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COI barcode based species-specific primers for identification of five species of stored-product pests from genus Cryptolestes (Coleoptera: Laemophloeidae)

  • Z. Varadínová (a1) (a2), Y. J. Wang (a1), Z. Kučerová (a3), V. Stejskal (a3), G. Opit (a4), Y. Cao (a5), F. J. Li (a5) and Z. H. Li (a1)...

Abstract

Flat grain beetles of the genus Cryptolestes (Coleoptera: Laemophloeidae) are one of the economically most important stored-product pests which feed on many kinds of agricultural products, especially grains. Nine of more than 40 described Cryptolestes species are recognized as stored-product pests and two of the pest species have a cosmopolitan distribution. Given the rapid growth in global trade of food products, ecological barriers to the spread of pests are easily overcome. Therefore, development of reliable systems for routine quarantine inspection and early infestation detection is vital. In the present study, we established a new rapid and accurate cytochrome c oxidase subunit I-based system for molecular identification of five common stored-product Cryptolestes species, namely, Cryptolestes capensis, Cryptolestes ferrugineus, Cryptolestes pusilloides, Cryptolestes pusillus and Cryptolestes turcicus. Five species-specific primer pairs for traditional uniplex polymerase chain reaction assay are described and their specificity and sensitivity for the identification process is evaluated using larval samples of 12 different populations from three continents (Asia, Europe and North America).

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* Author for correspondence Phone: +86-10-62731299 Fax: +86-10-62733404 E-mail: lizh@cau.edu.cn

