Skip to main content Accessibility help
×
×
Home

Analysis of the complete genome sequence of black queen cell virus JL1 from infected honeybees in China

  • Q. Yang (a1), Z.-Y. Song (a1), X. Feng (a2), J. Zhang (a3), Y. Zheng (a1), X.-H. Wang (a1), J.-C. Sui (a1), Z.-G. Wang (a1) and Y. Sun (a1)...

Abstract

There are six strains of the complete genomic sequences of black queen cell virus (BQCV) published in the GenBank, including South Africa (AF183905), South Korea (JX149531), Hungary 10 (EF517515), Poland 4 (EF517519), Poland 5 (EF517520) and Poland 6 (EF517521). Based on the six BQCV strains published in the GenBank, ten pairs of primers were designed in the present study using reverse transcription polymerase chain reaction to obtain the first complete genome sequence of a BQCV strain in China, called the BQCV China-JL1 strain (KP119603). A phylogenetic tree was then built to analyse their genetic relationships. The BQCV China-JL1 strain showed 86–93% similarity with the six strains published in the GenBank. The BQCV China-JL1 strain consisted of 8358 nucleotides (nt). The 5′-proximal open reading frame (ORF1) initiated at nt position 546 and terminated at nt position 4676, ORF3 initiated at nt position 4891 and terminated at nt position 5433, and the 3′-proximal ORF (ORF2) was located between nt positions 5750 and 8203.

Copyright

Corresponding author

*Author for correspondence Phone: 86-431-87607322 Fax: 86-431-87607989 E-mail: feng_xin@jlu.edu.cn

