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Determination of mitochondrial DNA haplotypes from sting remnants of the honeybee Apis mellifera (Hymenoptera:Apidae)

Published online by Cambridge University Press:  10 July 2009

W.S. Sheppard ,
M.C. Arias  and
H. Shimanuki
W.S. Sheppard*
Affiliation:
USDA-ARS Bee Research Laboratory, Beltsville, USA
M.C. Arias
Affiliation:
USDA-ARS Bee Research Laboratory, Beltsville, USA
H. Shimanuki
Affiliation:
USDA-ARS Bee Research Laboratory, Beltsville, USA
*
W.S. Sheppard, USDA-ARS Bee Research Laboratory Bldg 476, BARC-E, Beltsville, MD 20705, USA.
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Abstract

Africanized honeybees are presently expanding their geographic range within the USA and are considered undesirable due to their substantial defensive behaviour. Africanized honeybees can be differentiated from honeybees of European ancestry using discriminant analysis of morphological characters, based on a minimum of ten intact specimens per colony. In this paper we report a PCR-based method suitable for the identification of African or European mtDNA from sting remnants, such as typically remain on victims following a stinging incident. We experimentally simulated collection and shipment conditions with dried and alcohol preservation of stings, and also report that the method was suitable for sting remnants stored with a victim sample for over one year in alcohol. The determination of mtDNA haplotypes from stinger remnants must be tempered by the constraints inherent in interpretation of mtDNA haplotype data. Such data do not provide any information regarding the genetic contribution of the paternal lineage. However, in geographic areas where baseline information regarding European haplotype frequencies is known, the detection of a different mtDNA haplotype in stingers from a victim, especially when associated with observations of extreme defensive behaviour, would certainly be suggestive of Africanization.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 84 , Issue 4 , December 1994 , pp. 551 - 554
Copyright
Copyright © Cambridge University Press 1994

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References

Bernard, J.H. (1973) Studies of 400 Hymenoptera sting deaths in the United States. Journal of Allergy and Clinical Immunology 52, 259–264.CrossRefGoogle Scholar
Collins, A.M., Rinderer, T.E., Harbo, J.R. & Bolton, A.R. (1982) Colony defense by Africanized and European honeybees. Science 218, 72–74.CrossRefGoogle Scholar
Crozier, Y.C., Koulianos, S. & Crozier, R.H. (1991) An improved test for Africanized honeybee mitochondrial DNA. Experientia 47, 968 –969.CrossRefGoogle ScholarPubMed
Daly, H.V. & Balling, St. (1978) Identification of Africanized honeybees in the western hemisphere by discriminant analysis. Journal of the Kansas Entomological Society 51, 857–869.Google Scholar
Gaensslen, R.E. & Lee, H.C. (1990) Genetic markers in human bone tissue. Forensic Science Review 2, 125–146.Google ScholarPubMed
Hall, G.H. & Muralidharan, K. (1989) Evidence from mitochon drial DNA that African honeybees spread as continuous maternal lineages. Nature 339, 213–215.CrossRefGoogle Scholar
Hall, G.H. & Smith, D.R. (1991) Distinguishing African and European honeybee matrilines using amplified mitochondrial DNA. Proceedings of National Academy of Sciences USA 88,4548–4552.CrossRefGoogle ScholarPubMed
Meixner, M.D., Sheppard, W.S. & Poklukar, J. (1993) Asymmetrical distribution of a mitochondrial DNA polymorphism between two introgressing honey bee subspecies. Apidologie 24, 147–153.CrossRefGoogle Scholar
Novoa, E.G. & Page, R.E. (1993) Backcrossing Africanized honey bee (Apis mellifera) queens to European drones reduces colony defensive behavior. Annals of the Entomological Society of America 86, 352–355.CrossRefGoogle Scholar
Rinderer, T.E., Stelzer, J.A., Oldroyd, B.P., Buco, S.M. & Rubink, W.L. (1991) Hybridization between European and Africanized honey bees in the neotropical Yucatan peninsula. Science 253, 309–311.CrossRefGoogle ScholarPubMed
Rinderer, T.E., Buco, S.M., Rubink, W.L., Daly, H.V., Stelzer, J.A., Riggio, R.M. & Baptista, F.C. (1993) Morphometric identification of Africanized and European honey bees using large reference populations. Apidologie 24, 569–585.CrossRefGoogle Scholar
Rubenstein, H.S. (1982) Bee-sting diseases: who is at risk? what is the treatment? Lancet 1, 496–499.CrossRefGoogle ScholarPubMed
Schiff, N.M. & Sheppard, W.S. (1993) Mitochondrial DNA evidence for the 19th century introduction of African honey bees into the United States. Experientia 49, 530–532.CrossRefGoogle Scholar
Schmidt, J.O. (1992) Allergy to venomous insects. The Hive and the Honey Bee. pp. 1209–1269. Hamilton, IL, Dadant and Sons.Google Scholar
Sheppard, W.S. & McPheron, B.A. (1991) Ribosomal DNA diversity in Apidae. pp. 89–107in Smith, D.R. (ed.) in Diversity in the genus Apis. Oxford, Westview Press & IBH Publishing Co. Pvt. Ltd.Google Scholar
Sheppard, W.S., Rinderer, T.E., Mazzoli, J., Stelzer, J.A. & Shimanuki, H. (1991) Gene flow between African- and European-derived honey bee populations in Argentina. Nature 349, 782–784.CrossRefGoogle Scholar
Smith, D.R., Brown, W.M. & Taylor, O.R. (1989) Neotropical Africanized bees have African mitochondrial DNA. Nature 339, 213–215.CrossRefGoogle ScholarPubMed
Stort, A.C. (1974) Genetic study of aggressiveness of two subspecies of Apis mellifera in Brazil. I. Some tests to measure aggressiveness. Journal of Apicultural Research 13, 33–38.CrossRefGoogle Scholar
Sugden, E.A. & Williams, K.R. (1991) October 15 the day the bee arrived. Gleanings in Bee Culture 119, 18–21.Google Scholar
Walsh, P.S., Metzger, D.A. & Higuchi, R. (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10, 506–513.Google ScholarPubMed