus and Cryptolestes pusillus (Coleoptera: Cucujidae): a practical character for sorting large samples by species. Entomological News 102, 3336.
Armstrong, K.F. & Ball, S.L. (2005) DNA barcoding for biosecurity: invasive species identification. Philosophical Transactions of the Royal Society B 360, 18131823.
Arnaud, L., Brostaux, Y., Assié, L.K., Gaspar, C. & Haubruge, E. (2002) Increased fecundity of malathion-specific resistant beetles in absence of insecticide pressure. Heredity 89, 425429.
Banks, H.J. (1979) Identification of stored product Cryptolestes spp. (Coleoptera: Cucujidae): a rapid technique for preparation of suitable mounts. Australian Journal of Entomology 18, 217222.
Barr, N.B., Islam, M.S., De Meyer, M. & McPheron, B.A. (2012) Molecular identification of Ceratitis capitata (Diptera: Tephritidae) using DNA sequences of the COI barcode region. Annals of the Entomological Society of America 105, 339350.
Beltrà, A., Soto, A. & Malausa, T. (2012) Molecular and morphological characterisation of Pseudococcidae surveyed on crops and ornamental plants in Spain. Bulletin of Entomological Research 102, 165172.
Caterino, M.S. & Tishechkin, A.K. (2006) DNA identification and morphological description of the first confirmed larvae of Hetaeriinae (Coleoptera: Histeridae). Systematic Entomology 31, 405418.
Clement, M., Posada, D. & Crandall, K.A. (2000) TCA: a computer program to estimate gene genealogies. Molecular Ecology 9, 16571659.
Darling, J.A. & Blum, M.J. (2007) DNA-based methods for monitoring invasive species: a review and prospectus. Biological Invasions 9, 751765.
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9, 772.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294299.
Foottit, R.G., Maw, H.E.L., Dohlen, C.D.V. & Hebert, P.D.N. (2008) Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes. Molecular Ecology Resources 8, 11891201.
Freeman, J.A. (1952) Laemophloeus spp. as a major pest of stored grain. Plant Pathology 1, 6976.
Freeman, J.A. (1967) Problems of infestation of commodities carried by sea with special references to imports into Great Britain. EPPO Publication, Series A, No 46E, Lisbon, Stored Product Conference.
Frézal, L. & Leblois, R. (2008) Four years of DNA barcoding: current advances and prospects. Infection Genetics and Evolution 8, 727736.
Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59, 307321.
Hagstrum, D. & Subramanyam, B. (2009) Stored-product Insect Resource. St. Paul, MN, AACC Press, p. 509.
Hajibabaei, M., Singer, G.A.C., Hebert, P.D.N. & Hickey, D.A. (2007) DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. Trends in Genetics 23, 167172.
Halstead, D.G.H. (1993) Keys for the identification of beetles associated with stored products-II. Laemophloeidae, Passandridae and Silvanidae. Journal of Stored Products Research 29, 99197.
Hart, M.W. & Sunday, J. (2007) Things fall apart: biological species from unconnected parsimony networks. Biology Letters 3, 509512.
Hebert, P.D.N., Cywinska, A., Ball, S.L. & DeWaard, J.R. (2003 a) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B 270, 313321.
Hebert, P.D.N., Ratnasingham, S. & deWaard, J.R. (2003 b) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London B 270 (Suppl 1), S96S99.
Hebert, P.D.N., Stoeckle, M.Y., Zemlak, T.S. & Francis, C.M. (2004) Identification of birds through DNA barcodes. PLoS Biology 2, 16571663.
Hebert, P.D.N., DeWaard, J.R. & Landry, J.F. (2010) DNA barcodes for 1/1000 of the animal kingdom. Biology Letters 6, 359362.
Hossain, M., Verner, P.H. & Rezaur, R. (1986) Taxonomic descriptions of the mature larvae of six species of Cryptolestes (Coleoptera: Cucujidae). Bangladesh Journal of Zoology 14, 139148.
Howe, R.W., & Lefkovitch, L.P. (1957) The distribution of the storage species of Cryptolestes (Col.: Cucujidae). Bulletin of Entomological Research 48, 795809.
Kučerová, Z. & Stejskal, V. (2002) Comparative egg morphology of Silvanid and Laemophloeid beetles (Coleoptera) occurring in stored products. Journal of Stored Products Research 38, 219227.
Lefébure, T., Douady, C.J., Gouy, M. & Gibert, J. (2006) Relationship between morphological taxonomy and molecular divergence within Crustacea: proposal of a molecular threshold to help species delimitation. Molecular Phylogenetics and Evolution 40, 435447.
Lefkovitch, L.P. (1958) Unusual antenna1 characters in some Laemophloeinae (Coleoptera: Cucujidae) and their taxonomic importance. Proceedings of the Royal Entomological Society of London (B) 27, 93100.
Lefkovitch, L.P. (1962) A revision of African Laemophloeinae (Coleoptera: Cucujidae). Bulletin of the British Museum of Natural History (Entomology) 12, 167245.