References

Hide All
Antúnez, K., D'Alessandro, B., Corbella, E., Ramallo, G. & Zunino, P. (2006) Honeybee viruses in Uruguay. Journal of Invertebrate Pathology 93, 6770.
Bailey, L. (1982) Viruses of honeybees. Bee World 63, 165173.
Bailey, L. & Woods, R.D. (1977) Two more small RNA viruses from honey bees and further observations on sacbrood and acute bee-paralysis viruses. The Journal of General Virology 37, 175182.
Baker, A.C. & Schroeder, D.C. (2008) Occurrence and genetic analysis of picorna-like viruses infecting worker bees of Apis mellifera L. populations in Devon, South West England. Journal of Invertebrate Pathology 98, 239242.
Benjeddou, M., Leat, N., Allsopp, M. & Davison, S. (2001) Detection of acute bee paralysis virus and black queen cell virus from honeybees by reverse transcriptase PCR. Applied and Environmental Microbiology 67(5), 23842387.
Berényi, O., Bakonyi, T., Derakhshifar, I., Köglberger, H. & Nowotny, N. (2006) Occurrence of six honeybee viruses in diseased Austrian apiaries. Applied and Environmental Microbiology 72, 24142420.
Chantawannakul, P., Ward, L., Boonham, N. & Brown, M. (2006) A scientific note on the detection of honeybee viruses using real-time PCR (TaqMan) in Varroa mites collected from a Thai honeybee (Apis mellifera) apiary. Journal of Invertebrate Pathology 91, 6973.
Chen, Y.P. & Siede, R. (2007) Honey bee viruses. Advances in Virus Research 70, 3380.
Forgach, P., Bakonyi, T., Tapaszti, Z., Nowotny, N. & Rusvai, M. (2008) Prevalence of pathogenic bee viruses in Hungarian apiaries: situation before joining the European Union. Journal of Invertebrate Pathology 98, 235238.
Haddad, N., Brake, M. & Migdadi, H. (2008) First detection of honey bee viruses in Jordan by RT-PCR. Jordan Journal of Agricultural Sciences 4(3), 242246.
Higes, M., Esperón, F. & Sánchez-Vizcaíno, J.M. (2007) First report of black queen-cell virus detection in honey bees (Apis mellifera) in Spain. Spanish Journal of Agricultural Research 5(3), 322325.
Hong, I.P., Woo, S.O., Choi, Y.S., Han, S.M., Kim, N.S., Kim, H.K., Han, S.H., Lee, M.Y., Lee, M.L. & Byeon, K.H. (2011) Prevalence of Nosema and virus in honey bee (Apis mellifera L.) colonies on flowering period of Acacia in Korea. Mycobiology 39(4), 317320.
Kajobe, R., Marris, G., Budge, G., Laurenson, L., Cordoni, G., Jones, B., Wilkins, S., Cuthbertson, A.G.S. & Brown, M.A. (2010) First molecular detection of a viral pathogen in Ugandan honey bees. Journal of Invertebrate Pathology 104(2), 153156.
Kojima, Y., Toki, T., Morimoto, T., Yoshiyama, M., Kimura, K. & Kadowaki, T. (2011) Infestation of Japanese native honey bees by Tracheal mite and virus from nonnative European honey bees in Japan. Microbial Ecology 62, 895906.
Kukielka, D., Perez, A.M., Higes, M., Bulboa, M.C. & Sánchez-Vizcaíno, J.M. (2008) Analytical sensitivity and specificity of a RT-PCR for the diagnosis and characterization of the spatial distribution of three Apis mellifera viral diseases in Spain. Apidologie 39, 607617.
Leat, N., Ball, B., Govan, V. & Davison, S. (2000) Analysis of the complete genome sequence of black queen-cell virus, a picorna-like virus of honey bees. The Journal of General Virology 81, 21112119.
Mayo, M.A. (2002) Virus taxonomy-Houston. Archives of Virology 147(5), 10711076.
Nielsen, S.L., Nicolaisen, M. & Kryger, P. (2008) Incidence of acute bee paralysis virus, black queen cell virus, chronic bee paralysis virus, deformed wing virus, Kashmir bee virus and sacbrood virus in honeybees (Apis mellifera) in Denmark. Apidologie 39, 310.
Reddy, K.E., Noh, J.H., Choe, S.E., Kweon, C.H., Yoo, M.S., Doan, H.T., Ramya, M., Yoon, B.S., Nguyen, L.T., Nguyen, T.T., Quyen, D.V., Jung, S.C., Chang, K.Y. & Kang, S.W. (2013) Analysis of the complete genome sequence and capsid region of black queen cell viruses from infected honeybees (Apis mellifera) in Korea. Virus Genes 47(1), 126132.
Runckel, C., Flenniken, M.L., Engel, J.C., Ruby, J.G., Ganem, D., Andino, R. & DeRisi, J.L. (2011) Temporal analysis of the honey bee microbiome reveals four novel viruses and seasonal prevalence of known viruses, Nosema and Crithidia. PLoS ONE 6, e20656.
Sanpa, S. & Chantawannakul, P. (2009) Survey of six bee viruses using RT-PCR in Northern Thailand. Journal of Invertebrate Pathology 100, 116119.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28(10), 27312739.
Tapaszti, Z., Forgach, P., Kovago, C., Topolska, G., Nowotny, N., Rusvai, M. & Bakonyi, T. (2009) Genetic analysis and phylogenetic comparison of Black queen cell virus genotypes. Veterinary Microbiology 139, 227234.
Teixeira, E.W., Chen, Y., Message, D., Pettis, J. & Evans, J.D. (2008) Virus infections in Brazilian honey bees. Journal of Invertebrate Pathology 99, 117119.
Tentcheva, D., Gauthier, L., Zappulla, N., Dainat, B., Cousserans, F., Colin, M.E. & Bergoin, M. (2004) Prevalence and seasonal variations of six bee viruses in Apis mellifera and Varroa destructor mite populations in France. Applied and Environmental Microbiology 70, 71857191.
Welch, A., Drummond, F., Tewari, S., Averill, A. & Burand, J.P. (2009). Presence and prevalence of viruses in local and migratory honeybees (Apis mellifera) in Massachusetts. Applied and Environmental Microbiology 75(24), 78627865.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Bulletin of Entomological Research
  • ISSN: 0007-4853
  • EISSN: 1475-2670
  • URL: /core/journals/bulletin-of-entomological-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed