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Screening of anaerobic gut fungi using a pressure transducer

  • W.-Y. Zhu (a1), M. K. Theodorou (a1), B. B. Nielsen (a1) (a2) and A. P. J. Trinci (a2)

Extract

The growth of gut anaerobic fungi is usually determined in batch culture by gravimetric measurements of dry-matter (DM) loss, or by quantification of fermentation end-products (Lowe et al., 1987). These techniques involve destructive sampling of the cultures and require many replicate cultures in time-course experiments. Moreover, techniques using DM loss are only able to measure the end-point of degradation of substrate and cannot follow the growth over the entire fermentation period. Using a pressure transducer, Theodorou et al. (1994) developed a simple and sensitive gas production procedure to determine the fermentation kinetics of ruminant foods. This procedure can also be used to follow the growth of anaerobic fungi on soluble and particulate substrate in batch cultures Theodorou et al. (1995).

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France, J., Dhanoa, M. S., Theodorou, M. K., Lister, S. J., Davies, R. D. and Isaac, D. 1993. A model to interpret gas accumulation profiles associated with in vitro degradation of ruminant feeds. Journal of Theoretical Biology 163: 99111.
Gordon, G. L. R. and Phillips, M. W. 1989. Degradation and utilization of cellulose and straw by three different anaerobic fungi from the ovine rumen. Applied and Environmental Microbiology 55: 17031710.
Lowe, S. E., Theodorou, M. K. and Trinci, A. P. J. 1987. Growth and fermentation of an anaerobic fungus on various carbon sources and effect of temperature on development. Applied and Environmental Microbiology 53: 12101215.
Nielsen, B. B. 1996. A study of survival, growth and enzyme production in anaerobic fungi. Ph.D. thesis, University of Manchester.
Theodorou, M. K., Davies, R. D., Nielsen, B. B., Lawrence, M. I. G. and Trinci, A. P. J. 1995. Determination of growth of anaerobic fungi on soluble and cellulosic substrates using pressure transducer. Microbiology 141: 671678.
Theodorou, M. K., Williams, B. A., Dhanoa, M. S., McAllan, A. B. and France, J. 1994. A simple gas production method using pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48: 185197.
Webb, J. and Theodorou, M. K. 1991. Neocallimastix hurleyensis sp nov., an anaerobic fungus from the ovine rumen. Canadian Journal of Botany 69: 12201224.
Zhu, W.- Y., Theodorou, M. K., Longland, A. C., Nielsen, B. B., Dijkstra, J. and Trinci, A. P. J. 1996. Growth and survival of anaerobic fungi in batch and continuous-flow cultures. Anaerobe 2: 2937.
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BSAP Occasional Publication
  • ISSN: 0263-967X
  • EISSN: 2053-5953
  • URL: /core/journals/bsap-occasional-publication
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