Lefkovitch, L.P. & Currie, J.E. (1967) Some morphological, biological and genetical differences between Cryptolestes pusillus fuscus ssp.n. and C. pusillus pusillus (Schönherr) (Coleoptera, Cucujidae). Journal of Stored Products Research 3, 311320.
Librado, P. & Rozas, J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 14511452.
Löbl, I. & Smetana, A. (Eds) (2007) Catalogue of Palaearctic Coleoptera. Volume 4: Elateroidea – Derodontoidea – Bostrichoidea – Lymexiloidea – Cleroidea – Cucujoidea. Stenstrup, Apollo Books, p. 935.
Meiklejohn, K.A., Wallman, J.F. & Dowton, M. (2011) DNA-based identification of forensically important Australian Sarcophagidae (Diptera). International Journal of Legal Medicine 125, 2732.
Meyer, C.P. & Paulay, G. (2005) DNA barcoding: error rates based on comprehensive sampling. PLoS Biology 3, 22292238.
Pamilo, P., Viljakainen, L. & Vihavainen, A. (2007) Exceptionally high density of NUMTs in the honeybee genome. Molecular Biology and Evolution 24, 13401346.
Puillandre, N., Lambert, A., Brouillet, S. & Achaz, G. (2012) ABGD, automated barcode gap discovery for primary species delimitation. Molecular Ecology 21, 18641877.
Raupach, M.J., Astrin, J.J., Hannig, K., Peters, M.K., Stoeckle, M.Y. & Wägele, J. (2010) Molecular species identification of Central European ground beetles (Coleoptera: Carabidae) using nuclear rDNA expansion segments and DNA barcodes. Frontiers in Zoology 7, 115.
Rees, D. (2004) Insects of Stored Products. Collingwood, Victoria, Australia, CSIRO Publishing, p. 192.
Shoemaker, D.D., Dyer, K.A., Ahrens, M., McAbee, K. & Jaenike, J. (2004) Decreased diversity but increased substitution rate in host mtDNA as a consequence of Wolbachia endosymbiont infection. Genetics 168, 20492058.
Sinha, R.N. (1975) Climate and the infestation of stored cereals by insects. pp. 117141 in Proceedings of the first International Working Conference on Stored Products Entomology. 1974, Savannah, USA.
Song, H., Bhay, J.E., Whiting, M.F. & Crandall, K.A. (2008) Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified. Proceeding of the National Academy of Sciences of the USA 105, 1348613491.
Stejskal, V. & Hubert, J. (2008) Risk of occupational allergy to stored grain arthropods and false pest-risk perception in Czech grain stores. Annals of Agricultural and Environmental Medicine 15, 2935.
Stejskal, V., Hubert, J., Kučerová, Z., Munzbergová, Z., Lukáš, J. & Žďárková, E. (2003) The influence of type of storage on pest infestation of stored grain in the Czech Republic. Plant Soil Environment 49, 5562.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 27312739.
Templeton, A.R., Crandall, K.A. & Sing, C.F. (1992) A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics 132, 619633.
Thomas, M.C. (1988) A revision of the new world species of Cryptolestes Ganglbauer (Coleoptera: Cucujidae: Laemophloeinae). Insecta Mundi 2, 4365.
Throne, J.E., Doehlert, D.C. & McMullen, M.S. (2003) Susceptibility of commercial oat cultivars to Cryptolestes pusillus and Oryzaephilus surinamensis . Journal of Stored Products Research 39, 213223.
Trematerra, P., Stejskal, V. & Hubert, J. (2011) The monitoring of semolina contamination by insect fragments using the light filth method in an Italian mill. Food Control 22, 10211026.
Wiemers, M. & Fiedler, K. (2007) Does the DNA barcoding gap exist? – a case study in blue butterflies (Lepidoptera: Lycaenidae). Frontiers in Zoology 4, 116.
Wong, E.H.K., Shivji, M.S., & Hanner, R.H. (2009) Identifying sharks with DNA barcodes: assessing the utility of a nucleotide diagnostic approach. Molecular Ecology Resources 9, 243256.
Xiao, J.H., Wang, N.X., Li, Y.W., Murphy, R.W., Wan, D.G., Niu, L.M., Hu, H.Y., Fu, Y.G. & Huang, D.W. (2010) Molecular approaches to identify cryptic species and polymorphic species within a complex community of fig wasps. PLoS ONE 5, e15067. doi: 10.1371/journal.pone.0015067.
Yang, Q.Q., Kučerová, Z., Li, Z.H., Kalinović, I., Stejskal, V., Opit, G. & Cao, Y. (2012) Diagnosis of Liposcelis entomophila (Insecta: Psocodea: Liposcelididae) based on morphological characteristics and DNA barcodes. Journal of Stored Products Research 48, 120125.
Yang, Q.Q., Zhao, S., Kučerová, Z., Stejskal, V., Opit, G., Qin, M., Cao, Y., Li, F.J. & Li, Z.H. (2013) Validation of the 16S rDNA and COI DNA Barcoding technique for rapid molecular identification of stored product Psocids (Insecta: Psocodea: Liposcelididae). Journal of Economic Entomology 106, 419425.


DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae)

  • Y.J. Wang (a1), Z.H. Li (a1), S.F. Zhang (a2), Z. Varadínová (a3) (a4), F. Jiang (a1), Z. Kučerová (a4), V. Stejskal (a4), G. Opit (a5), Y. Cao (a6) and F.J. Li (a6)...


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed