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Biotype status and genetic polymorphism of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Greece: mitochondrial DNA and microsatellites

  • A. Tsagkarakou (a1), C.S. Tsigenopoulos (a2), K. Gorman (a3), J. Lagnel (a2) and I.D. Bedford (a4)...

Abstract

The genetic polymorphism and the biotype identity of the tobacco whitefly Bemisia tabaci (Gennadius) have been studied in population samples taken from different localities within Greece from cultivated plants growing in greenhouses or in open environments and from non-cultivated plants. Two different approaches were used: sequencing of the mitochondrial cytochrome oxidase I (mtCOI) gene and genotyping using microsatellite markers. Analyses of the mtCOI sequences revealed a high homogeneity between the Greek samples which clustered together with Q biotype samples that had been collected from other countries. When genetic polymorphism was examined using six microsatellite markers, the Greek samples, which were all characterized as Q biotype were significantly differentiated from each other and clustered into at least two distinct genetic populations. Moreover, based on the fixed differences revealed by the mtCOI comparison of known B. tabaci biotype sequences, two diagnostic tests for discriminating between Q and B and non-Q/non-B biotypes were developed. Implementation of these diagnostic tools allowed an absence of the B biotype and presence of the Q biotype in the Greek samples to be determined.

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*Fax: +302810 245858 E-mail: tsagkarakou@nagref.gr

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Abdullahi, I., Winter, S., Atiri, G.I. & Thottappilly, G. (2003) Molecular characterization of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) populations infesting cassava. Bulletin of Entomological Research 93, 97106.
Abdullahi, I., Atiri, G.I., Thottappilly, G. & Winter, S. (2004) Discrimination of cassava-associated Bemisia tabaci in Africa from polyphagous populations, by PCR–RFLP of the internal transcribed spacer regions of ribosomal DNA. Journal of Applied Entomology 128, 8187.
Avgelis, A.D., Roditakis, N., Dovas, C.I., Katis, N.I., Vassilakos, N. & Bem, F. (2001) First report of Tomato yellow leaf curl virus on tomato crops in Greece. Plant Disease 85, 678.
Bedford, I.D., Briddon, R.W., Markham, P.G., Brown, J.K. & Rosell, R.C. (1993) A new species of Bemisia or biotype of Bemisia tabaci (Genn.) as a future pest of European agriculture. Plant Health and the European Single Market, BCPC Monograph 54, 381386.
Bedford, I.D., Briddon, R.W., Brown, J.K., Rosell, R.C. & Markham, P.G. (1994) Geminivirus transmission and biological characterization of Bemisia tabaci (Gennadius) biotypes from different geographic regions. Annals of Applied Biology 125, 311325.
Brown, J.K. (2000) Molecular markers for the identification and global tracking of whitefly vector-Begomovirus complexes. Virus Research 71, 233260.
Brown, J.K., Coats, S.A., Bedford, I.D., Markham, P.G., Bird, J. & Frohlich, D.R. (1995) Characterization and distribution of esterase electromorphs in the whitefly, Bemisia tabaci (Genn.) (Homoptera: Aleyrodidae). Biochemical Genetics 33, 205214.
Brown, J.K., Perring, T.M., Cooper, A.D., Bedford, I.D. & Markham, P.G. (2000) Genetic analysis of Bemisia tabaci (Hemiptera: Aleyrodidae) populations by isoelectric focusing electrophoresis. Biochemical Genetics 38, 1325.
Byrne, F.J. & Devonshire, A.L. (1993) Insensitive acetylcholinesterase and esterase polymorphism in susceptible and resistant populations of the tobacco whitefly Bemisia tabaci (Genn). Pesticide Biochemistry and Physiology 45, 3442.
Cervera, M.T., Cabezas, J.A., Simón, B., Martínez-Zapater, J.M., Beitia, F. & Cenis, J.L. (2000) Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis. Bulletin of Entomological Research 90, 391396.
De Barro, P.J. (2005) Genetic structure of the whitefly Bemisia tabaci in the Asia-Pacific region revealed using microsatellite markers. Molecular Ecology 14, 36953718.
De Barro, P.J., Driver, F., Trueman, J.W.H. & Curran, J. (2000) Phylogenetic relationships of world populations of Bemisia tabaci (Gennadius) using ribosomal ITS1. Molecular Biology and Evolution 16, 2936.
De Barro, P.J., Trueman, J.W.H. & Frohlich, D.R. (2005) Bemisia argentifolii is a race of B. tabaci (Hemiptera: Aleyrodidae): the molecular genetic differentiation of B. tabaci populations around the world. Bulletin of Entomological Research 95, 193203.
Delatte, H., Reynaud, B., Granier, M., Thornary, L., Lett, J.M., Goldbach, R. & Peterschmitt, M. (2005) A new silverleaf-inducing biotype Ms of Bemisia tabaci (Hemiptera: Aleyrodidae) indigenous to the islands of the south-west Indian Ocean. Bulletin of Entomological Research 95, 2935.
Evanno, G., Regnaut, S. & Goudet, J. (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology 14, 26112620.
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783791.
Fisher, R.A. (1970) Statistical methods for research workers. 14th edn. Edinburgh, Oliver & Boyd.
Frohlich, D.R., Torres-Jerez, I., Bedford, I.D., Markham, P.G. & Brown, J.K. (1999) A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology 8, 16831691.
Guirao, P., Beitia, F. & Cenis, J.L. (1997) Biotype determination of Spanish populations of Bemisia tabaci (Hemiptera: Aleyrodidae). Bulletin of Entomological Research 87, 587593.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 9598.
Higgins, D.G., Thompson, J.D. & Gibson, T.J. (1996) Using CLUSTAL for multiple sequence alignments. Methods in Enzymology 266, 383402.
Horowitz, A.R., Denholm, I., Gorman, K., Cenis, J.L., Kontsedalov, S. & Ishaaya, I. (2003) Biotype Q of Bemisia tabaci identified in Israel. Phytoparasitica 31, 9498.
Horowitz, A.R., Kontsedalov, S., Khasdan, V. & Ishaaya, I. (2005) Biotypes B and Q of Bemisia tabaci and their relevance to neonicotinoid and pyriproxyfen resistance. Archives of Insect Biochemistry and Physiology 58, 216225.
Huelsenbeck, J.P. & Crandall, K.A. (1997) Phylogeny estimation and hypothesis testing using maximum likelihood. Annual Review of Ecology and Systematics, 28, 437466.
Jones, D.R. (2003) Plant viruses transmitted by whiteflies. European Journal of Plant Pathology 109, 195219.
Khasdan, V., Levin, I., Rosner, A., Morin, S., Kontsedalov, S., Maslenin, L. & Horowitz, A.R. (2005) DNA markers for identifying biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) and studying population dynamics. Bulletin of Entomological Research 95, 605613.
Kirk, A.A., Lacey, L.A., Roditakis, N. & Brown, J.K. (1993) The status of Bemisia tabaci (Hom.: Aleyrodidae), Trialeurodes vaporariorum (Hom.: Aleyrodidae) and their natural enemies in Crete. Entomophaga 38, 405410.
Legg, J.P., French, R., Rogan, D., Okao-Okuja, G. & Brown, J.K. (2002) A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda. Molecular Ecology 11, 12191229.
Markham, P.G., Bedford, I.D., Liu, S. & Pinner, M.S. (1994) The transmission of geminiviruses by Bemisia tabaci. Pesticide Science 42, 123128.
Martin, J.H., Mifsud, D. & Rapisarda, C. (2000) The whiteflies (Hemiptera: Aleyrodidae) of Europe and the Mediterranean Basin. Bulletin of Entomological Research 90, 407448.
Moriones, E. & Navas-Castillo, J. (2000) Tomato yellow leaf curl virus, an emerging virus complex causing epidemics worldwide. Virus Research 71, 123134.
Moya, A., Guirao, P., Cifuentes, D., Beitia, F. & Cenis, J.L. (2001) Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera: Aleyrodidae) based on random amplified polymorphic DNA–polymerase chain reaction. Molecular Ecology 10, 891897.
Perring, T.M. (2001) The Bemisia tabaci species complex. Crop Protection 20, 725737.
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817818.
Pritchard, J.K., Stephens, M. & Donnelly, P. (2000) Inference of population structure using multilocus genotype data. Genetics 155, 945959.
Raymond, M. & Rousset, F. (1995a) Genepop version 2.0: population genetics software for exact tests and ecumenicism. Journal of Heredity 86, 248249.
Raymond, M. & Rousset, F. (1995b) An exact test for population differentiation. Evolution 49, 12801283.
Roberts, R.J., Vincze, T., Posfai, J. & Macelis, D. (2005) REBASE-restriction enzymes and DNA methyltransferases. Nucleic Acids Research 33, D230D232.
Roditakis, E., Roditakis, N. & Tsagkarakou, A. (2005) Insecticide resistance in Bemisia tabaci (Homoptera: Aleyrodidae) populations from Crete. Pest Management Science 61, 577582.
Rodríguez, F., Oliver, J.F., Marín, A. & Medina, J.R. (1990) The general stochastic model of nucleotide substitution. Journal of Theoretical Biology 142, 485501.
Rousset, F. & Raymond, M. (1995) Testing heterozygote excess and deficiency. Genetics 140, 14131419.
Rosenberg, N.A. (2002) Distruct: a program for the graphical display of structure results http://www.cmb.usc.edu/~noahr/distruct.html.
Simón, B., Cenis, J.L., Demichelis, S., Rapisarda, C., Caciagli, P. & Bosco, D. (2003) Survey of Bemisia tabaci (Hemiptera: Aleyrodidae) biotype in Italy with the description of a new biotype (T) from Euphorbia characias. Bulletin of Entomological Research 93, 259264.
Swofford, D.L. (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods) 4.0b10a. Sunderland, Massachusetts, Sinauer Associates.
Swofford, D.L., Olsen, G.J., Waddel, P.J. & Hillis, D.M. (1996) Phylogenetic inference. pp. 407514in Hillis, D.M., Moritz, C. & Mable, B.K. (Eds) Molecular systematics. Sunderland, Massachusetts, Sinauer.
Tsagkarakou, A. & Roditakis, N. (2003) Isolation & characterization of microsatellite loci in Bemisia tabaci (Hemiptera: Aleyrodidae). Molecular Ecology Notes 3, 196198.
Weir, B.S. & Cockerham, C.C. (1984) Estimating F-statistics for the analysis of population structure. Evolution 38, 13581370.
Žanić, K., Cenis, J.L., Kačić, S. & Katalinić, M. (2005) Current status of Bemisia tabaci in coastal Croatia. Phytoparasitica 33, 6064.

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