Skip to main content Accessibility help
×
Home
Hostname: page-component-77ffc5d9c7-5dsxc Total loading time: 0.544 Render date: 2021-04-22T13:31:45.821Z Has data issue: false Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }

Genetic diversity and insecticide resistance of Myzus persicae (Hemiptera: Aphididae) populations from tobacco in Chile: evidence for the existence of a single predominant clone

Published online by Cambridge University Press:  09 March 2007

E. Fuentes-Contreras
Affiliation:
Departamento de Producción Agrícola, Facultad de Ciencias Agrarias, Universidad de Talca, Casilla 747, Talca, Chile
C.C. Figueroa
Affiliation:
Instituto de Ecología y Evolución, Universidad Austral de Chile, Casilla 567, Valdivia, Chile
M. Reyes
Affiliation:
Departamento de Producción Agrícola, Facultad de Ciencias Agrarias, Universidad de Talca, Casilla 747, Talca, Chile
L.M. Briones
Affiliation:
Instituto de Ecología y Evolución, Universidad Austral de Chile, Casilla 567, Valdivia, Chile
H.M. Niemeyer
Affiliation:
Laboratorio de Química Ecológica, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
Corresponding
E-mail address:

Abstract

The tobacco-feeding race of Myzus persicae (Sulzer), formerly known as M. nicotianae Blackman, was introduced into Chile during the last decade. In order to evaluate the genetic diversity and insecticide resistance status of Chilean tobacco aphid populations, a field survey was conducted in 35 tobacco fields covering a 300 km latitudinal survey. The populations sampled were characterized using microsatellite markers and morphometric multivariate analysis. Insecticide resistance levels were assessed through a microplate esterase assay and the mutation status of the kdr gene. All samples collected corresponded to the same anholocyclic aphid genotype, and showed morphological variation within the range expected for the tobacco-feeding race of M. persicae. Esterase activity showed the level and variability expected for an R1 clone lacking mutations in the sodium channels (susceptible kdr), thus corresponding to a type slightly resistant to organophosphate and carbamate, and susceptible to pyrethroid insecticides.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2004

Access options

Get access to the full version of this content by using one of the access options below.

