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Effect of microbial isolates on microbial yield estimation in RUSITEC system

Published online by Cambridge University Press:  27 February 2018

M. D. Carro  and
E. L. Miller
M. D. Carro
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
E. L. Miller
Affiliation:
Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 OJQ
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Extract

The estimation of rumen microbial protein synthesis is one of the main points in the nitrogen (N)-rationing systems for ruminants, as microbial protein provides proportionately 0.4 to 0.9 of amino acids entering the small intestine in ruminants receiving conventional diets (Russell et al., 1992). Methods of estimating microbial protein synthesis rely on marker techniques in which a particular microbial constituent is related to the microbial N content. Marker : N values have generally been established in mixed bacteria isolated from the liquid fraction of rumen digesta and it has been assumed that the same relationship holds in the total population leaving the rumen (Merry and McAllan, 1983). However, several studies have demonstrated differences in composition between solid-associated (SAB) and fluid-associated bacteria in vivo (Legay-Carmier and Bauchart, 1989) and in vitro (Molina Alcaide et al, 1996), as well in marker : N values (Pérez et al., 1996). This problem could be more pronounced in the in vitro semi-continuous culture system RUSITEC, in which there are three well defined components (a free liquid phase, a liquid phase associated with the solid phase and a solid phase), each one having associated microbial populations.

The objective of this experiment was to investigate the effect of using different bacterial isolates (BI) on the estimation of microbial production of four different diets in RUSITEC (Czerkawski and Breckenridge, 1977), using (15NH4)2 SO4 as microbial marker, and to assess what effects any differences would have on the comparison of microbial protein synthesis between diets.

This study was conducted in conjunction with an in vitro experiment described by Carro and Miller (1997). Two 14-day incubation trials were carried out with the rumen simulation technique RUSITEC (Czerkawski and Breckenridge, 1977). The general incubation procedure was the one described by Czerkawski and Breckenridge (1977) and more details about the procedures of this experiment are given elsewhere (Carro and Miller, 1997).

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Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 306 - 308
Copyright
Copyright © British Society of Animal Science 1998

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References

Carro, M. D. and Miller, E. L. 1997. Effect of nitrogen form on growth of rumen micro-organisms in vitro . In In vitro techniques for measuring nutrient supply to ruminants (ed. E. R., Deaville, E., Owen, A. T., Adesogan, C., Rymer, J. A., Huntington and T. L. J., Lawrence), pp. 309–311. British Society of Animal Science occasional publication no. 22.Google Scholar
Czerkawski, J. W. and Breckenridge, G. 1977. Design and development of a long-term rumen simulation technique (Rusitec). British Journal of Nutrition 38:371–384.CrossRefGoogle ScholarPubMed
Komisarczuk, , Sylvie, , Durand, M., Beaumatin, Ph. and Hannequart, G. 1987. Utilisation de lázote 15 pour la mesure de la protéosynthèse microbienne dans les phases solide et liquide dún fermenteur semi-continu (Rusitec). Reproduction, Nutrition and Development 27:261–262.CrossRefGoogle Scholar
Legay-Cannier, F. and Bauchart, D. 1989. Distribution of bacteria in the rumen contents of dairy cows given a diet supplemented with soya-bean oil. British Journal of Nutrition 61: 725–740.CrossRefGoogle Scholar
Merry, R. J. and McAllan, A. B. 1983. A comparison of the chemical composition of mixed bacteria harvested from the liquid and solid fractions of rumen digesta. British Journal of Nutrition 50: 701–709.CrossRefGoogle ScholarPubMed
Minato, H. and Suto, T. 1981. Technique for fractionation of bacteria in rumen microbial ecosystem. IV. Attachment of rumen bacteria to cellulose power and elution of bacteria attached to it. Journal of General Applied Microbiology 27: 21–31.CrossRefGoogle Scholar
Molina Alcaide, E., Weisbjerg, M. R. and Hvelplund, T. 1996. Degradation characteristics of shrubs and the effect of supplementation with urea or protein on micrboial production using a continuous-culture system. Journal of Animal Physiology and Animal Nutrition 75:1221-132.Google Scholar
Perez, J. F., Rodriguez, C. A., Gonzalez, J., Balcells, J. and Guada, J. A. 1996. Contribution of dietary purine bases to duodenal digesta in sheep. In situ studies of purine degradability corrected for microbial contamination. Animal Feed Science and Technology 62:251–262.CrossRefGoogle Scholar
Russel, J. B., O'Connor, J. D., Fox, D. G., Van Soest, P. J. and Sniffen, C. J. 1992. A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. Journal of Animal Science 70:3551–3561.CrossRefGoogle Scholar
Statistical Analysis Systems Institute. 1993. SAS companion for the Microsoft Windows Environment, version 6. SAS Institute Inc. Cary, NC.Google Scholar