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Characterization of microsatellite DNA libraries from three mealybug species and development of microsatellite markers for Pseudococcus viburni (Hemiptera: Pseudococcidae)

Published online by Cambridge University Press:  17 December 2013

M.C.G. Correa ,
T. Zaviezo ,
J. Le Maguet ,
E. Herrbach  and
T. Malausa
M.C.G. Correa*
Affiliation:
Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Casilla 306-22, Santiago, Chile
T. Zaviezo
Affiliation:
Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Casilla 306-22, Santiago, Chile
J. Le Maguet
Affiliation:
Institut National de la Recherche Agronomique, UMR SVQV INRA/UDS, Equipe ViVe. 28, rue de Herrlisheim. BP 20507, 68000 Colmar, France Institut Français des Productions Cidricoles, La Rangée Chesnel, 61500 Sées, France
E. Herrbach
Affiliation:
Institut National de la Recherche Agronomique, UMR SVQV INRA/UDS, Equipe ViVe. 28, rue de Herrlisheim. BP 20507, 68000 Colmar, France
T. Malausa
Affiliation:
Institut National de la Recherche Agronomique, UMR ISA INRA/UNSA/CNRS, Equipe BPI. 400, route des Chappes. BP 167, 06903 Sophia-Antipolis, France
*
*Author for correspondence Phone: +56-2-26864268 Fax: +56-2-25534130 E-mail: macorre1@uc.cl; maggiecorrea@gmail.com
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Abstract

Mealybugs (Hemiptera: Pseudococcidae) are important pests for crops worldwide. Different species, cryptic taxa under the same species name or even populations within a species can differ in biological characteristics, such as phenology, resistance to insecticides, virus transmission and susceptibility to natural enemies. Therefore, their management efficacy depends on their accurate identification. Microsatellite genetic markers are efficient in revealing the fine-scale taxonomic status of insects, both at inter- and intra-specific level. Despite their potential uses, microsatellites have been developed only for one mealybug species so far. Hence, it is unclear whether microsatellites may be useful to assess mealybug population differentiation and structuring. In this work, we tested the feasibility of developing microsatellite markers in mealybugs by: (i) producing and characterizing microsatellite DNA libraries for three species: Pseudococcus viburni, Pseudococcus comstocki and Heliococcus bohemicus, and (ii) by developing and testing markers for Ps. viburni. The obtained libraries contained balanced percentages of dinucleotide (ranging from 15 to 25%) and trinucleotide (from 5 to 17%) motifs. The marker setup for Ps. viburni was successful, although 70% of the primers initially tested were discarded for a lack of polymorphism. Finally, 25 markers were combined in two multiplex polymerase chain reactions with 21 displaying no evidence of deviation from Hardy–Weinberg equilibrium. Ps. viburni markers were tested on one population from France and one from Chile. The markers revealed a significant genetic differentiation between the two populations with an Fst estimate of 0.266.

Keywords

MicrosatelliteSSRnext-generation sequencing454Comstock mealybugObscure mealybugBohemian mealybug
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 104 , Issue 2 , April 2014 , pp. 213 - 220
Copyright
Copyright © Cambridge University Press 2013 

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