References

Al-Aboodi, A. & ffrench-Constant, R.H. (1995) RAPD PCR confirms absence of genetic variation between insecticide resistant variants of the green peach aphid, Myzus persicae (Homoptera: Aphididae). Great Lakes Entomologist 28, 127133.Google Scholar
Blackman, R.L. (1987a) Morphological discrimination of a tobacco-feeding form from Myzus persicae (Sulzer) (Hemiptera: Aphididae), and a key to New World Myzus (Nectarosiphon) species. Bulletin of Entomological Research 77, 713730.CrossRefGoogle Scholar
Blackman, R.L. (1987b) Rearing and handling aphids. pp. 5968in Minks, A.K. & Harrewijn, P. (Eds) Aphids, their biology, natural enemies and control. Vol. B. Amsterdam, Elsevier Science Publishers B.V.Google Scholar
Blackman, R.L. & Eastop, V.F. (2000) Aphids on the world's crops. An identification guide. 2nd edn. Chichester, John Wiley & Sons Ltd.Google Scholar
Brookes, C.P. & Loxdale, H.D. (1987) Survey of enzyme variation in British populations of Myzus persicae (Sulzer) (Hemiptera: Aphididae) on crops and weed hosts. Bulletin of Entomological Research 77, 8389.CrossRefGoogle Scholar
Casals, P. & Silva, G. (1999) Susceptibilidad a insecticidas de Myzus persicae Sulzer en remolacha. Agrociencia (Chile) 15, 5561.Google Scholar
ClementsK.M., K.M.,SorensonC.E., C.E.,WiegmannB.M., B.M.,Neese, P.A. & Roe, R.M. (2000a) Genetic, biochemical, and behavioral uniformity among populations of Myzus nicotianae and Myzus persicae. Entomologia Experimentalis et Applicata 95, 269281.CrossRefGoogle Scholar
ClementsK.M., K.M.,WiegmannB.M., B.M.,SorensonC.E., C.E.,SmithC.F., C.F.,Neese, P.A. & Roe, R.M. (2000b) Genetic variation in the Myzus persicae complex (Homoptera: Aphididae): evidence for a single species. Annals of the Entomological Society of America 93, 3146.CrossRefGoogle Scholar
DevonshireA.L., A.L.,Devine, G.J. & Moores, G.D. (1992) Comparison of microplate esterase assays and immunoassay for identifying insecticide resistant variants of Myzus persicae (Homoptera: Aphididae). Bulletin of Entomological Research 82, 459463.CrossRefGoogle Scholar
DevonshireA.L., A.L.,FieldL.M., L.M.,FosterS.P., S.P.,MooresG.D., G.D.,Williamson, M.S. & Blackman, R.L. (1998) The evolution of insecticide resistance in the peach–potato aphid, Myzus persicae. Philosophical Transactions of the Royal Society of London, Series Biological Sciences 353, 16771684.CrossRefGoogle Scholar
FentonB., B.,Woodford, J.A.T. & Malloch, G. (1998) Analysis of clonal diversity of the peach–potato aphid, Myzus persicae (Sulzer), in Scotland, UK and evidence for the existence of a predominant clone. Molecular Ecology 7, 14751487.CrossRefGoogle Scholar
FentonB., B.,MallochG., G.,NavajasM., M.,Hillier, J. & Birch, A.N.E. (2003) Clonal composition of the peach–potato aphid Myzus persicae (Homoptera: Aphididae) in France and Scotland: comparative analysis with IGS fingerprinting and microsatellite markers. Annals of Applied Biology 142, 255267.CrossRefGoogle Scholar
Field, L.M. & Foster, S.P. (2002) Amplified esterase genes and their relationship with other insecticide resistance mechanisms in English field populations of the aphid, Myzus persicae (Sulzer). Pest Management Science 58, 889894.CrossRefGoogle Scholar
FigueroaC.C., C.C.,Loayza-Muro, R. & Niemeyer, H.M. (2002) Temporal variation of RAPD–PCR phenotype composition on the grain aphid Sitobion avenae (Hemiptera: Aphididae) on wheat: the role of hydroxamic acids. Bulletin of Entomological Research 92, 2534.Google ScholarPubMed
FosterS.P., S.P.,HarringtonR., R.,DevonshireA.L., A.L.,DenholmI., I.,Devine, G.J.Kenward, M.G. & Bale, J.S. (1996) Comparative survival of insecticide-susceptible and resistant peach–potato aphids, Myzus persicae (Sulzer) (Hemiptera: Aphididae), in low temperature field trials. Bulletin of Entomological Research 86, 1727.CrossRefGoogle Scholar
FosterS.P., S.P.,HarringtonR., R.,DevonshireA.L., A.L.,DenholmI., I.,Clark, S.J. & Mugglestone, M.A. (1997) Evidence for a possible fitness trade-off between insecticide resistance and the low temperature movement that is essential for survival of UK populations of Myzus persicae (Hemiptera: Aphididae). Bulletin of Entomological Research 87, 573579.CrossRefGoogle Scholar
FosterS.P., S.P.,DenholmI., I.,HarlingZ.K., Z.K.,Moores, G.D. & Devonshire, A.L. (1998) Intensification of insecticide resistance in UK field populations of the peach–potato aphid, Myzus persicae (Hemiptera: Aphididae) in 1996. Bulletin of Entomological Research 88, 127130.CrossRefGoogle Scholar
FosterS.P., S.P.,WoodcockC.M., C.M.,WilliamsonM.S., M.S.,DevonshireA.L., A.L.,Denholm, I. & Thompson, R. (1999) Reduced alarm response by peach–potato aphids, Myzus persicae (Hemiptera: Aphididae), with knock-down resistance to insecticides (kdr) may impose a fitness cost through increased vulnerability to natural enemies. Bulletin of Entomological Research 89, 133138.CrossRefGoogle Scholar
FosterS.P., S.P.,HarringtonR., R.,DewarA.M., A.M.,Denholm, I. & Devonshire, A.L. (2002) Temporal and spatial dynamics of insecticide resistance in Myzus persicae (Hemiptera: Aphididae). Pest Management Science 58, 895907.CrossRefGoogle Scholar
Fuentes-ContrerasE., E.,BasoaltoE., E.,MuñozC., C.,Gaete-EastmanC., C.,Peralta, G. & Carrasco, C. (2003) Population dynamics and natural enemies of the tobacco aphid (Myzus nicotianae Blackman) in central Chile. In Proceedings of the VI International Aphid Symposium, Rennes, in press.Google Scholar
GuillemaudT., T.,Mieuzet, L. & Simon, J.C. (2003) Spatial and temporal genetic variability in French populations of the peach–potato aphid, Myzus persicae. Heredity 91, 143152.CrossRefGoogle ScholarPubMed
Harlow, C.D. & Lampert, E.P. (1990) Resistance mechanisms in two colour forms of the tobacco aphid (Homoptera: Aphididae). Journal of Economic Entomology 83, 21302135.CrossRefGoogle Scholar
KephalogianniT.E., T.E.,TsitsipisJ.A., J.A.,MargaritopoulosJ.T., J.T.,ZintzarasE., E.,DelonR., R.,Martin, I.B. & Schwaer, W. (2002) Variation in the life cycle and morphology of the tobacco host-race of Myzus persicae (Hemiptera: Aphididae) in relation to its geographical distribution. Bulletin of Entomological Research 92, 301307.CrossRefGoogle ScholarPubMed
Klein, C. & Waterhouse, D.F. (2000) Distribution and importance of arthropods associated with agriculture and forestry in Chile. Canberra, ACIAR Monograph no. 68.Google Scholar
Kolar, C.S. & Lodge, D.M. (2001) Progress in invasion biology: predicting invaders. Trends in Ecology and Evolution 16, 199204.CrossRefGoogle ScholarPubMed
Koziol, F.S. & Semtner, P.J. (1984) Extent of resistance to organophosphorous insecticides in field populations of the green peach aphid (Homoptera: Aphididae) infesting flue-cured tobacco in Virginia. Journal of Economic Entomology 77, 13.CrossRefGoogle Scholar
Lynch, M. (1984) Destabilizing hybridization, general-purpose genotypes and geographic parthenogenesis. Quarterly Review of Biology 59, 257290.CrossRefGoogle Scholar
Margaritopoulos, J.T. & Tsitsipis, J.A. (2002) Production of sexual morphs by apterous virginoparae of Myzus persicae (Hemiptera: Aphididae) in relation to pre- and postnatal exposure to short day conditions. Bulletin of Entomological Research 92, 321330.CrossRefGoogle ScholarPubMed
MargaritopoulosJ.T., J.T.,Zissis, M. & Tsitsipis, J.A. (1998) Attempted discrimination of Myzus persicae and Myzus nicotianae (Homoptera: Aphididae) by random amplified polymorphic DNA chain reaction technique. Annals of the Entomological Society of America 91, 602607.CrossRefGoogle Scholar
MargaritopoulosJ.T., J.T.,TsitsipisJ.A., J.A.,Zintzaras, E. & Blackman, R.L. (2000) Host-correlated morphological variation of Myzus persicae (Hemiptera: Aphididae) populations in Greece. Bulletin of Entomological Research 90, 233244.CrossRefGoogle Scholar
MargaritopoulosJ.T., J.T.,TsitsipisJ.A., J.A.,Goudoudaki, S. & Blackman, R.L. (2002) Life cycle variation of Myzus persicae (Hemiptera: Aphididae) in Greece. Bulletin of Entomological Research 92, 309319.CrossRefGoogle Scholar
MargaritopoulosJ.T., J.T.,BlackmanR.L., R.L.,Tsitsipis, J.A. & Sannino, L. (2003) Co-existence of different host-adapted forms of the Myzus persicae group (Hemiptera: Aphididae) in southern Italy. Bulletin of Entomological Research 93, 131135.CrossRefGoogle Scholar
Martínez-TorresD., D.,CarrioR., R.,LatorreA., A.,SimonJ.C., J.C.,Hermoso, A. & Moya, A. (1997) Assessing the nucleotide diversity of three aphid species by RAPD. Journal of Evolutionary Biology 10, 459477.CrossRefGoogle Scholar