References

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Armstrong, K.F. & Ball, S.L. (2005) DNA barcodes for biosecurity: invasive species identification. Philosophical Transactions of the Royal Society B-Biological Sciences 360, 18131823.
Banks, H.J. (1979) Identification of stored product Cryptolestes spp. (Coleoptera, Cucujidae) – rapid technique for preparation of suitable mounts. Journal of the Australian Entomological Society 18, 217222.
deWaard, J.R., Mitchell, A., Keena, M.A., Gopurenko, D., Boykin, L.M., Armstrong, K.F., Pogue, M.G., Lima, J., Floyd, R., Hanner, R.H. & Humble, L.M. (2010) Towards a global barcode library for Lymantria (Lepidoptera: Lymantriinae) tussock moths of biosecurity concern. PLoS ONE 5, e14280.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294299.
Halstead, D.G.H. (1993) Keys for the identification of beetles associated with stored products. 2. Laemophloeidae, Passandridae and Silvanidae. Journal of Stored Products Research 29, 99197.
Howe, R.W. & Lefkowitch, L.P. (1957) The distribution of the storage species of Cryptolestes (Col., Cucujidae). Bulletin of Entomological Research 48, 795809.
Jiang, F., Li, Z.H., Deng, Y.L., Wu, J.J., Liu, R.S. & Buahom, N. (2013) Rapid diagnosis of the economically important fruit fly, Bactrocera correcta (Diptera: Tephritidae) based on a species-specific barcoding cytochrome oxidase I marker. Bulletin of Entomological Research 103, 363371.
Jinbo, U., Kato, T. & Ito, M. (2011) Current progress in DNA barcoding and future implications for entomology. Entomological Science 14, 107124.
Kučerová, Z. & Stejskal, V. (2002) Comparative egg morphology of silvanid and laemophloeid beetles (Coleoptera) occurring in stored products. Journal of Stored Products Research 38, 219227.
Lefkovitch, L.P. (1957) The biology of Cryptolestes ugandae Steel & Howe (Coleoptera, Cucujidae), a pest of stored products in Africa. Proceedings of the Zoological Society of London 128, 419430.
Lefkovitch, L.P. (1962) A revision of African Laemophloeinae (Coleoptera: Cucujidae). Bulletin of the British Museum of Natural History (Entomology) 12, 167245.
Lefkovitch, L.P. (1965) Differences between six species of Cryptolestes (Coleoptera, Cucujidae) in susceptibility to methyl bromide vapour. Bulletin of Entomological Research 56, 197200.
Meyer, C.P. & Paulay, G. (2005) DNA barcoding: error rates based on comprehensive sampling. PLoS Biology 3, 22292238.
Morag, N., Saroya, Y., Braverman, Y., Klement, E. & Gottlieb, Y. (2012) Molecular identification, phylogenetic status, and geographic distribution of Culicoides oxystoma (Diptera: Ceratopogonidae) in Israel. PLoS ONE 7(3), e33610.
Nayak, M.K., Holloway, J.C., Emery, R.N., Pavic, H., Bartlet, J. & Collins, P.J. (2013) Strong resistance to phosphine in the rusty grain beetle, Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae): its characterisation, a rapid assay for diagnosis and its distribution in Australia. Pest Management Science 69, 4853.
Nelson, L.A., Wallman, J.F. & Dowton, M. (2007) Using COI barcodes to identify forensically and medically important blowflies. Medical and Veterinary Entomology 21, 4452.
Nowaczyk, K., Obrepalska-Steplowska, A., Gawlak, M., Throne, J.E., Olejarski, P. & Nawrot, J. (2009) Molecular techniques for detection of Tribolium confusum infestations in stored products. Journal of Economic Entomology 102, 16911695.
Obrepalska-Steplowska, A., Nowaczyk, K., Holysz, M., Gawlak, M. & Nawrot, J. (2008) Molecular techniques for the detection of granary weevil (Sitophilus granarius L.) in wheat and flour. Food Additives and Contaminants: Part A 25, 11791188.
Rees, D. (2004) Insects of Stored Products. CSIRO Publishing, Collingwood, Victoria, Australia, 192pp.
Thomas, M.C. (2005) New distribution records for two species of Cryptolestes Ganglbauer (Coleoptera: Laemophloeidae). Insecta Mundi 19(1–2), 88.
Thomas, M.C. & Zimmerman, M.L. (1989) A new species of stored products Cryptolestes from Thailand (Coleoptera: Cucujidae: Laemophloeinae). Journal of Stored Products Research 25, 7779.
Throne, J.E., Doehlert, D.C. & McMullen, M.S. (2002) Susceptibility of commercial oat cultivars to Cryptolestes pusillus and Oryzaephilus surinamensis . Journal of Stored Products Research 39, 213223.
Trematerra, P., Stejskal, V. & Hubert, J. (2011) The monitoring of semolina contamination by insect fragments using the light filth method in an Italian mill. Food Control 22, 10211026.
Virgilio, M., Backeljau, T., Nevado, B. & de Meyer, M. (2010) Comparative performances of DNA barcoding across insect orders. BMC Bioinformatics 11, 206.
Wang, G., Li, C., Guo, X., Xing, D., Dong, Y., Wang, Z., Zhang, Y., Liu, M., Zheng, Z., Zhang, H., Zhu, X., Wu, Z. & Zhao, T. (2012) Identifying the main mosquito species in China based on DNA barcoding. PLoS ONE 7, e47051.
Wang, Y.J., Li, Z.H., Zhang, S.F., Varadínová, Z., Jiang, F., Kučerová, Z., Stejskal, V., Opit, G., Cao, Y. & Li, F.J. (2014) DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae). Bulletin of Entomological Research 104, 671678.
Waugh, J. (2007) DNA barcoding in animal species: progress, potential and pitfalls. BioEssays 29, 188197.
Wells, J. & Williams, D. (2007) Validation of a DNA-based method for identifying Chrysomyinae (Diptera: Calliphoridae) used in a death investigation. International Journal of Legal Medicine 121, 18.
Wiemers, M. & Fiedler, K. (2007) Does the DNA barcoding gap exist? – a case study in blue butterflies (Lepidoptera: Lycaenidae). Frontiers in Zoology 4, 116.
Yang, Q.Q., Kučerová, Z., Li, Z.H., Kalinovic, I., Stejskal, V., Opit, G. & Cao, Y. (2012) Diagnosis of Liposcelis entomophila (Insecta: Psocodea: Liposcelididae) based on morphological characteristics and DNA barcodes. Journal of Stored Products Research 48, 120125.
Yang, Q.Q., Zhao, S., Kučerová, Z., Stejskal, V., Opit, G., Qin, M., Cao, Y., Li, F.J. & Li, Z.H. (2013) Validation of the 16S rDNA and COI DNA barcoding technique for rapid molecular identification of stored product psocids (Insecta: Psocodea: Liposcelididae). Journal of Economic Entomology 106, 419425.

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COI barcode based species-specific primers for identification of five species of stored-product pests from genus Cryptolestes (Coleoptera: Laemophloeidae)

  • Z. Varadínová (a1) (a2), Y. J. Wang (a1), Z. Kučerová (a3), V. Stejskal (a3), G. Opit (a4), Y. Cao (a5), F. J. Li (a5) and Z. H. Li (a1)...

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