Martínez-TorresD., D.,FosterS.P., S.P.,FieldL.M., L.M.,Devonshire, A.L. & Williamson, M.S. (1999) A sodium channel point mutation is associated with resistance to DDT and pyrethroid insecticides in the peach–potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Insect Molecular Biology 8, 18.CrossRefGoogle Scholar
Mazzoni, E. & Cravedi, P. (2002) Analysis of insecticide-resistant Myzus persicae (Sulzer) populations collected in Italian peach orchards. Pest Management Science 58, 975980.CrossRefGoogle ScholarPubMed
Roush, R.T. & Daly, J.C. (1990) The role of population genetics in resistance research and management. pp. 97152in Roush, R.T. & Tabashnik, B.E. (Eds) Pesticide resistance in arthropods. New York, Chapman and Hall.CrossRefGoogle Scholar
Roush, R.T. & McKenzie, J.A. (1987) Ecological genetics of insecticide and acaricide resistance. Annual Review of Entomology 32, 361380.CrossRefGoogle ScholarPubMed
SambrookJ., J.,Fritsch, E.F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbour, Cold Spring Harbour Laboratory Press.Google Scholar
SemtnerP.J., P.J.,Tilson, W.M. & Dara, S.K. (1998) Performance of the tobacco aphid (Homoptera: Aphididae) on various host-plants. Journal of Entomological Science 33, 180195.CrossRefGoogle Scholar
SloaneM.A., M.A.,SunnucksP., P.,Wilson, A.C.C. & Hales, D.F. (2001) Microsatellite isolation, linkage group identification and determination of recombination frequency in the peach–potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Genetical Research 77, 251260.CrossRefGoogle Scholar
Stevens, M. & Dewar, A. (1996) Pest and disease problems in Chile: what lessons can be learnt? British Sugar Review 64, 3438.Google Scholar
SunnucksP., P.,EnglandP.R., P.R.,Taylor, A.C. & Hales, D.F. (1996) Microsatellite and chromosome evolution of parthenogenetic Sitobion aphids in Australia. Genetics 144, 747756.Google ScholarPubMed
UnruhT., T.,Knight, A. & Bush, M.R. (1996) Green peach aphid (Homoptera: Aphididae) resistance to endosulfan in peach and nectarine orchards in Washington State. Journal of Economic Entomology 89, 10671073.CrossRefGoogle Scholar
Van DoninckK., K.,SchonI., I.,De Bruyn, L. & Martens, K. (2002) A general purpose genotype in an ancient asexual. Oecologia 132, 205212.CrossRefGoogle Scholar
WilsonA.C.C., A.C.C.,SunnucksP., P.,Blackman, R.L. & Hales, D.F. (2002) Microsatellite variation in cyclically parthenogenetic populations of Myzus persicae in southeastern Australia. Heredity 88, 258266.CrossRefGoogle Scholar
WolfM.A., M.A.,Abdel-AalY.A.I., Y.A.I.,GohD.K.S., D.K.S.,Lampert, E.P. & Roe, M. (1994) Organophosphate resistance in the tobacco aphid (Homoptera: Aphididae): purification and characterization of a resistance-associated esterase. Journal of Economic Entomology 87, 11571164.CrossRefGoogle Scholar
ZitoudiK., K.,MargaritopoulosJ.T., J.T.,Mamuris, Z. & Tsitsipis, J.A (2001) Genetic variation in Myzus persicae populations associated with host-plant and life cycle category. Entomologia Experimentalis et Applicata 99, 303311.CrossRefGoogle Scholar
Zúñiga, E. (1969) Huéspedes para Chile del áfido Myzus persicae (Sulzer) (Homoptera: Aphididae). Revista Chilena de Entomología 6, 145146.Google Scholar

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 0
Total number of PDF views: 117 *
View data table for this chart

* Views captured on Cambridge Core between September 2016 - 22nd April 2021. This data will be updated every 24 hours.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Genetic diversity and insecticide resistance of Myzus persicae (Hemiptera: Aphididae) populations from tobacco in Chile: evidence for the existence of a single predominant clone
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Genetic diversity and insecticide resistance of Myzus persicae (Hemiptera: Aphididae) populations from tobacco in Chile: evidence for the existence of a single predominant clone
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Genetic diversity and insecticide resistance of Myzus persicae (Hemiptera: Aphididae) populations from tobacco in Chile: evidence for the existence of a single predominant clone
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *